The Antiplasmodial Activity of Isolates fromAjugaremota

Kuria, K.A.M; Chepkwony, HK; Govaerts, Cindy; Roets, Eugène; Busson, Roger; Witte, Peter de; Zupkó, István; Hoornaert, Georges J.; Quirynen, Ludo; Maes, Louis; Janssens, Leen; Hoogmartens, Jos; Laekeman, Gert

doi:10.1021/np0104626

Cited by 73 publications

(53 citation statements)

References 22 publications

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“…Peoples of local rural areas of Pakistan use extract of this plant for treating numerous neurological diseases (Hassan et al, 1994). Ajuga bracteosa contains diterpenoids and withanolides (Glotter 1991) which are responsible for various biological activities (Gautam et al, 2010;Riaz et al, 2007;Kuria et al, 2002;Ray et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

Physio-chemical evaluation and biological activity of Ajuga bracteosa wall and Viola odoroto Linn

Shad¹,

Zeeshan²,

Fazal³

et al. 2016

Afr. J. Trad. Compl. Alt. Med.

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Background: Ajuga bracteosa and Viola odorata are frequently used by the native people of Swat-Pakistan for the curing of fever, malaria, cough, urinary and stomach disorders with slightly different practice of usage like raw powdered, extracts, decoction etc. Methods and Materials: Disc Diffusion Method was used for determination of antimicrobial activities of both plants. Nutrient Agar Media was used for the culturing and growth of all microbial strains. Vitamin C and minerals contents were determined by standard method of AOAC. Na and K were analyzed by using flame photometric technique. Micro minerals i.e. "Ni, Cr, Fe, Cu, Zn, Mn, Ca, Pb, and Mg" were determined by Atomic Absorption Spectrophotometer (AAS). Total Soluble Solid (TSS) was determined by using abbe refractometer and pH was determined by using pH meter. Results: The present study demonstrates that both plants exhibited antibacterial activities against P. aeruginos, E. coli, S. typhi, B. subtilis and S. aureus. The examined plants showed zone of inhibition for aqueous fraction (50.90, 45.90 %) against P. aeruginosa; for EtOAc fraction (41.37, 57.62%) against C. Albicans and for hexane fraction (25.86, 40.57%) against K. pneumoniae, respectively. Total of 14 different minerals (Na, K, P, Ca, Mg, Fe, Zn, Mn, Co, Cr, Ni, Cu, Pb, Cd) were determined and it was also observed that both the examined plants contained significant level of these analyzed minerals. The subject plants contained highest level of magnesium (295.75, 145.85 mg 100 -1 g) and calcium (212.49, 44.00 mg 100 -1 g) and potassium (152.6, 437.45 mg 100 -1 g) while moderate level P, Zn, Na and lower amount of Cd, Ni, Mn and Cu using Atomic Absorption, Flame Photometry and spectrophotometric techniques. V. odorata was found to contain a higher amount of vitamin C (64.05±12.37mg 100 -1 g) as compared to A. bracteosa (45.45 ± 7.29 mg 100 -1 g). Conclusion: Findings of this study can persuade researchers for future comprehensive phytochemical study of these plants using state of art techniques and instruments, which include not only isolation of secondary metabolites from these plants but biological evaluation of isolated compounds both in vivo and in vitro

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Section: Introductionmentioning

confidence: 99%

Physio-chemical evaluation and biological activity of Ajuga bracteosa wall and Viola odoroto Linn

Shad¹,

Zeeshan²,

Fazal³

et al. 2016

Afr. J. Trad. Compl. Alt. Med.

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“…Most importantly, the leaves are used in the treatment of fevers as a substitute for quinine (http://www.pfaf.org/data base/plants.php?Ajuga+bracteosa). Investigators report that the herb possesses anti-malarial activities (Njoroge and Bussmann 2006;Kuria et al 2001Kuria et al , 2002. The two isolates, ajugarin-1 and ergosterol-5,8-endoperoxide are effective against chloroquine sensitive Plasmodium falciparum (Kuria et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Micropropagation of Ajuga bracteosa, a medicinal herb

Kaul

Das

Srivastava

2013

Physiol Mol Biol Plants

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For conservation and genetic transformation, a successful in vitro micropropagation protocol for Ajuga bracteosa, a medicinal herb has been established for the first time. MS medium supplemented with IAA (2 mg/L) and BA (5 mg/L) induced 100 % shoot regeneration with an average of 41.4 shoots of 8.4 cm per culture. Excised in vitro shoots when transferred to MS + IBA (0.5 mg/L) produced 20 roots/shoot of 20.2 cm average length in 100 % cultures. Of the three explants, leaf, petiole and root, leaf displayed quickest response followed by petiole while root was the slowest. Hardening of plantlets was achieved with 82 % survival. The hardened plants were maintained in pots with garden soil under controlled (Temp. 25±2°C) conditions. RAPD exhibited genetic fidelity with 100 % monomorphism in regenerants.

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“…Ergosterol peroxide inhibited TPA-induced inflammatory ear edema in mice 33 , and it also decreased lipid peroxidation of rat liver microsomes and suppressed proliferation of mouse and human lymphocytes stimulated with mitogens 34,35 . Interestingly, it has been reported to have trypanocidal 36 , antiplasmodial 37 , antimycobacterial 38 , antiviral properties 39 , as well as the inhibitory effects of melanin production and tyrosinase expression 40 . This various biological properties of ergosterol peroxide have made it as a valuable and promising compound in pharmacological research.…”

Section: Discussionmentioning

confidence: 99%

Chemical constituents of Hericium erinaceum associated with the inhibitory activity against cellular senescence in human umbilical vascular endothelial cells

Noh

Yang

Kim

et al. 2015

Journal of Enzyme Inhibition and Medicinal Chemistry

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Hericium erinaceum is an edible and medicinal mushroom widely used in Korea, Japan, and China. On the search for biologically active compounds supporting the medicinal usage, the MeOH extract of the fruiting bodies of H. erinaceum was investigated for its chemical constituents. Six compounds were isolated and identified as hericenone D (1), (22E,24R)-5a,8a-epidioxyergosta-6,22-dien-3b-ol (2), erinacerin B (3), hericenone E (4), hericenone F (5) and isohericerin (6) by comparing their spectroscopic data with previously reported values. The inhibitory effects on adriamycin-induced cellular senescence in human dermal fibroblasts (HDFs) and human umbilical vein endothelial cells (HUVECs) of the isolates (1-6) were studied. Among the isolated compounds, ergosterol peroxide (2) reduced senescence associated b-galactosidase (SA-b-gal) activity increased in HUVECs treated with adriamycin. According to experimental data obtained, the active compound may inspire the development of a new pharmacologically useful substance to be used in the treatment and prevention of age-related diseases. KeywordsErgosterol peroxide, Hericium erinaceum, human dermal fibroblasts, human umbilical vein endothelial cells, senescence associated b-galactosidase activity History

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The Antiplasmodial Activity of Isolates fromAjugaremota

Cited by 73 publications

References 22 publications

Physio-chemical evaluation and biological activity of <i>Ajuga bracteosa</i> wall and <i>Viola odoroto</i> Linn

Physio-chemical evaluation and biological activity of <i>Ajuga bracteosa</i> wall and <i>Viola odoroto</i> Linn

Micropropagation of Ajuga bracteosa, a medicinal herb

Chemical constituents of Hericium erinaceum associated with the inhibitory activity against cellular senescence in human umbilical vascular endothelial cells

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