The Antinociceptive Effect of Light-Emitting Diode Irradiation on Incised Wounds Is Correlated with Changes in Cyclooxygenase 2 Activity, Prostaglandin E2, and Proinflammatory Cytokines

Chia, Yuan-Yi; Liu, Chien Cheng; Feng, Guan; Tseng, Chia Chih; Hung, King-Chü; Chen, Chih-Chieh; Tan, Ping-Heng

doi:10.1155/2017/4792489

Cited by 9 publications

(5 citation statements)

References 39 publications

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“…Colon tissue samples were lysed in a RIPA buffer containing a 2% phosphatase inhibitor (Thermo Scientific) and a 1% mammalian‐protease inhibitor (Sigma‐Aldrich). The membranes were incubated with the following primary antibodies overnight 28 : Invitrogen (1:100): Zonula Occludens‐1 (ZO‐1) and Occludin (1:100, Invitrogen); Claudin‐1 and Junctional adhesion molecule‐A (JAMA, 1:500, Invitrogen); ChAT, NGF, GDNF, sub‐P, tryptase, and GAPDH (1:100, 1:10 000‐internal loading control, Abcam); and PGE2 polyclonal antibody (1:200) (Bioss Inc) 29 . They were incubated with ECL AP‐conjugated anti‐rabbit/mouse IgG (1:3000; GE Healthcare) for 90 minutes in room temperature.…”

Section: Methodsmentioning

confidence: 99%

Sacral nerve stimulation ameliorates colonic barrier functions in a rodent model of colitis

Gharibani

Yin

et al. 2020

Neurogastroenterology Motil

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Background The mucosal barrier damage is recognized as one of the key factors in the pathogenesis of colitis. While sacral nerve stimulation (SNS) was reported to have therapeutic potential for colitis, its mechanisms of actions on colonic permeability remained largely unknown. Methods In this study, colitis was induced by intrarectal administration of TNBS in rats. Five days later, they were treated with SNS or sham‐SNS for 10 days. The effects of SNS on colonic permeability were assessed by measuring the expression of tight‐junction proteins involved in regulating permeability and the FITC‐dextran test. The mechanism of actions of SNS was investigated by studying the function of the enteric nervous system (ENS) cells and analyzing the autonomic nervous system. Key Results SNS decreased the disease activity index, microscopic and macroscopic scores, myeloperoxidase activity, and pro‐inflammatory cytokines (TNF‐α, IL‐6). SNS increased the expression of Zonula Occludens‐1, Occludin, Claudin‐1, and Junctional adhesion molecule‐A in the colon tissue. The FITC‐dextran test showed that the colonic permeability was lower with SCS than sham‐SNS. SNS increased ChAT, pancreatic polypeptide, and GDNF and reduced norepinephrine NGF, sub‐P, and mast cell overactivation in the colon tissue. Concurrently, SNS increased acetylcholine in colon tissues and elevated vagal efferent activity. Conclusions & Inferences SNS ameliorates colonic inflammation and enhances colonic barrier function with the proposed mechanisms involving the increase in parasympathetic activity and modulation of the activity of the ENS and immune system, including mast cells.

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Section: Methodsmentioning

confidence: 99%

Sacral nerve stimulation ameliorates colonic barrier functions in a rodent model of colitis

Gharibani

Yin

et al. 2020

Neurogastroenterology Motil

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“…#3025S, Cell signaling); PGES2 (Cat. #bs-2639R, Bioss) [86, 87]; and GADPH (Cat.# SC-32233, Santa Cruz Biotechnology). Secondary antibodies used were as follows: goat antirabbit-800 (LI-COR, P/N 925–32211) and donkey antimouse-680 (LI-COR, P/N 925–68075).…”

Section: Methodsmentioning

confidence: 99%

Ablating astrocyte insulin receptors leads to delayed puberty and hypogonadism in mice

et al. 2019

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Insulin resistance and obesity are associated with reduced gonadotropin-releasing hormone (GnRH) release and infertility. Mice that lack insulin receptors (IRs) throughout development in both neuronal and non-neuronal brain cells are known to exhibit subfertility due to hypogonadotropic hypogonadism. However, attempts to recapitulate this phenotype by targeting specific neurons have failed. To determine whether astrocytic insulin sensing plays a role in the regulation of fertility, we generated mice lacking IRs in astrocytes (astrocyte-specific insulin receptor deletion [IRKO GFAP ] mice). IRKO GFAP males and females showed a delay in balanopreputial separation or vaginal opening and first estrous, respectively. In adulthood, IRKO GFAP female mice also exhibited longer, irregular estrus cycles, decreased pregnancy rates, and reduced litter sizes. IRKO GFAP mice show normal sexual behavior but hypothalamic-pituitary-gonadotropin (HPG) axis dysregulation, likely explaining their low fecundity. Histological examination of testes and ovaries showed impaired spermatogenesis and ovarian follicle maturation. Finally, reduced prostaglandin E synthase 2 (PGES2) levels were found in astrocytes isolated from these mice, suggesting a mechanism for low GnRH/luteinizing hormone (LH) secretion. These findings demonstrate that insulin sensing by astrocytes is indispensable for the function of the reproductive axis. Additional work is needed to elucidate the role of astrocytes in the maturation of hypothalamic reproductive circuits.

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“…In addition to opioid receptors, current research has also found that low-level laser therapy's inhibitory effect on pain may be related to cytochrome c oxidase (44), cyclooxygenase (35), and various proinflammatory cytokines (45). The underlying molecular mechanism behind the analgesic effect of CO 2 laser irradiation remains unclear.…”

Section: Discussionmentioning

confidence: 99%

Unraveling the parameters and biological mechanisms of CO2 laser therapy for acute pain relief

Wang,

Liu,

Wang

et al. 2023

Front. Neurol.

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Acute pain-related pathology is a significant challenge in clinical practice, and the limitations of traditional pain-relief drugs have made it necessary to explore alternative approaches. Photobiomodulation (PBM) therapy using CO2 laser has emerged as a promising option. In this study, we aimed to identify the optimal parameters of CO2 laser irradiation for acute pain relief through in vivo and in vitro experiments. First, we validated the laser intensity used in this study through bone marrow mesenchymal stem cells (BMSCs) experiments to ensure it will not adversely affect stem cell viability and morphology. Then we conducted a detailed evaluation of the duty cycle and frequency of CO2 laser by the hot plate and formalin test. Results showed a duty cycle of 3% and a frequency of 25 kHz produced the best outcomes. Additionally, we investigated the potential mechanisms underlying the effects of CO2 laser by immunohistochemical staining, and found evidence to suggest that the opioid receptor may be involved in its analgesic effect. In conclusion, this study provides insights into the optimal parameters and underlying mechanisms of CO2 laser therapy for effective pain relief, thereby paving the way for future clinical applications.

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The Antinociceptive Effect of Light-Emitting Diode Irradiation on Incised Wounds Is Correlated with Changes in Cyclooxygenase 2 Activity, Prostaglandin E2, and Proinflammatory Cytokines

Cited by 9 publications

References 39 publications

Sacral nerve stimulation ameliorates colonic barrier functions in a rodent model of colitis

Sacral nerve stimulation ameliorates colonic barrier functions in a rodent model of colitis

Ablating astrocyte insulin receptors leads to delayed puberty and hypogonadism in mice

Unraveling the parameters and biological mechanisms of CO2 laser therapy for acute pain relief

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