The Antimicrobial Susceptibility of Klebsiella pneumoniae from Community Settings in Taiwan, a Trend Analysis

Lin, Wu-Pu; Wang, Jann-Tay; Chang, Shan‐Chwen; Chang, Feng–Yee; Fung, Chang-Phoné; Chuang, Yin-Ching; Chen, Yao-Shen; Shiau, Yih-Ru; Tan, Mei-Chen; Wang, Huiying; Lai, Jui-Fen; Huang, I-Wen; Lauderdale, Tsai‐Ling

doi:10.1038/srep36280

Cited by 47 publications

(42 citation statements)

References 53 publications

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“…Few studies have examined this trend specifically in these resistant bacteria; however, a similar trend for increasing resistance of general K . pneumoniae isolates was found in national surveillance studies from Taiwan 34 and Korea 35 .…”

Section: Discussionmentioning

confidence: 99%

Carbapenem Nonsusceptible Klebsiella pneumoniae in Taiwan: Dissemination and Increasing Resistance of Carbapenemase Producers During 2012–2015

Chiu

Chan

et al. 2018

Sci Rep

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Before 2011, the prevalence rates of carbapenemase-producing Klebsiella pneumoniae (CPKP) among carbapenem nonsusceptible K. pneumoniae (CnSKP) isolates were below 10% in Taiwan. The study presents the dissemination and increased antimicrobial resistance of CPKP from January 2012 to August 2015, as shown by Taiwanese multicenter surveillance. Isolates with minimum inhibitory concentrations (MICs) of >1 μg/mL for imipenem or meropenem were collected, screened for various carbapenemase genes by PCR, and tested for antimicrobial susceptibility. Among 1,457 CnSKP isolates, 1,250 were collected from medical centers. The CnSKP prevalence in medical centers increased by 1.7-fold during the study. Among all CnSKP isolates, 457 were CPKP. The CPKP rate among CnSKP increased by 1.5-fold and reached 36.8% in 2015. The CPKP nonsusceptibility rate to aztreonam, fluoroquinolones, and aminoglycosides increased yearly. Six CPKP isolates carried dual carbapenemase genes. Three Ambler classes were identified in 451 isolates with a single carbapenemase: classes A (315 blaKPC-2, 2 blaKPC-3, 28 blaKPC-17, 2 blaKPC-34), B (26 blaIMP-8, 2 blaNDM-1, 36 blaVIM-1), and D (40 blaOXA-48). The blaOXA-48 rate among CPKP increased by 6-fold over three years. Most KPC and OXA-48 producers were ST11. CnSKP was increasingly prevalent, owing to CPKP dissemination. Additionally, CPKP became more resistant during the study period.

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Section: Discussionmentioning

confidence: 99%

Carbapenem Nonsusceptible Klebsiella pneumoniae in Taiwan: Dissemination and Increasing Resistance of Carbapenemase Producers During 2012–2015

Chiu

Chan

et al. 2018

Sci Rep

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“…K. pneumonia was resistant only to a single antibiotic (gentamicin). Contrary to this study, extended-spectrum β-lactamase producing strains of Klebsiella pneumoniae have been shown to cause resistance to many antibiotics [22,23]. P. aeruginosa showed resistance to 3 antibiotics and intermediate to two antibiotics making it the least susceptible microorganism in the hospital surfaces.…”

Section: Discussionmentioning

confidence: 74%

Isolation, Characterization, and Antimicrobial Profiling of Bacteria Found on Thika Level 5 Hospital Surfaces, Kenya

Nyaruaba¹,

Mwaliko²,

Ngugi³

2019

Preprint

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Multiple studies have shown that hospital settings are poorly cleaned during terminal cleaning. The adequacy of these cleaning methods has been undermined by presence of multi drug resistant bacteria on hospital surfaces. This case is even more serious in developing countries leading to health care- associated infections that pose a great threat to patients, visitors and health care providers in hospital settings.This study used various microbiological techniques to test for antibiotic susceptibility profiles of bacteria present at Thika Level 5 Hospital surfaces, Kenya. A simple random cross sectional study was performed, with a total of 85 samples being collected from five different sites. The sites included male and female wards, health care personnel offices, latrine, and kitchen surfaces. Samples were collected using sterile swabs, dipped in normal saline, and transported to the laboratory within 2Hours for processing.Of the 85 plates cultured, 47 plates showed bacterial growth (55%) on selective media with a significant P value of 0.0357. Seven different species of bacteria were identified biochemically from all sites, Escherichia coli was the most abundant species (28%), and the least was Salmonella typhii (5%). Multiple drug resistance was common in the different bacteria identified. All isolates were resistant to chloramphenical and susceptible to gentamycin. The most resistant microorganism was Staphylococcus aureus (50%), and the least resistant microorganism was Klebsiella pneumoniae (12.5%). The antimicrobial resistant bacterial species identified in this study have been documented to cause serious health care associated infections. These results present a significant public health concern because there is a possibility of patients, staff and visitors contacting nosocomial infections when they come into contact with surfaces at Thika Level 5 Hospital surfaces, Kenya.

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“…cereus. None of the bacterial endophytes had antibacterial activity against K. pneumoniae; this could be due to the resistance mechanism by K. pneumoniae such as development of extended-spectrum β-lactamases (ESBLs) (Carlet, 2012;Lin et al, 2016). The ANOVA statistics analysis indicated that p value was less than 0.05 (p <0.05), therefore there was a significant difference between all five bacterial endophytes antimicrobial activity.…”

Section: Antibacterial Activity Of Endophytes Extracts Against Pathogmentioning

confidence: 99%

Isolation and identification of bacterial endophytes from Crinum macowanii Baker

Morare¹,

Ubomba-Jaswa²,

Serepa-Dlamini³

2018

Afr. J. Biotechnol.

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The widespread distribution of Crinum macowanii across the African continent has entrenched the plant's medicinal usage in treating diverse diseases. While its phytochemistry is well established, its microbial symbionts and their utility have not been described. As such, five bacterial endophytes, viz. Staphylococcus species C2, Staphylococcus species C3, Bacillus species C4, Acinetobacter species C5 and Staphylococcus species C6 were isolated from fresh C. macowanii bulb and their phenotypic and genotypic profiles verified by Gram staining and 16S rRNA gene sequencing; respectively. The latter was used to construct a phylogenetic tree that showed similarities (higher than 50 bootstrap values) among the endophytic bacterial isolates. Chemical analysis of bacterial endophytes was done by extracting the crude extracts of each endophyte. Antibacterial activity of each endophyte was performed against a few selected bacterial pathogenic strains (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus and Bacillus cereus) using the disk diffusion method with Streptomycin used as a positive control. The crude extracts of all the endophytes showed no bioactivity against K. pneumoniae, though the inhibition was observed against E. coli, P. aeruginosa, S. aureus and B. cereus. These results suggest that crude extracts of endophytic bacteria from C. macowanii have the potential to be used as antimicrobial agents.

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The Antimicrobial Susceptibility of Klebsiella pneumoniae from Community Settings in Taiwan, a Trend Analysis

Cited by 47 publications

References 53 publications

Carbapenem Nonsusceptible Klebsiella pneumoniae in Taiwan: Dissemination and Increasing Resistance of Carbapenemase Producers During 2012–2015

Carbapenem Nonsusceptible Klebsiella pneumoniae in Taiwan: Dissemination and Increasing Resistance of Carbapenemase Producers During 2012–2015

Isolation, Characterization, and Antimicrobial Profiling of Bacteria Found on Thika Level 5 Hospital Surfaces, Kenya

Isolation and identification of bacterial endophytes from Crinum macowanii Baker

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