The Antitumor Activity ofAntrodia camphoratain Melanoma Cells: Modulation of Wnt/β-Catenin Signaling Pathways

Abstract

Antrodia camphorata (AC) is well known in Taiwan as a traditional Chinese medicine. The aim of this study was to investigate whether a fermented culture broth of AC could inhibit melanoma proliferation and progression via suppression of the Wnt/β-catenin signaling pathway. In this study, we observed that AC treatment resulted in decreased cell viability and disturbed Wnt/β-catenin cascade in B16F10 and/or B16F1 melanoma cells. This result was accompanied by a decrease in the expression of Wnt/β-catenin transcriptional targets, including c-Myc and survivin. Furthermore, treatment of melanoma cells with AC resulted in a significant increase in apoptosis, which was associated with DNA fragmentation, cytochrome c release, caspase-9 and -3 activation, PARP degradation, Bcl-2/Bax dysregulation, and p53 expression. We also observed that AC caused G1 phase arrest mediated by a downregulation of cyclin D1 and CDK4 and increased p21 and p27 expression. In addition, we demonstrated that non- and subcytotoxic concentrations of AC markedly inhibited migration and invasion of highly metastatic B16F10 cells. The antimetastatic effect of AC was further confirmed by reductions in the levels of MMP-2, MMP-9, and VEGF expression. These results suggest that Antrodia camphorata may exert antitumor activity by downregulating the Wnt/β-catenin pathways.

MiR-27a modulates the MDR1/P-glycoprotein expression by inhibiting FZD7/β-catenin pathway in hepatocellular carcinoma cells

“…Hence, disruption of Wnt/β-catenin signaling represents an opportunity for rational cancer chemoprevention and therapy[50].In agreement with this current work, our results showed that miR27a, FZD7 and Wnt/β-catenin certainly constitute a regulatory circuit that may be disrupted in HCC cells. Firstly, miR-27a alteration reduced the expressions of FZD7, β-catenin and MDR1/P-gp.…”

“…However, multiple drug resistance (MDR) 46 constitutes a dominating clinical obstacle to successful treatment in HCC patients [2]. One of the common forms of MDR is caused by activa- 48 tion of the MDR1 gene, resulting in overexpression of P-glycoprotein 49 (P-gp) and conferring cancer cell resistance to a broad range of struc- 50 turally and functionally diverse chemotherapeutic drugs [3,4]. But so ation, and apoptosis [5,6].…”

The Extract of Rhodobacter sphaeroides Inhibits Melanogenesis through the MEK/ERK Signaling Pathway

“…Lycogen™ are available from Asia-Pacific Biotech Developing, Inc. (Kaohsiung, Taiwan). Murine B16F10 cells are cultured in Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum, 1% glutamine and 50 μg/mL gentamicin at 37 °C in 5% CO 2 [11,12]. Adult zebrafish were obtained from a commercial dealer, and 10–15 fishes were kept in a 5 L acrylic tank with the following conditions; 28.5 °C, with a 14/10 h light/dark cycle.…”

Toxicity study in blood and tumor cells of laser produced medicines for application in fabrics

“…From one side, the fabric itself hosts the bacteria acting as a medium between the human organism and the microorganisms; on the other hand, it is regarded as a well assessed tool for drug delivery in contact of skin for external applications [24][25][26]. Therefore wide market sectors push textile industries to concentrate more on antibacterial fabrics manufacturing to improve fabrics quality and product sales.…”