The Antimutagenic Activities of Blackcurrant Extract: Evaluation Using Saccharomyces cerevisiae LOH System

Yamamoto, Ai; Kawaguchi, Satomi; Nakashima, Kana; Kamitai, Yoshihiro; Kato, Yoji

doi:10.3123/jemsge.34.115

Cited by 5 publications

(10 citation statements)

References 43 publications

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Section: Discussionmentioning

confidence: 99%

“…Previously, we reported the antigenotoxic effects of BCE evaluated using yeast LOH assay and human lymphoblastoid cell assays [ 2 , 3 ]. In a yeast gene mutation assay, BCEs from premature and mature fruit, inhibited DNA mutations induced by H 2 O 2 , methyl MMS as an alkylating agent, and UV as physical stress [ 2 ]. Several types of BCEs extracted from mature and premature fruit with or without heat treatment exhibited antigenotoxic and antioxidative activities against H 2 O 2 -induced DNA damage and intracellular ROS in TK6 cells [ 3 ].…”

Section: Discussionmentioning

confidence: 99%

“…Several types of BCEs extracted from mature and premature fruit with or without heat treatment exhibited antigenotoxic and antioxidative activities against H 2 O 2 -induced DNA damage and intracellular ROS in TK6 cells [ 3 ]. BCE contains high concentrations of polyphenolic compound, including anthocyanin, and of L-ascorbic (vitamin C) acid, which exerts antioxidant activity with radical scavenging [ 2 , 3 ]. We evaluated the radical scavenging activity of polyphenolic compounds such as anthocyanins and catechin.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, we reported that extracts of mature and premature blackcurrant produced in Aomori Prefecture had high anti-oxidant and anti-genotoxic activities, as evaluated using a yeast loss-of-heterozygosity (LOH) assay, an in vitro comet assay to evaluate DNA damage, and a micronucleus (MN) test to screen for genotoxic compounds in human TK6 lymphoblastoid cells [ 2 , 3 ]. In the yeast LOH assay, blackcurrant extracts (BCEs) extracted from premature and mature fruits suppressed gene mutations induced by hydrogen peroxide (H 2 O 2 ), methyl methanesulfonate (MMS), and ultraviolet (UV) radiation [ 2 ]. The BCEs showed antigenotoxic effects, with or without heat treatment, against H 2 O 2 -induced oxidative stress in human lymphoblastoid cells, as determined using comet and MN assays [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

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Radioprotective activity of blackcurrant extract evaluated by in vitro micronucleus and gene mutation assays in TK6 human lymphoblastoid cells

et al. 2017

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IntroductionBlackcurrant (Ribs nigrum L.) is a classical fruit that has long been used to prepare juice, jam, liqueur, and sometimes medicines in Europe. Previously, we reported a genome defense effect by the antioxidative activity of several types of blackcurrant extracts (BCEs) in yeast and human cell gene mutation assays. In this study, we determined if BCE exerted radioprotective activity against DNA damage, chromosomal aberration, and gene mutations in the TK6 human lymphoblastoid cell line. We prepared aqueous BCE extracted from mature fruits cultivated in the Aomori Prefecture, Japan.FindingsIn the micronucleus test and TK gene mutation assay, TK6 cells were irradiated with 0, 0.125, 0.250, 0.500, and 1.000 Gy with or without 1.0 mg/mL BCE. Intracellular hydrogen peroxide (H2O2) was measured using the fluorescent probe BES-H2O2-Ac. Induction of micronuclei and gene mutations by γ-irradiation exposure was suppressed in combination with BCE. In addition, BCE reduced intracellular H2O2 levels caused by γ-irradiation.ConclusionsOur findings clearly support the genome defense potential of blackcurrant against γ-induced DNA damage. We postulate that these genome defense activities are related to the antioxidant compounds in blackcurrant.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Radioprotective activity of blackcurrant extract evaluated by in vitro micronucleus and gene mutation assays in TK6 human lymphoblastoid cells

et al. 2017

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“…The inhibition ratio was calculated using the formula below. AS is the absorbance of the sample and AC is the absorbance of the control, in which there are no antioxidant reagents [ 11 , 12 ]. …”

