The Antiobesity Effects of Buginawa in 3T3-L1 Preadipocytes and in a Mouse Model of High-Fat Diet-Induced Obesity

Abstract

There has been a remarkable interest in finding lipid inhibitors from natural products to replace synthetic compounds, and a variety of oriental medicinal herbs are reported to have biological activity with regard to lipid inhibition. Buginawa (Bugi) is a novel combined formula that contains twelve medicinal herbs with potential for weight loss induction. We hypothesized that Bugi may have antiobesity effects in 3T3-L1 preadipocytes and in a high-fat diet- (HFD-) induced mouse model. In this study, 3T3-L1 cells were treated with varied concentrations of Bugi (62.5, 125, or 250 μg/mL). Bugi treatment inhibited adipocyte differentiation by suppressing adipogenic transcription genes, including peroxisome proliferator-activated receptor γ protein (PPARγ), CCAAT/enhancer-binding protein α (C/EBPα), sterol regulatory element-binding protein 1 (SREBP1), and CCAAT/enhancer-binding protein β (C/EBPβ). Mice were fed a normal diet or an HFD for 11 weeks, and Bugi was simultaneously administered at 50 or 100 mg/kg. Bugi administration significantly reduced body weight gain and white adipose tissue (WAT) weight and effectively inhibited lipid droplet accumulation in epididymal white adipose tissue (eWAT) and liver tissue. Further, Bugi treatment suppressed mRNA levels of PPARγ, C/EBPα, and SREBP1 in eWAT and liver tissue. Our findings demonstrate that Bugi could be an effective candidate for preventing obesity and related metabolic disorders.

In vivo and in vitro effects of chronical exposure to nonylphenol on lipid metabolism

“…4 Serum CEBPα, FAS, PPARγ, and SREBP1 levels in rats exposed to NP (n = 10). a vs C group, P < 0.05, b vs L group, P < 0.05, c vs M group, P < 0.05 In the current research on the association between NP and obesity, exposure to NP during perinatal period increased body weight, fat weight, serum total cholesterol and glucose levels [24]; NP could be a potential chemical stressor for obesity and obesity-related diseases. However, in some experiments, the exposure duration of NP was short, mostly acute and subacute, and the NP dose was high.…”

“…The exposure dose increased, the red color became deeper, and the fat cells were more densely distributed; WB results showed the protein expressions of CEBPα, FAS, SREBP1, and PPARγ increased in adipocytes. 3T3-L1 pre-adipocyte in vitro experiments are often used to evaluate the interference of environmental chemicals on adipogenesis, and provide a basis for environmental substances that can affect lipid metabolism in the body [5,10,24]. Studies have shown that [11,17]: 4-NP increased PPARγ activity at 1 μM, and significantly promoted triglyceride accumulation in 3T3-L1 adipocytes at 10 μM.…”

Pharmacological Properties of a Traditional Korean Formula Bojungchiseup-tang on 3T3-L1 Preadipocytes and High-Fat Diet-Induced Obesity Mouse Model

“…3T3-L1 preadipocytes were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA; CL-173) and were cultured in DMEM containing 10% BS, 1% ABAM, 1 g/l HEPES, and 1.5 g/l sodium bicarbonate. The 3T3-L1 adipocyte differentiation was performed as previously described [22]. To stimulate adipocyte differentiation, cells were seeded at a density of 2 × 10 5 per well into 6-well plates to confluence (day 0).…”

“…Cell viability was performed was previously described [22]. 3 T3-L1 preadipocytes (1 × 10 4 cells per well) were seeded on 96-well plates at density 1 × 10 4 cells per well.…”

“…Oil Red O Staining of 3T3-L1 Adipocytes. The Oil Red O staining in 3 T3-L1 adipocytes was performed as previously described [22]. 3 T3-L1 preadipocytes were cultured with or without differentiation conditions in the presence or absence of indicated concentrations of BJT.…”