The antiproliferative activity of aloe-emodin is through p53-dependent and p21-dependent apoptotic pathway in human hepatoma cell lines

Kuo, Po‐Lin; Lin, Ta‐Chen; Lin, Chun‐Ching

doi:10.1016/s0024-3205(02)01900-8

Cited by 172 publications

(142 citation statements)

References 32 publications

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“…In the present study, we found that Aloe emodin significantly inhibits U87 cell proliferation in dose-dependent and time-dependent manners. This result is consistent with previous reports on inhibitory effects of Aloe emodin treatment in various human cancer cells such as hepatoma cells (Kuo et al, 2002), skin cancers (Wasserman et al, 2002), gastric cancers (Qin et al, 2010), leukaemic cells (Tabolacci et al, 2011) and breast cancer cells (Huang et al, 2013). Our study also demonstrated that the IC 50 at 24 hours of Aloe emodin treatment in U87 cells was much higher as compared to other human cancer cells such as lung squamous cancers (Lee, 2001), liver cancers (Kuo et al, 2002) and tongue squamous cancers (Chiu et al, 2009).…”

Section: Discussionsupporting

confidence: 94%

Expression Profile of Genes Modulated by Aloe emodin in Human U87 Glioblastoma Cells

Haris

Ismail²,

Idris³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Glioblastoma, the most aggressive and malignant form of glioma, appears to be resistant to various chemotherapeutic agents. Hence, approaches have been intensively investigated to targeti specific molecular pathways involved in glioblastoma development and progression. Aloe emodin is believed to modulate the expression of several genes in cancer cells. We aimed to understand the molecular mechanisms underlying the therapeutic effect of Aloe emodin on gene expression profiles in the human U87 glioblastoma cell line utilizing microarray technology. The gene expression analysis revealed that a total of 8,226 gene alterations out of 28,869 genes were detected after treatment with 58.6 µg/ml for 24 hours. Out of this total, 34 genes demonstrated statistically significant change (p<0.05) ranging from 1.07 to 1.87 fold. The results revealed that 22 genes were up-regulated and 12 genes were down-regulated in response to Aloe emodin treatment. These genes were then grouped into several clusters based on their biological functions, revealing induction of expression of genes involved in apoptosis (programmed cell death) and tissue remodelling in U87 cells (p<0.01). Several genes with significant changes of the expression level e.g. SHARPIN, BCAP31, FIS1, RAC1 and TGM2 from the apoptotic cluster were confirmed by quantitative real-time PCR (qRT-PCR). These results could serve as guidance for further studies in order to discover molecular targets for the cancer therapy based on Aloe emodin treatment.

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Section: Discussionsupporting

confidence: 94%

Expression Profile of Genes Modulated by Aloe emodin in Human U87 Glioblastoma Cells

Haris

Ismail²,

Idris³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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“…Aloe-emodin was found to cause cytochrome c release from mitochondria (21). In addition, it was observed that aloe-emodin-induced apoptosis in human hepatocellular carcinoma cell lines HepG2 and Hep3B was accompanied by the induction of p53 and p21 expression (5). Another report suggests that the decrease in the expression of protein kinase Cδ and ε isoforms plays a critical role in aloe-emodin-induced apoptosis (21).…”

Section: Discussionmentioning

confidence: 99%

“…Some studies have found that aloe-emodin has numerous biological properties including antiviral, antimicrobial and hepatoprotective activities (2). Aloe-emodin has been reported to exhibit anticancer activity on neuroectodermal tumors, lung squamous cell carcinoma and hepatoma cells (3)(4)(5). Aloe-emodin has also been shown to inhibit S-phase progression in both a transformed glia and a human glioma cell line, sensitize HeLa cells to As 2 O 3 via the generation of reactive oxygen species, and affect the anticancer activity of cisplatin by blocking the activation of extracellular signalregulated kinase (6)(7)(8).…”

Section: Introductionmentioning

confidence: 99%

Aloe-emodin, an anthraquinone, in vitro inhibits proliferation and induces apoptosis in human colon carcinoma cells

Lin

Uen²

2010

Oncology Letters

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Abstract. The present study aimed to investigate the anticancer effect of aloe-emodin, an anthraquinone compound present in the leaves of Aloe vera, on two human colon carcinoma cell lines, DLD-1 and WiDr. Colon carcinoma cells were treated with various concentrations of aloe-emodin for different durations. Cell viability was measured by sodium 3'-[1-(phenylamino-carbonyl)-3,4-tetrazolium]-bis(4-methoxy-6-nitro)benzene-sulfonic acid hydrate assay. DNA fragmentation was analyzed by agarose gel electrophoresis. Nuclear shrinkage was visualized by Hoechst 33258 staining. Western blotting was used to indicate the release of apoptosisinducing factor and cytochrome c from mitochondria and the phosphorylation of Bid. Caspase-3 and casein kinase II activities were measured by the respective assays. Cell viability analyses showed that aloe-emodin induced cell death in a dose-and time-dependent manner. Notably, the WiDr cells were more sensitive to aloe-emodin than the DLD-1 cells. Aloe-emodin caused the release of apoptosis-inducing factor and cytochrome c from mitochondria, followed by activation of caspase-3 leading to DNA fragmentation, nuclear shrinkage and apoptosis. In addition, exposure of colon carcinoma cells to aloe-emodin suppressed the casein kinase II activity in a time-dependent manner and was accompanied by a reduced phosphorylation of Bid, a downstream substrate of casein kinase II and a pro-apoptotic molecule. These findings showed that the inhibition of casein kinase II activity, the release of apoptosis-inducing factor and cytochrome c, and the caspase-3 activation are involved in aloe-emodin-mediated apoptosis in colon carcinoma cells.

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“…[22] In a laboratory study on hepatocellular cancer cell lines, it also inhibited cell proliferation and induced cell apoptosis. [23] In a study on breast cancer cell lines, it once again suppressed proliferation, [24] and in another study on colon cancer cell lines, it induced apoptosis through the activation of caspase-6. [25] Similarly, there are also studies showing that Aloe emodin inhibited cell growth in cell lines of lung cancer, leukemia, and neuroectodermal cancer.…”

Section: Laboratory Studies On the Effects Of Aloe Vera On Cancermentioning

confidence: 98%

Aloe Vera and Cancer

Ünlü¹

2016

TJ Oncol

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SUMMARYAloe vera, a succulent plant species, has a long history in folk medicine. Its clear, viscous liquid has been used to treat skin problems and other disorders since ancient times. In the last century, oral consumption and the injection of aloe have also come to popular attention. Its topical use is effective in the treatment of burns and abrasions, and oral use is effective in the treatment of constipation. However, it has not been found to be superior to standard treatments. Most recently, claims of anti-cancer properties are prevalent. It has been found to inhibit proliferation and angiogenesis, and to induce apoptosis in cancer cells. Yet other clinical studies indicate that Aloe vera did not prevent or reduce the number of radiotherapy-related lesions; it merely delayed onset. Furthermore, many instances of toxicity and mortality have been reported in the literature. Today, it is better to avoid it, especially forms taken orally or by injection.

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The antiproliferative activity of aloe-emodin is through p53-dependent and p21-dependent apoptotic pathway in human hepatoma cell lines

Cited by 172 publications

References 32 publications

Expression Profile of Genes Modulated by Aloe emodin in Human U87 Glioblastoma Cells

Expression Profile of Genes Modulated by Aloe emodin in Human U87 Glioblastoma Cells

Aloe-emodin, an anthraquinone, in vitro inhibits proliferation and induces apoptosis in human colon carcinoma cells

Aloe Vera and Cancer

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