Section: Methodsmentioning

confidence: 99%

Genotoxicity-suppressing effect of aqueous extract of Connarus ruber cortex on cigarette smoke-induced micronuclei in mouse peripheral erythrocytes

et al. 2015

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IntroductionAccording to published information, it has not been determined whether the inhalation of cigarette smoke can induce chromosome aberrations and/or point mutations in mice, though cigarette smoke is clearly carcinogenic to mice. We tested clastogenicity of inhaled cigarette smoke in mouse by a micronucleus test using peripheral erythrocytes. Since it is important to determine the in vivo anti-genotoxic effect against inhaled cigarette smoke to reduce the risk of tobacco carcinogenesis, we also tested in vivo anti-gnotoxic effect against inhaled cigarette smoke of a Connarus extract whose in vitro anti-genotoxic effect was shown.ResultsMale ICR mice were exposed for 1 min to a 6-fold dilution of the smoke once a day for up to 14 consecutive days. Although the frequencies of reticulocytes with micronucleus (MNRETs) and erythrocytes with micronuclei (MN erythrocytes) did not increase within 72 h after a single inhalation of cigarette smoke, the frequency of MN erythrocytes increased significantly upon inhalation for 7 and 14 days. When the Connarus extract was fed to mice at >23.7 ppm during the inhalation period of 14 days, frequency of MN erythrocytes was significantly lower than that at 0 ppm. In vitro antioxidant activity of Connarus extract was almost same to that of vitamin C. The antioxidant activity of the Connarus extract might play an important role in its anti-genotoxic effect against cigarette smoke in vivo, like vitamins C.ConclusionsConsecutive inhalation of cigarette smoke is clastogenic to mouse bone marrow as shown by the increased frequency of MN erythrocytes. Also, it was shown the possibility that the Connarus extract reduces the risk of tobacco carcinogenesis.

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Acidic growth conditions stabilize the ribosomal RNA gene cluster and extend lifespan through noncoding transcription repression

Hasegawa,

Ooka,

Wakatsuki

et al. 2023

Genes to Cells

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Blackcurrant (Ribes nigrum L.) is a classical fruit that has long been used to make juice, jam, and liqueur. Blackcurrant extract is known to relieve cells from DNA damage caused by hydrogen peroxide (H2O2), methyl methane sulfonate (MMS), and ultraviolet (UV) radiation. We found that blackcurrant extract (BCE) stabilizes the ribosomal RNA gene cluster (rDNA), one of the most unstable regions in the genome, through repression of noncoding transcription in the intergenic spacer (IGS) which extended the lifespan in budding yeast. Reduced formation of extrachromosomal circles (ERCs) after exposure to fractionated BCE suggested that acidity of the growth medium impacted rDNA stability. Indeed, alteration of the acidity of the growth medium to pH ~4.5 by adding HCl increased rDNA stability and extended the lifespan. We identified RPD3 as the gene responsible for this change, which was mediated by the RPD3L histone deacetylase complex. In mammals, as inflammation sites in a tissue are acidic, DNA maintenance may be similarly regulated to prevent genome instability from causing cancer.

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The Antimutagenic Activities of Blackcurrant Extract: Evaluation Using Saccharomyces cerevisiae LOH System

Cited by 5 publications

References 43 publications

Radioprotective activity of blackcurrant extract evaluated by in vitro micronucleus and gene mutation assays in TK6 human lymphoblastoid cells

Radioprotective activity of blackcurrant extract evaluated by in vitro micronucleus and gene mutation assays in TK6 human lymphoblastoid cells

Genotoxicity-suppressing effect of aqueous extract of Connarus ruber cortex on cigarette smoke-induced micronuclei in mouse peripheral erythrocytes

Acidic growth conditions stabilize the ribosomal RNA gene cluster and extend lifespan through noncoding transcription repression

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