The antioxidant effects of pumpkin seed oil on subacute aflatoxin poisoning in mice

Abstract

This study was aimed at the investigation of the antioxidant effect of pumpkin seed oil against the oxidative stress-inducing potential of aflatoxin. For this purpose, 48 male BALB/c mice were used. Four groups, each comprising 12 mice, were established. Group 1 was maintained as the control group. Group 2 was administered with pumpkin seed oil alone at a dose of 1.5 mL/kg.bw/day (∼1375mg/kg.bw/day). Group 3 received aflatoxin (82.45% AFB1 , 10.65% AFB2 , 4.13% AFG1, and 2.77% AFG2 ) alone at a dose of 625 μg/kg.bw/day. Finally, group 4 was given both 1.5 mL/kg.bw/day pumpkin seed oil and 625 μg/kg.bw/day aflatoxin. All administrations were oral, performed with the aid of a gastric tube and continued for a period of 21 days. At the end of day 21, the liver, lungs, kidneys, brain, heart, and spleen of the animals were excised, and the extirpated tissues were homogenized appropriately. Malondialdehyde (MDA) levels and catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) activities were determined in tissue homogenates. In conclusion, it was determined that aflatoxin exhibited adverse effects on most of the oxidative stress markers. The administration of pumpkin seed oil diminished aflatoxin-induced adverse effects. In other words, the values of the group, which was administered with both aflatoxin and pumpkin seed oil, were observed to have drawn closer to the values of the control group.

Alleviation of zearalenone toxicity by modified halloysite nanotubes in the immune response of swine

“…Lipid peroxidation is observed as the peroxidation of unsaturated fatty acids of the cell membrane and organelles by free radicals. Peroxidation is confirmed as damage to protein and lipid distribution, decreased resistance and changes in or disruption of the selective permeability of the cell membrane (Eraslan et al 2013). All these processes alter the extracellular and intracellular ion concentrations and disrupt the function of transporter proteins found in the cell membrane (Evans & Halliwell 2001;Halliwell 2007;Ogino & Wang 2007;Niki 2009).…”

Evaluation of Nephrotoxic Effects of Aflatoxins on Common Carp (Cyprinus carpio)

“…By damaging the digestive system, aflatoxins negatively affect the absorption of nutrients, especially vitamins and minerals. Therefore, a decrease in absorption and level of certain vitamins and minerals might weaken the defense mechanism of the cellular antioxidant system [28]. The reduced capacity of cellular antioxidant defense system and reduced level of vitamins such as vitamin A and E, which have an important role in inhibition, and prevention of lipid peroxidation by aflatoxin may lead to an increase in MDA [28].…”

“…Changes in the activity of antioxidant enzymes and a decrease in non-enzymatic antioxidants caused by aflatoxins [28] might be the main factor in reducing the cellular total antioxidant level. A decrease in the cellular antioxidant capacity is reported in blood cells treated with aflatoxin B1 [26].…”

“…Therefore, a decrease in absorption and level of certain vitamins and minerals might weaken the defense mechanism of the cellular antioxidant system [28]. The reduced capacity of cellular antioxidant defense system and reduced level of vitamins such as vitamin A and E, which have an important role in inhibition, and prevention of lipid peroxidation by aflatoxin may lead to an increase in MDA [28]. The MDA produced during lipid peroxidation may form covalent bonds with other components of the cell membrane and therefore affect the physiological function of cell membranes [27].…”

The evaluation of the healing proprieties of pumpkin and linseed oils on deep second-degree burns in rats

“…Recent studies demonstrating the antioxidant, anti-inflammatory and antibacterial properties for these two extracted oils (Eraslan et al, 2013;Hammar et al, 1999;Kaithwas & Majumdar, 2012;Kaithwas et al, 2011), have given us an impetus to pay a special attention to them. Despite all their characteristics, there have been no systematic studies carried out to evaluate their wound healing potential.…”