The antitumor and antimetastatic effects of N-trimethyl chitosan-encapsulated camptothecin on ovarian cancer with minimal side effects

Zhao, Xiujian

doi:10.3892/or.2010.941

Cited by 13 publications

(6 citation statements)

References 16 publications

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“…The nude mice were randomly assigned to three groups (six mice/group). WT-SK, EV-SK, VEGFD-SK cells were injected subcutaneously with 2×10 6 cells into the left hind paws of mice respectively ( 25 ). The tumor volume was determined by the following formula: tumor volume (mm 3 ) = 0.52 × length (mm) × width (mm) × height (mm).…”

Section: Methodsmentioning

confidence: 99%

VEGF-D-enhanced lymph node metastasis of ovarian cancer is reversed by vesicular stomatitis virus matrix protein

Chen

et al. 2016

International Journal of Oncology

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Lymphatic metastasis is a poor prognostic factor in ovarian cancer, which correlates to the majority of cancer deaths. Matrix protein (MP) of vesicular stomatitis virus (VSV) exhibits potent antitumor and antiangiogenic activities through inducing apoptosis and inhibiting angiogenesis. In this study, the antitumor and antimetastatic effects of MP were further investigated. Wild-type SKOV3 (WT-SK) cells were successfully transfected with empty vector pcDNA3.1 plasmid, or pcDNA3.1-VEGF-D recombinant plasmid to construct cell lines named EV-SK, and VEGFD-SK, respectively. Inhibition of VEGFD-SK cell migration and invasion was detected by Transwell and wound healing assay. Then, lymphogenous metastatic model of ovarian cancer was established by injecting VEGFD-SK cells subcutaneously into the left hindlimb claw pad of nude mice. The inducted apoptotic effect of MP on VEGFD-SK cells were assessed by flow analysis and Hoechst-33258 staining, respectively, in vitro. The in vivo antitumor and antiangiogenic activities of MP gene were evaluated with lymphogenous metastatic model of ovarian cancer. Tumor volume and lymphatic metastasis rates were measured. Lymphatic vessels were delineated using Evan's blue and LYVE-1 staining. Expression of VEGF-D and MMP-2 were evaluated by immunostaining. Apoptosis of tumor cells was analyzed by Hoechst-33258 staining. Mice bearing VEGFD-SK tumor cells displayed more rapid tumorigenesis, higher lymphogenous metastatic tendency and increased lymphatic vessel density compared with the mice bearing WT-SK or EV-SK cells. However, VEGF-D-enhanced metastasis was evidently reversed by MP. MP significantly reduced the invasion of VEGFD-SK cells, tumor volume, lymphatic metastasis rates and lymphatic vessel density compared with control groups (P<0.05), accompanied with down-expression of VEGF-D and MMP-2 and increased apoptosis. Our data indicate that MP has strong antitumor and antimetastatic abilities, and it may be a promising therapeutic strategy against the lymphatic metastasis of human ovarian cancer.

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Section: Methodsmentioning

confidence: 99%

VEGF-D-enhanced lymph node metastasis of ovarian cancer is reversed by vesicular stomatitis virus matrix protein

Chen

et al. 2016

International Journal of Oncology

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“…From the studies reviewed it is concluded that Chitosan and Chitosan derivatives are promising polymeric excipients for mucosal drug and vaccine derivatives [65,66,68,69,71]. This absorption enhancing effect of Chitosan is caused by opening of the intercellular tight junctions, thereby favouring the paracellular transport of macromolecular drugs.…”

Section: Chitosan and Its Derivatives In Mucosal Drug And Vaccine Delmentioning

confidence: 99%

Naturally Occurring Chitosan and Chitosan Derivatives: A Review

Yadav¹,

Joshi²,

Singh³

et al. 2011

CDTH

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Chitosan, a polymer obtained by deacetylation of chitin is widely studied for its pharmaceutical and nonpharmaceutical applications. Recommendations about uses of this polymer although could not be always realized due to limited solubility. Chitosan has been extensively evaluated for its mucoadhesive and absorption enhancement properties. The positive charge on the chitosan molecule gained by acidic environment in which it is soluble seems to be important for absorption enhancement. However chitosan is not soluble in medium except below pH 5.6. This limits its use as permeation enhancer in body compartments where pH is high. In this regard there is a need for chitosan derivatives with increased solubility, especially at neutral and basic pH values. This review introduces the physical background of the special properties of the various chitosan derivatives and their application. Chitosan derivatives with different physicochemical properties especially water solubility at neutral and basic values are of interest. Chemical modification of chitosan is useful for the association of bioactive molecules to polymerize and controll the drug release profile. From the studies reviewed it is concluded that chitosan derivatives are promising materials for controlled drug and nonviral gene delivery.

