The Antinociceptive Effect of the Leaves and Flowers Ethanolic Extracts of Tabebuia aurea (Silva Manso) Benth & Hook. F. ex S. Moore

Abstract

In view of the traditional use of Tabebuia aurea for treating pain and inflammation, the antinociceptive pharmacological potential of T. aurea ethanolic extracts (TAEE) was investigated through in vivo experimental models. First, the MTT assay was conducted to determine the potential cytotoxicity of the TAEEs. Afterwards, the acetic acid-induced writhing test and the formalin-, and glutamate-induced nociception tests were performed on Swiss adult mice treated with TAEEs (100 and 200 mg/kg doses, p.o.), or saline solution (control groups, 10mL/kg, p.o.), or standard drugs: dipyrone 40 mg/kg (p.o.), and morphine 5,7 mg/kg (i.p). In the MTT assay, none of the tested concentrations demonstrated signals of cytotoxicity. In the in vivo experimental models of acetic acid-induced writhing and glutamate-induced nociception, all TAEEs doses were able to statistically reduce the nociceptive response. However, the TAEEs did not show significant decrease in the amount of time that the animals spent licking the stimulated paw in the neurogenic phase of formalin-induced nociception test, differently of what was observed in the inflammatory phase. The results showed that T. aurea species induce an antinociceptive effect in rodents, which encourages the study of new drugs and contributes to the research on natural products.

Amazonian useful plants described in the book “Le Pays des Amazones” (1885) of the Brazilian propagandist Baron de Santa-Anna Nery: a historical and ethnobotanical perspective

“…Sandwith (= Tecoma insignis Miq.) ipê-tabaco 106 Stem bark Native Purgative; dust causes sneezing Antihyperuricemic, anti-inflammatory [ 48 , 49 ]; antinociceptive [ 50 ] Tecoma sp. ipê branco 106 Stem bark Naturalized Purgative Antidiarrheal [ 51 ] Caricaceae Carica papaya L. mamoeiro 102 Unripe fruit Naturalized Vermifuge Anthelmintic † [ 52 , 53 ]; hypoglycemic [ 54 ]; immunomodulatory [ 55 ]; antithrombocytopenic [ 56 ] Chrysobalanaceae Couepia sp.…”