The antioxidant and antialzhemier activities of the Diplotaenia turcica with phytochemical analysis

Abstract

Diplotaenia turcica is an endemic plant that grows in eastern Turkey. This herb is used in herbal cheese, in meals and in traditional therapies. In this study, we aimed to examine some of the biochemical activities of this plant. Liquid chromatography-mass spectrometry (LC-MS) analysis was conducted on hydro alcohol extract of aerial part of DT. This analysis was applied to determine the total phenolic and flavonoid content, antioxidant and anti-alzheimer activities. LC-MS analysis showed that malic acid and quinic acid were found to be major compounds. The key flavonoids detected were hesperidine and rutine. The end of examination, the total amount of phenolic compound of extract was measured as 27.54 μg PEs/mg. And the total flavonoid amount was measured as 7.31 μg KEs/mg. β-carotene-linoleic acid test, DPPH free radical scavenging method, and ABTS cation radical scavenging outcomes were determined as IC50 of 169.71 μg/mL, 164.42 and 68.74 μg/mL, respectively. Cholinesterase inhibition test which indicate of alzheimer's did not inhibit acetylcholinesterase (AChE), but butyrylcholinesterase (BCHE) enzyme inhibition was 76.57%. As a result, further studies are needed in order to use Diplotaenia turcica plant for treatment or support purposes in the health field.