The Antioxidant and Antigenotoxic Potential of Peltigera Canina and Umbilicaria Nylanderiana Based on Their Phenolic Profile

Emsen, Bugrahan

doi:10.31925/farmacia.2019.5.24

Cited by 12 publications

(5 citation statements)

References 22 publications

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“…Total oxidant and antioxidant levels (TOS (14) and TAS (15) (Rel Assay Diagnostics)), glutathione peroxidase (GPx (Randox Ransel)) (16) and superoxide dismutase (SOD (Randox Ransod)) (17) tissue protein hemolysate, haemoglobin levels were determined with the spectrophotometric method using an autoanalyzer; malondialdehyde (MDA) (18) and catalase (CAT) (19) were assessed with the manual spectrophotometric method.…”

Section: Evaluation Of Biochemical Parametersmentioning

confidence: 99%

The effects of melatonin and thymoquinone on doxorubicin-induced cardiotoxicity in rats

PEHLİVAN>¹,

Durdağı²,

Oyar³

et al. 2020

BLL

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OBJECTIVES: This study aims to investigate the protective effects of thymoquinone and melatonin on the heart against doxorubicin-induced cardiotoxicity in rats. BACKGROUND: Melatonin and thymoquinone may play an important role in cardiotoxicity. METHODS: The subjects were divided into four groups: Control (physiological serum on 5th day), Doxorubicin (DXR), Doxorubicin+Melatonin (DXR+MEL, 10 mg/kg melatonin, intraperitoneally), and Doxorubicin+Thymoquinone (DXR+TQ, 50 mg/kg thymoquinone, orally). On the 5th day of the experiment, all groups were injected with 45 mg/kg DXR into the tail vein. On the 8th day of the experiment, ECG recordings were performed under anaesthesia. RESULTS: Thymoquinone reduced the PR, QRS and QTc intervals, which were increased by DXR, while melatonin only reduced the QTc interval. Melatonin had a protective effect against the histopathological changes induced by DXR, while TQ did not demonstrate such an effect. DXR increased CK-MB, IL-6, MDA, IL-1, IL-18 levels and decreased SOD in the cardiac tissue. MEL reduced the levels of CK-MB, MDA, NO, SOD, IL-1, IL-6, IL-18. Meanwhile, TQ only reduced CK-MB, IL-1 and IL-18. CONCLUSION: Our study showed that DXR induces cardiac injury and that melatonin improves biochemical parameters and offers histological protection; while thymoquinone improves ECG parameters and causes partial recovery of biochemical parameters (Tab. 4, Fig. 2, Ref. 41).

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Section: Evaluation Of Biochemical Parametersmentioning

confidence: 99%

The effects of melatonin and thymoquinone on doxorubicin-induced cardiotoxicity in rats

PEHLİVAN>¹,

Durdağı²,

Oyar³

et al. 2020

BLL

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“…The absorbance of the supernatant was read at λ = 540 nm. The percentage of haemolysis was determined using the following formula: Haemolysis% = (Abscontrol/Abssample) × 100, Reducing power assay The presence of antioxidants in the extracts resulted in reduction of the ferric cyanide complex (Fe 3+ ) to the ferrous cyanide form (Fe 2+ ) [15]. A volume of 0.5 mL of the different concentrations (0.01 -0.1 mg/mL) of the extract was added to 1.25 mL of a phosphate buffer (0.2 M, pH 6.6) mixed with 1.25 mL of potassium ferricyanide (1%).…”

Section: Haemolysis Assaymentioning

confidence: 99%

Phytochemical Profile, Antioxidant, Analgesic and Hypolipidaemic Effects of Ephedra Alata Decne. Female Cones Extract

