The antiulcer effect of <em>Cibotium barometz</em> leaves in rats with experimentally induced acute gastric ulcer

Abstract

Cibotium barometz is a pharmaceutical plant customarily used in traditional medicine in Malaysia for the treatment of different diseases, such as gastric ulcer. The gastroprotective effect of C. barometz leaves against ethanol-induced gastric hemorrhagic abrasions in Sprague Dawley rats has been evaluated in terms of medicinal properties. Seven groups of rats (normal control and ulcerated control groups, omeprazole 20 mg/kg, 62.5, 125, 250, and 500 mg/kg of C. barometz correspondingly) were used in antiulcer experiment and pretreated with 10% Tween 20. After 1 hour, the normal group was orally administered 10% Tween 20, whereas absolute alcohol was fed orally to ulcerated control, omeprazole, and experimental groups. Gastric’s homogenate were assessed for endogenous enzymes activities. Stomachs were examined macroscopically and histologically. Grossly, the data demonstrated a significant decrease in the ulcer area of rats pretreated with plant extract in a dose-dependent manner with respect to the ulcerated group. Homogenates of the gastric tissue exhibited significantly increased endogenous enzymes activities in rats pretreated with C. barometz extract associated with the ulcerated control group. Histology of rats pretreated with C. barometz extract group using hematoxylin and eosin staining exhibited a moderate-to-mild disruption of the surface epithelium with reduction in submucosal edema and leucocyte infiltration in a dose-dependent manner. In addition, it showed heat shock protein70 protein up-expression and BCL2-associated X protein downexpression. These outcomes might be attributed to the gastroprotective and antioxidative effects of the plant.

Gamma (γ)-mangostin attenuated gastric ulcers induced by absolute alcohol in rats: Histological, immunohistochemical and biochemical investigation

“…This outcome led us to hypothesize that the up-regulation of HSP70 protein synthesis might play an essential role in promoting the gastroprotective effect of γ-mangostin, possibly by attenuating ROS-mediated gastric oxidative stress. Similarly, numerous studies demonstrate that the upregulation of HSP70 in experimental animals protect stomach from necrotizing agents (Halabi et al 2014;Nazarbahjat et al 2016;AL-Wajeeh et al 2017). The results of the current study revealed that stomach tissue homogenates from experimental groups showed expressively reduced levels of MDA and elevated levels of SOD and CAT in retort to oxidative stress induced by absolute ethanol.…”

Antiulcer Agents: From Plant Extracts to Phytochemicals in Healing Promotion

“…Cibotium barometz (L.) J. Smith is a tropical and sub-tropical plant, also known as “golden hair dog fern,” used as medicinal plant in Malaysian Peninsula and parts of China. The gastroprotective effect of the ethanolic extract from its leaves [ 53 ] and hairs [ 54 ] was evaluated against ethanol-induced stomach ulcers in Sprague Dawley rats at 62.5, 125, 250 and 500 mg/kg. The C. barometz leaves presented a dose-dependent antiulcer effect, increased antioxidant enzymes activity, such as superoxide dismutase (SOD), CAT and glutathione peroxidase (GPx) and decreased lipid peroxidation [ 53 ].…”

“…The gastroprotective effect of the ethanolic extract from its leaves [ 53 ] and hairs [ 54 ] was evaluated against ethanol-induced stomach ulcers in Sprague Dawley rats at 62.5, 125, 250 and 500 mg/kg. The C. barometz leaves presented a dose-dependent antiulcer effect, increased antioxidant enzymes activity, such as superoxide dismutase (SOD), CAT and glutathione peroxidase (GPx) and decreased lipid peroxidation [ 53 ]. On the other hand, C. barometz hairs presented a dose-dependent antiulcer effect too, free radical scavenging activities, increased pH, gastric mucus glycoprotein and antioxidant enzymes activity (SOD, CAT and GPx) and reduced MDA levels [ 54 ].…”

“…On the other hand, C. barometz hairs presented a dose-dependent antiulcer effect too, free radical scavenging activities, increased pH, gastric mucus glycoprotein and antioxidant enzymes activity (SOD, CAT and GPx) and reduced MDA levels [ 54 ]. Moreover, the effects of both C. barometz leaves and hairs were proposed as being related to HSP70 up-regulation and Bax protein down-regulation [ 53 , 54 ].…”

Herbal Medicines in the Treatment of Dyspepsia: An Overview

“…This effect was attributed to increased antioxidant activity, mucus preservation, upregulation of HSP-70, and downregulation of Bax proteins [51]. Similarly, C. barometz leaves also demonstrated a gastroprotective effect against ethanol-induced gastric ulcers in rats, an effect attributed to upregulated HSP-70 expression and downregulation of Bcl-2 protein expression [52].…”