The antitumor activity of the hydrolysates of chitinous materials hydrolyzed by crude enzyme from Bacillus amyloliquefaciens V656

Liang, Tzu Wen; Chen, Yu-Jen; Yen, Yue Horng; Wang, San-Lang

doi:10.1016/j.procbio.2006.10.005

Cited by 93 publications

(61 citation statements)

References 19 publications

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“…In our study, the fractional factorial was used for the first time in screening design, and followed by CCD to utilize optimum medium component for the production of chitinase and gelatinase by Lysobacter capsici YS1215. We used crab shell powder and gelatin to optimize the production of chitinase and gelatinase by L. capsici YS1215 because they are obviously simpler and cheaper than the chitin powder and other expensive nutrients (Wang et al, 2006;Liang et al, 2007). In conclusion, the use of the statistically based designs has aided a lot in finding out the medium components, which are effective on the production of these enzymes that can be applied as biological control agents on agricultural fungal pathogens.…”

Section: Resultsmentioning

confidence: 99%

Optimization of Medium Components for the Production of Antagonistic Lytic Enzymes Against Phytopathogenic Fungi and Their Biocontrol Potential

Lee¹,

Neung²,

Park³

et al. 2014

Korean Journal of Soil Science and Fertilizer

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In this paper, fractional factorial screening design (FFSD) and central composition design (CCD) were used to optimize the medium components for producing chitinase and gelatinase by Lysobacter capsici YS1215. Crab shell powder, nutrient broth and gelatin were proved to have significant effects on chitinase and gelatinase activity by FFSD first. An optimal medium was obtained by using a three factor CCD, which consisted of nutrient broth of 2.0 g L ). This value was 3.76 and 1.11 fold of the chitinase and gelatinase activity, respectively, compared to the lowest productive medium in the design matrix. In investigating potential of these enzymes partially purified from L. capsici YS1215 for biotechnological use, the crude enzymes was found to be inhibition against pathogenic fungal mycelia: Colletotrichum gleosporioides, Phytophthora capsici, and Rhizoctonia solani. In this study, we demonstrated the optimal medium for producing the chitinolytic and gelatinolytic enzymes by the strain YS1215 and the role of their enzymes that may be useful for further development of a biotechnological use and agricultural use for biological control of phytopathogenic fungi.

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Section: Resultsmentioning

confidence: 99%

Optimization of Medium Components for the Production of Antagonistic Lytic Enzymes Against Phytopathogenic Fungi and Their Biocontrol Potential

Lee¹,

Neung²,

Park³

et al. 2014

Korean Journal of Soil Science and Fertilizer

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“…Li X, Wang J, Chen X J, et al [6,7]. The antitumor activity of COS is likely influenced by its chemical structure, molecular size, and strong electric charge [6][7][8].…”

Section: Citationmentioning

confidence: 99%

“…The antitumor activity of COS is likely influenced by its chemical structure, molecular size, and strong electric charge [6][7][8]. Administration of N-acetylchitohexaose (NACOS-6) resulted in a significant increase in the candidacidal activity of macrophages and T lymphocytes in the early phase of tumor development [8] and COS was suggested to play a role in the induction of apoptosis of carci-*Corresponding author (email: tcrz9@jnu.edu.cn) noma cells via up-regulation of Bax [9].…”

Section: Citationmentioning

confidence: 99%

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Effect of chitooligosaccharides on cyclin D1, bcl-xl and bcl-2 mRNA expression in A549 cells using quantitative PCR

Wang

Chen

et al. 2011

Chin. Sci. Bull.