Chouikh¹

2020

FARMACIA

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The aim of this study is the estimation of the antioxidant, analgesic and hypolipidemic effects and the chromatographic analysis of the extract of female cones of Ephedra alata which is growing in South East Algeria. The extract of E. alata showed better inhibition of DPPH• radical, the highest reductive activity and gave the best protection of erythrocytes. The HPLC analyses has identified eight phenolic compounds: gallic acid, chlorogenic acid, vanillic acid, vanillin, p-coumaric acid, rutin, naringenin and quercetin. The results of the protective effect of hyperlipidaemia caused by Triton X-100 showed that there are noticeable changes in the biochemical and lipid parameters, also for the parameters of oxidative stress in the group exposed to Triton X-100. Also, the treatment with the methanol extract of E. alata yielded similar results to the control batches by normalizing and decreasing the biochemical, stress and lipid parameters. The histological study confirmed the biochemical results. According to the results of the analgesic activity, the extract of E. alata induced a significant decrease in abdominal writhings compared to the control group and the values obtained are very close to those obtained with indomethacin. Rezumat Scopul acestui studiu a fost evaluarea efectelor antioxidante, analgezice, hipolipemiante și analiza cromatografică a extractului de conuri femele de Ephedra alata, din sud-estul Algeriei. Extractul de E. alata a arătat o inhibiție mai bună a radicalului DPPH •, cea mai ridicată activitate reducătoare și a oferit cea mai bună protecție asupra eritrocitelor. Analizele HPLC au identificat opt compuși fenolici: acid galic, acid clorogenic, acid vanilic, vanilină, acid p-cumaric, rutină, naringenină și quercetină. Rezultatele efectului protector asupra hiperlipemiei cauzate de Triton X-100 au arătat că există modificări vizibile ale parametrilor biochimici și lipidici, de asemenea, pentru parametrii stresului oxidativ din grupul expus la Triton X-100. De asemenea, tratamentul cu extract metanolic de E. alata a scăzut parametrii biochimici, stresul oxidativ și parametrii lipidici. Studiul histologic a confirmat rezultatele biochimice. Acțiunea analgezică a extractului de E. alata a indus o scădere semnificativă a contorsiunilor comparativ cu grupul martor și valorile obținute sunt foarte apropiate de cele obținute în cazul administrării de indometacin.

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“…DPPH Radical Scavenging Activity. DPPH • (1,1diphenyl-2-picrilhydrazil) radical scavenging activity is one of the most frequently used methods in studies carried out for determining the antioxidant activity [5]. It is based on colour phase alternation in purple formed by DPPH • with the interaction of antioxidants and radicals after which absorbance value decreases.…”

Section: Lc-ms/ms Instrument and Chromatographic Conditionsmentioning

confidence: 99%

Biological Activities and Phytochemical Screening of Ethanol Extracts From Adonis Paryadrica (Ranunculaceae)

Üçüncü¹

2020

FARMACIA

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In this study, secondary metabolite profiles of flower, leaf and root of Adonis paryadrica ethanolic extracts were performed by using a newly developed and validated LC-MS/MS method. For this purpose, the LC-MS/MS system was used for the quantitative and qualitative analysis of 37 phytochemicals. The main component of the flower, leaf and root extracts was the quinic acid (160, 111 and 64 mg/kg, respectively). Antioxidant activities were determined using methods such as DPPH (SC50, mg/mL), FRAP (µM TEAC), CUPRAC (µM TEAC), ABTS (SC50, mg/mL) and TPC (GAE, µg/mL). While the highest antioxidant activity value for FRAP, CUPRAC, ABTS and TPC methods was determined in the leaf part of the plant, this value was determined in the flower part for DPPH. The antibacterial effects of the plant extracts were determined by liquid microdilution and agar diffusion methods. As a result, antimicrobial effect of flowers, leaf and all sections of plant extracts were determined except plant root extracts which did not display any activity against bacteria. Antimicrobial activity of the plant flower extract was found to be higher especially against to P. vulgaris with 3.5 mg/mL MIC. Rezumat În acest studiu, au fost evaluați metaboliții secundari din extractele etanolice provenite de la florile, frunzele și rădăcinile de Adonis paryadrica, utilizând o metodă LC-MS/MS. În acest scop, s-au pus în evidență 37 fitocompuși, componenta principală fiind acidul quinic (160, 111 și respectiv 64 mg/kg). Activitatea antioxidantă a extractelor a fost determinată prin metode precum DPPH, FRAP, CUPRAC, ABTS și TPC. Extractul de frunze a prezentat activitatea antioxidantă cea mai pronunțată prin metodele FRAP, CUPRAC, ABTS și TPC, iar extractul alcoolic de flori prin metoda DPPH. Efectele antibacteriene ale extractelor au fost determinate prin metoda microdiluției și cea difuzimetrică. Cu excepția extractului de rădăcină, toate celelalte au prezentat activitate antibacteriană. Cea mai mare valoare a CMI pentru P. vulgaris a fost obținută pentru extractul de flori.

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The Antioxidant and Antigenotoxic Potential of Peltigera Canina and Umbilicaria Nylanderiana Based on Their Phenolic Profile

Cited by 12 publications

References 22 publications

The effects of melatonin and thymoquinone on doxorubicin-induced cardiotoxicity in rats

The effects of melatonin and thymoquinone on doxorubicin-induced cardiotoxicity in rats

Phytochemical Profile, Antioxidant, Analgesic and Hypolipidaemic Effects of Ephedra Alata Decne. Female Cones Extract

Biological Activities and Phytochemical Screening of Ethanol Extracts From Adonis Paryadrica (Ranunculaceae)

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