The Antioxidant, Analgesic, Anti-Inflammatory, and Wound Healing Activities of Haplophyllum tuberculatum (Forsskal) A. Juss Aqueous and Ethanolic Extract

Abstract

Herbal extracts are part of the solution to the increased demand for organic health care products. Traditionally, the different extracts prepared from Haplophyllum tuberculatum (Forsskal) A. Juss (H. tuberculatum) have been widely used to treat a wide range of illnesses. The aim of this study is to evaluate the antioxidant, analgesic, anti-inflammatory, and wound healing potential of the aqueous (HTAE) and ethanolic (HTEE) extracts of this plant as well as identify its major phytochemical components using LC-MS. Phytochemical analysis of both extracts revealed a rich composition and especially high amounts of glycosylic flavonols, 65.37% and 68.77% for the HTEE and HTAE, respectively. The antioxidant assays performed (DPPH, FRAP and TAC) indicated the excellent activity of the ethanolic extract while the in vivo activities (analgesic, anti-inflammatory, and healing potential) indicated the excellent activity of the aqueous extract. These findings support the therapeutic use of this plant by preventing pain and inflammation and promoting wound healing. To uncover, identify, and isolate compounds of potential medicinal and therapeutic significance, more studies on this species are required.

Phenolic Profiling, Antioxidant, and Antibacterial Activities of Selected Medicinal Plants from Tunisia

“…Briefly, our study confirmed previous findings reported by Boughalleb et al, [18] , Al-Saeghi et al, [28] Agour et al, [29] Sharifi-Rad et al, [30] Benchikha et al, [31] and Makhlouf-Gafsi et al [32] Generally, to assess the antioxidant potential of bioactive extracts, several factors are involved such as solvent polarity, extraction methods, plant matrix and especially phenolic compounds containing including caffeic acid, 4-feruloylquinic acid, flavonoids, alkaloids and carotenoids. [16,[33][34][35]…”

“…According to their various therapeutic activities including antioxidant, anti‐inflammatory, hypoglycemic, Alzheimer's disease and other brain dysfunctions, as well as prevention of cancer and cardiovascular diseases [50] . Generally, the highest number of phenolic acids was identified in H. tuberculatum (DE: 72.73 % and UAE: 70.00 %) and P. equisetiforme (DE:63.64 % and UAE: 70.00 %), the same outcomes were achieved by Mahmoudi et al., [19] and Agour et al [29] . Unlike, only 27.27 % of total standard compounds were identified in M. arvensis (DE).…”

Biological Evaluation and Structural Analysis of Some Aminodiphenylamine Derivatives

“…We also obtained a stock solution of 2,2-diphenyl-1-picrylhydrazyl free radical (DPPH) in the same solvent, at a concentration of 2 × 10 −4 M. To 1.8 mL of DPPH solution was added 0.2 mL of each solution of the compounds 1 – 9 and we left the mixture in the dark for 30 min, followed by measurements of the absorbance at λ max 517 nm. We obtained the TAC values using Equation (3), which is a common way to assess the antioxidant activity [ 18 , 19 ].…”

Wound Healing Properties of Natural Products: Mechanisms of Action

“…Extracts rich in phenolic compounds from Diospyros mespiliformis , Haplophyllum tuberculatum, and Mangifera indica showed important antioxidant, anti-inflammatory, and antimicrobial activities when evaluated in different models of wound healing [ 106 , 107 , 108 ]. Rocha et al [ 109 ] evaluated the antioxidant, anti-inflammatory, and healing activities of Artemisia campestris L. hydrodistillation residual water and its essential oil, finding that the residual hydrodistillation water showed promising antioxidant and anti-inflammatory activities without causing toxicity in the macrophages and fibroblasts.…”