The Antioxidant, Anti-Diabetic, and Anti-Adipogenesis Potential and Probiotic Properties of Lactic Acid Bacteria Isolated from Human and Fermented Foods

Abstract

In this study, lactic acid bacteria (LAB) strains derived from human and fermented food sources were examined to identify their properties related to obesity, as well as establish their safety and stability as probiotics. LAB (Lacticaseibacillus rhamnosus MG4502, Lactobacillus gasseri MG4524, Limosilactobacillus reuteri MG5149, and Weissella cibaria MG5285) exhibited antioxidant activity through DPPH (>26.1%) and ABTS (>40.1%) radical scavenging assays and α-glucosidase inhibitory activities (>60.3%), respectively. The LAB strains promoted anti-adipogenesis by reducing lipid accumulation in 3T3-L1 cells by Oil Red O staining (>70.3%). In addition, we found that these LAB strains were resistant to simulated gastric and intestinal fluids (pH 3, 4, 7, and 8) and showed potential for health promotion, based on hemolysis, cell adhesion, antibiotic susceptibility, and enzyme production. Thus, LAB may be used as probiotic ingredients with beneficial effects.

Probiotics Alleviate Oxidative Stress in H2O2-Exposed Hepatocytes and t-BHP-Induced C57BL/6 Mice

“…Probiotics, especially lactic acid bacteria, are live microorganisms that balance the microflora in the gut when administered in appropriate amounts [7]. Among them, probiotics belonging to the genera Lactobacillus and Bifidobacterium have various biological effects [8]. It is well known that the basic health-promoting function of probiotics is to reduce intestinal-related diseases by regulating and improving the intestinal microbial balance and strengthening the intestinal wall in humans [9].…”

Antifungal properties of whey fermented by lactic acid bacteria in films for the preservation of cheese slices

“…This activity is given by various molecules such as lactate oxidase, catalases, superoxide dismutase, exopolysaccharides, ferulic acid and carotenoids (Jeong et al . 2021). The study of the DPPH radical‐scavenging activity performed on MRS broth by a different L. plantarum strain evidenced relative antioxidant activities of 76% (Won et al .…”

“…Other authors report that the direct antioxidant activity of of LAB ranged values from 20 to 30% (Jeong et al . (2021). Several studies also report the capacity of the LAB to increase the total phenolic compound concentration, such as Mantzourani et al .…”

Lactic Acid Bacteria Exert a Hepatoprotective Effect against Ethanol-Induced Liver Injury in HepG2 Cells

“…The survival rate in simulated GIT was evaluated according to the Maragkoudakis's method with slight modifications [20]. Briefly, the LAB were cultured for 18 h and washed twice with PBS (pH 7.4) after centrifugation (4000× g for 5 min at 4 • C).…”