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Chitooligosaccharide (COS) is derived from the chemical and enzymatic hydrolysis of chitosan and has been reported to have potent antitumor activity. Our study investigated the effects of five chitooligomers ranging from the dimer form to the hexamer form (chitobiose, chitotriose, chitotetraose, chitopentaose, chitohexaose) on the expression of cyclin D1, bcl-2 and bcl-xl mRNA in A549 cells using reverse transcription quantitative real-time PCR. We demonstrated that, of the five chitooligomers used, chitohexaose (COS6) had the most potent inhibitory effect on A549 cell proliferation. COS6 also significantly down regulated cyclin D1 and bcl-xl mRNA expression levels. Our data suggested that COS6 exerts its antitumor activity by two different mechanisms: (1) COS6-mediated inhibition of Cyclin D1 levels leads to suppression of tumor cell proliferation; and (2) COS6-mediated down-regulation of the pro-survival protein, Bcl-xl, promotes the apoptosis of tumor cells. chitooligosaccharides, cyclin D1, bcl-xl, bcl-2 Citation:Li X, Wang J, Chen X J, et al. [6,7]. The antitumor activity of COS is likely influenced by its chemical structure, molecular size, and strong electric charge [6][7][8]. Administration of N-acetylchitohexaose (NACOS-6) resulted in a significant increase in the candidacidal activity of macrophages and T lymphocytes in the early phase of tumor development [8] and COS was suggested to play a role in the induction of apoptosis of carci-*Corresponding author (email: tcrz9@jnu.edu.cn) noma cells via up-regulation of Bax [9]. The chitohexamer form was shown to down-regulate VEGF and uPA mRNA expression levels in ECV304 cells [10], and to inhibit tumor growth and metastasis by up-regulation of p21 and MMP-9, and down-regulation of PCNA, cyclin A and cdk-2 [11]. However, the effects of different forms of COS on the expression levels of cyclin D1 and the pro-survival proteins bcl-2 and bcl-xl are unknown. In this study, we investigated the role of five different fractions [chitobiose (COS2), chitotriose (COS3), chitotetraose (COS4), chitopentaose (COS5), chitohexaose (COS6)] on the regulation of cyclin D1, bcl-2 and bcl-xl mRNA levels in A549 cells. We demonstrated that COS6 exhibited the most potent inhibitory activity on A549 cell proliferation by downregulation of cyclin D1 mRNA. Treatment of A549 cells with COS6 also resulted in suppression of bcl-xl mRNA expression levels.

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“…and anticarcinogenic (Liang et al, 2007;Wang et al, 2008b) properties. To increase the utilization of the chitin/protein-containing marine wastes, we incubated TKU010 and TKU012 for 1 4 days with squid pen powder under the optimal culture conditions of extracellular protease production (Wang et al, 2008a, b) and analyzed the antioxidant activity and protease activity of the culture supernatant.…”

Section: Antioxidant Property Of the Culture Supernatantmentioning

confidence: 99%

Conversion of squid pen by a novel strain Lactobacillus paracasei subsp. paracasei TKU010, and its application in antimicrobial and antioxidants activity

Liang

Huang

et al. 2010

J. Gen. Appl. Microbiol.

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TKU010 was isolated from infant vomited milk and identifi ed as Lactobacillus paracasei subsp. paracasei. TKU010 had desirable properties concerning its ability to withstand adverse conditions in the gastrointestinal tract. The hydrolysate of casein enhanced the growth of TKU010 most obviously (17.20 18.25 OD 660 ), followed by the hydrolysate of SPP (16.00 15.06 OD 660 ). Incubating with SPP, both the culture supernatant of TKU010 on the fi rst day and the fourth day showed inhibitory activities on E. coli BCRC13086, F. oxysporum BCRC32121 and A. fumigatus BCRC30099. TKU010 culture supernatant (1% SPP) incubated for 3 days has high antioxidant activity; the DPPH scavenging ability was 75% per ml. Thus, TKU010 could be preferably used as a starter to produce fermented milk with possibly interesting organoleptic properties. Besides, we have shown that squid pen wastes can be utilized to generate a high value-added product, and have revealed its hidden potential in the production of biocontrol agents and functional foods.

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The antitumor activity of the hydrolysates of chitinous materials hydrolyzed by crude enzyme from Bacillus amyloliquefaciens V656

Cited by 93 publications

References 19 publications

Optimization of Medium Components for the Production of Antagonistic Lytic Enzymes Against Phytopathogenic Fungi and Their Biocontrol Potential

Optimization of Medium Components for the Production of Antagonistic Lytic Enzymes Against Phytopathogenic Fungi and Their Biocontrol Potential

Effect of chitooligosaccharides on cyclin D1, bcl-xl and bcl-2 mRNA expression in A549 cells using quantitative PCR

Conversion of squid pen by a novel strain Lactobacillus paracasei subsp. paracasei TKU010, and its application in antimicrobial and antioxidants activity

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