The antioxidative and hepatoprotective effects comparison of Chinese angelica polysaccharide(CAP)and selenizing CAP (sCAP) in CCl4 induced hepatic injury mice

Gao, Zhenzhen; Zhang, Chao; Tian, Weijun; Liu, Kuanhui; Hou, Ranran; Yue, Chanjuan; Wu, Yi; Wang, Deyun; Liu, Jiaguo; Hu, Yuanliang; Yang, Ying

doi:10.1016/j.ijbiomac.2017.01.013

Cited by 63 publications

(24 citation statements)

References 40 publications

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“…The MAPK signaling pathway plays an important role in the early stage in ammatory responses and the activation of NF-κB pathway [46]. Previous studies have demonstrated that AP exerted its antiin ammatory effect through repression of NF-κB and MAPK pathways [18,47]. Our data showed that AP restrained the initiation of NF-κB and MAPK pathways on claw dermal cells by decreasing the phosphorylation of IκBα, p65 and ERK, JNK, p38, which is consistent with the previous studies.…”

Section: Discussionsupporting

confidence: 92%

Angelica Polysaccharide Attenuates LPS-Induced Inflammation Response of Primary Dairy Cow Claw Dermal Cells Via NF-κB and MAPK Signaling Pathways

Tian

Liu

et al. 2021

Preprint

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Background: Laminitis, an inflammation of the claw laminae, is one of the major causes of bovine lameness, which can lead to enormous economic losses and animal welfare problems in dairy farms. Angelica polysaccharide (AP) is proved to possess anti-inflammatory properties. But the role of AP on inflammatory response of the claw dermal cells has not been reported. The aim of this study was to investigate the anti-inflammatory effects of AP on lipopolysaccharide (LPS)-induced primary claw dermal cells of dairy cow and clarify the potential mechanisms. In the current research, the primary claw dermal cells were exposed to gradient concentrations of AP (10, 50, 100 µg/mL) in the presence of 10 µg/mL LPS. The levels of cytokines and nitric oxide (NO) were detected with ELISA and Griess colorimetric method. The mRNA expressions of TLR4, MyD88 and chemokines were measured with qPCR. The activation of NF-κB and MAPK signaling pathways was detected with western blotting.Results: The results indicated that AP reduced the production of inflammatory mediators (TNF-α, IL-1β, IL-6 and NO), downregulated the mRNA expression of TLR4, MyD88 and some pro-inflammatory chemokines (CCL2, CCL20, CXCL2, CXCL8, CXCL10), and suppressed the NF-κB and MAPK signaling pathways evidenced by inhibition of the phosphorylation of IκBα, p65 and ERK, JNK, p38.Conclusions: Our results demonstrated that AP may exert its anti-inflammatory effects on claw dermal cells of dairy cow by regulating the NF-κB and MAPK signaling pathways.

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Section: Discussionsupporting

confidence: 92%

Angelica Polysaccharide Attenuates LPS-Induced Inflammation Response of Primary Dairy Cow Claw Dermal Cells Via NF-κB and MAPK Signaling Pathways

Tian

Liu

et al. 2021

Preprint

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“…Angelica sinensis polysaccharide (ASP) is an active constituent extracted from a water extract of Angelica sinensis [9][10][11][12][13], which has been used for the treatments of various diseases, including ethylene glycol-induced calcium oxalate urolithiasis [14], aplastic anemia [15], liver injury [16]. Interestingly, ASP has been validated as an ideal and effective therapy drug for liver injury [16][17][18], specifically, Cao et al report that ASP suppresses oxidative stress and hepatic apoptosis to attenuate acetaminophen-induced liver injury [16], Wang et al find that ASP regulates the IL-22/STAT3 pathway to alleviate CCl(4)-induced liver fibrosis [18], and Gao et al support that ASP ameliorates hepatic injury in mice models [17]. In addition, interplay of autophagy and apoptosis plays important role in regulating the development of liver injury [19][20][21], and to our knowledge, induction of protective autophagy suppressed cell apoptosis [22][23][24], and DB promotes liver injury by inducing apoptotic cell death [4,25,5] and inactivation of autophagy flux [25].…”

Section: Introductionmentioning

confidence: 99%

Angelica sinensis polysaccharide (ASP) attenuates diosbulbin-B (DB)-induced hepatotoxicity through activating the MEK/ERK pathway