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“…As a traditional herbal medicine, CPP’s cardiotonic and diuretic activity have been well recognized [28]. Recent studies have shown that CPP can inhibit the proliferation and promote the apoptosis in several cancer cells [29–31]. Previously we have shown that CPP has the anti-tumor activity in gastric cancer and colon cancer [29, 31], partly through inhibition of the Wnt/β-catenin pathway [29, 32].…”

Section: Introductionmentioning

confidence: 99%

“…Recent studies have shown that CPP can inhibit the proliferation and promote the apoptosis in several cancer cells [29–31]. Previously we have shown that CPP has the anti-tumor activity in gastric cancer and colon cancer [29, 31], partly through inhibition of the Wnt/β-catenin pathway [29, 32]. In other studies, CPP was found to induce the expression of DRs and enhance TRAIL-induced apoptosis in hepatocellular carcinoma cells that were resistant to TRAIL, while the mechanism of which is still unclear until now [33].…”

Section: Introductionmentioning

confidence: 99%

Combination of the natural compound Periplocin and TRAIL induce esophageal squamous cell carcinoma apoptosis in vitro and in vivo: Implication in anticancer therapy

Han

Dai

et al. 2019

J Exp Clin Cancer Res

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BackgroundEsophageal cancer is one of the most common malignant tumors in the world. With currently available therapies, only 20% ~ 30% patients can survive this disease for more than 5 years. TRAIL, a natural ligand for death receptors that can induce the apoptosis of cancer cells, has been explored as a therapeutic agent for cancers, but it has been reported that many cancer cells are resistant to TRAIL, limiting the potential clinical use of TRAIL as a cancer therapy. Meanwhile, Periplocin (CPP), a natural compound from dry root of Periploca sepium Bge, has been studied for its anti-cancer activity in a variety of cancers. It is not clear whether CPP and TRAIL can have activity on esophageal squamous cell carcinoma (ESCC) cells, or whether the combination of these two agents can have synergistic activity.MethodsWe used MTS assay, flow cytometry and TUNEL assay to detect the effects of CPP alone or in combination with TRAIL on ESCC cells. The mechanism of CPP enhances the activity of TRAIL was analyzed by western blot, dual luciferase reporter gene assay and chromatin immunoprecipitation (ChIP) assay. The anti-tumor effects and the potential toxic side effects of CPP alone or in combination with TRAIL were also evaluated in vivo.ResultsIn our studies, we found that CPP alone or in combination with TRAIL could inhibit the proliferation of ESCC cells and induce apoptosis, and we certificated that combination of two agents exert synergized functions. For the first time, we identified FoxP3 as a key transcriptional repressor for both DR4 and DR5. By down-regulating FoxP3, CPP increases the expression of DR4/DR5 and renders ESCC cells much more sensitive to TRAIL. We also showed that CPP reduced the expression of Survivin by inhibiting the activity of Wnt/β-catenin pathway. All these contributed to synergistic activity of CPP and TRAIL on ESCC cells in vitro and in vivo.ConclusionOur data suggest that CPP and TRAIL could be further explored as potential therapeutic approach for esophageal cancer.

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The antitumor and antimetastatic effects of N-trimethyl chitosan-encapsulated camptothecin on ovarian cancer with minimal side effects

Cited by 13 publications

References 16 publications

VEGF-D-enhanced lymph node metastasis of ovarian cancer is reversed by vesicular stomatitis virus matrix protein

VEGF-D-enhanced lymph node metastasis of ovarian cancer is reversed by vesicular stomatitis virus matrix protein

Naturally Occurring Chitosan and Chitosan Derivatives: A Review

Combination of the natural compound Periplocin and TRAIL induce esophageal squamous cell carcinoma apoptosis in vitro and in vivo: Implication in anticancer therapy

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