Liu

Zheng

et al. 2021

Bioengineered

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Diosbulbin-B (DB) is a promising therapeutic drug for cancer treatment; however, DB-induced hepatotoxicity seriously limits its clinical utilization. Based on this, the present study investigated whether the Angelica sinensis extract, angelica sinensis polysaccharide (ASP), was effective to attenuate DB-induced cytotoxicity in hepatocytes. The primary hepatocytes were isolated from rats and cultured in vitro, which were subsequently treated with high-dose DB (100 μM) and ASP (12 μg/ml) to establish the DB-induced hepatotoxicity models. MTT assay and flow cytometry (FCM) were performed to evaluate cell viability, and the results showed that high-dose DBinduced cell apoptosis and inhibition of proliferation were reversed by co-treating cells with ASP, which were supported by our Western Blot assay data that ASP upregulated Cyclin D1 and CDK2 to abrogate high-dose DB-induced cell cycle arrest. In addition, ASP exerted its regulating effects on cell autophagy, and we found that ASP increased LC3B-II/I ratio and Atg5, but decreased p62 to activate the autophagy flux. Of note, the MEK/ERK pathway could be activated by ASP in the DB-treated hepatocytes, and the protective effects of ASP on high-dose DB-induced hepatocyte death were abolished by co-treating cells with the autophagy inhibitor (3-methyladenine, 3-MA) and MEK/ERK selective inhibitor (SCH772984). Moreover, blockage of the MEK/ERK pathway suppressed cell autophagy in the hepatocytes co-treated with ASP and high-dose DB. Taken together, this in vitro study illustrated that ASP activated the MEK/ERK pathway mediated autophagy to suppress high-dose DB-induced hepatotoxicity.

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“…The Caco-2 cells were cultured in a 25 cm 2 culture flask and exposed to C3G and C3G liposomes for 12 h and collected for the T-AOC and MDA level tests. The T-AOC and MDA levels in Caco-2 cells were measured with T-AOC and MDA analysis kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) following the manufacturer’s protocols 47 , 48 .…”

Section: Methodsmentioning

confidence: 99%

Comparison of antioxidant activity between cyanidin-3-O-glucoside (C3G) liposome and cyanidin-3-O-glucoside (C3G) in 2D and 3D cell cultures

Liang

Guan

Cao

et al. 2018

Preprint

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3The 2D cell culture is the predominant in vitro model for numerous studies. 4 4 However, 2D cell cultures may not accurately reflect the functions of 4 5 three-dimensional (3D) tissues, which have extensive cell-cell and cell-matrix 4 6 interactions; thus, using 2D cell cultures may lead to inaccurate experimental results. 7Therefore, to obtain adequate and detailed information about the antioxidant activity 4 8 of cyanidin-3-O-glucoside (C3G) and C3G liposomes in the 2D and 3D cell culture 4 9 7 1 belongs to the flavonoid family and commonly present in human diet, exhibits 7 2 antiinflammatory and antioxidant effects 6-8 . Some studies have showed that C3G 7 3 protects from the adverse effects of radiation, controls the key aspects of 7 4 tumorigenesis, inhibits proliferation, induces apoptosis of cancer cells, reduces 7 5 oxidative stress, fights H 2 O 2 -induced oxidative stress in human embryonic kidney 7 6 cells, induces cell apoptosis and inhibit cell migration 9-15 . 7 7 Liposomes are vesicles, wherein the small aqueous volumes are surrounded by 7 8 bilayer membranes that are normally composed of phospholipids 16, 17 . Liposomes can 7 9 enhance the stability and bioavailability of encapsulated materials by protecting them 8 0 from external environment factors, making them ideal candidates for drug delivery 8 1 and important in food systemsy 18-21 . 8 2 The two-dimensional (2D) monolayer cell models have low anatomical and 8 3 physiological relevance and remain the predominant in vitro model for numerous 8 4 studies 22, 23 . However, some disadvantages exist in the use of the 2D monolayer cell 8 5 model in vitro in some studies. Growing concerns have been demonstrating that 8 6 monolayer and monotypic (2D) cellular screening assays may not effectively 8 7 reproduce the response of a three-dimensional (3D) solid tumor to pharmacological 8 8 compounds 24, 25 . In cancer research, the limitations of the 2D models are considered 8 9 one major reason for the approximately 95% of potential anticancer drugs failures in 9 0 clinical trials 26 . 9 1 The 3D spheroid cell culture systems are providing new insights into tumor 9 2 biology and also in differentiation, tissue organization, and homeostasis 27, 28 . Cells in 9 3 in vitro 3D culture systems are in league with their counterparts in vivo compared 9 4with cells grown as 2D monolayers, wherein 2D monolayer cells also exhibit altered 9 5 cell cycle durations, morphologies, susceptibility to drugs, metabolism, and gene and 9 6 protein expression levels 29, 30 . Cells cultured as 3D models exhibit features that are 9 7 close to the complex in vivo conditions 31 and have been proven realistic for 9 8 translating the study findings for in vivo applications; moreover, culturing cell lines as 9 93D models induces them to behave a step closer to the natural conditions 32, 33 . The 3D 3 1 9

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The antioxidative and hepatoprotective effects comparison of Chinese angelica polysaccharide(CAP)and selenizing CAP (sCAP) in CCl4 induced hepatic injury mice

Cited by 63 publications

References 40 publications

Angelica Polysaccharide Attenuates LPS-Induced Inflammation Response of Primary Dairy Cow Claw Dermal Cells Via NF-κB and MAPK Signaling Pathways

Angelica Polysaccharide Attenuates LPS-Induced Inflammation Response of Primary Dairy Cow Claw Dermal Cells Via NF-κB and MAPK Signaling Pathways

Angelica sinensis polysaccharide (ASP) attenuates diosbulbin-B (DB)-induced hepatotoxicity through activating the MEK/ERK pathway

Comparison of antioxidant activity between cyanidin-3-O-glucoside (C3G) liposome and cyanidin-3-O-glucoside (C3G) in 2D and 3D cell cultures

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