The antiviral effect of mollusk mucus on measles virus

Toledo-Piza, Ana Rita de; Figueiredo, Cristina; Oliveira, Maria Isabel de; Negri, Giuseppina; Namiyama, Gislene Mitsue; Tonelotto, Mariana; Villar, Karina de Senna; Rofatto, Henrique K.; Mendonça, Ronaldo Zucatelli

doi:10.1016/j.antiviral.2016.09.005

Cited by 8 publications

(7 citation statements)

References 42 publications

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“…Recent studies have demonstrated the importance of antiviral compounds from marine invertebrate; such as Phyllocaulis boraceiensis, which contains polyunsaturated fatty acids that disturb the virus envelope (Toledo-Piza et al, 2016). Manzamine A, from the sponge genus Acanthostrongylophora, works by inhibiting viral replication (Palem et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Bioactivity of compounds secreted by symbiont bacteria of Nudibranchs from Indonesia

Kristiana

Bedoux

Pals

et al. 2020

PeerJ

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The aims of this work are to isolate bacterial symbionts from nudibranchs and subsequently to determine anti-Methicillin resistant Staphylococcus aureus (MRSA), cytotoxicity and anti-Herpes simplex virus type 1 (HSV-1) activities of bio compounds. A total of 15 species of nudibranchs were collected from Karimunjawa and five species from Bali, respectively. A total of 245 bacteria isolates were obtained. The anti-MRSA activity screening activity indicated two active bacteria. Ethyl acetate extracts from supernatants, indicating extracelullar compounds, showed an inhibition zone against MRSA at concentrations of 500-1,000 µg/ml. DNA sequence analysis showed that the strain KJB-07 from Phyllidia coelestis was closely related to Pseudoalteromonas rubra, whereas the strain NP31-01 isolated from Phyllidia varicosa was closely related to Virgibacillus salarius. The extract of Pseudoalteromonas rubra was cytotoxic to Vero cells at a concentration of 75 µg/ml. The extract of V. salarius presented no cytotoxicity at concentrations of 5-1,000 µg/ml. No anti HSV-1 was observed for both isolated bacteria. This is the first study describing research on anti-MRSA, cytotoxicity and anti HSV-1 activity of bacterial symbionts from the viscera of nudibranch. Compounds produced by Pseudoalteromonas rubra and V. salarius, had potential anti-MRSA activity. However, only extracts from Pseudoalteromonas rubra showed cytotoxic effects on Vero cells. Three compounds were identified by LC/MS after purification from culture supernatant. activity of marine bacteria associated with sponges from the waters off the coast of South East India. Microbiological Research 161 (3):252-262 DOI 10.1016/j.micres.2005.09.002. Avila C. 2006. Molluscan natural products as biological models: chemical ecology, histology, and laboratory culture. Chand S, Karuso P. 2017. Isolation and total synthesis of two novel metabolites from the fissurellid mollusc Scutus antipodes. Tetrahedron Letters 58(10):P. 1992. A one-step microbial DNA extraction method using "Chelex 100" suitable for gene amplification. Research in Microbiology 143(8):785-790

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Section: Discussionmentioning

confidence: 99%

Bioactivity of compounds secreted by symbiont bacteria of Nudibranchs from Indonesia

Kristiana

Bedoux

Pals

et al. 2020

PeerJ

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“…The antiviral action could be correlated to polyunsaturated fatty acids in fraction 39, in detail the most active fatty acids against MV were hydroxy-tritriacontapentaenoic acid and hydroxy-pentatriacontapentaenoic acid. Furthermore, the fraction 50 showed a lower antiviral activity against tested virus [44].…”

Section: Antimicrobial Activitymentioning

confidence: 94%

“…The fraction 39 and fraction 50 from Phyllocaulis boraceiensis, tropical leatherleaf slug, mucus was analyzed using Fourier Transform Infrared Spectrometry (FT-IR) and tested against Measles virus (MV), a singlestranded, negative-sense, enveloped RNA virus of the genus Morbillivirus [44]. The antiviral action could be correlated to polyunsaturated fatty acids in fraction 39, in detail the most active fatty acids against MV were hydroxy-tritriacontapentaenoic acid and hydroxy-pentatriacontapentaenoic acid.…”

Section: Antimicrobial Activitymentioning

confidence: 99%

Antimicrobial properties of terrestrial snail and slug mucus

Cilia

Fratini

2018

Journal of Complementary and Integrative Medicine

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Snail and slug mucus is a viscous-elastic substance secreted by specific glands with adhesive and lubricants properties that allows them to adhere tenaciously to many different surfaces. It has been used since ancient times for care and human health and it is still very important in traditional and folkloristic medicine. Recently, mucus from snail and slugs and its protein and components have been subjected to some investigations on their antibacterial, antiviral and antifungal activity due to extensive traditional uses and for a future application in medicine. Antimicrobial activities of crude mucus, and its components, against different microorganism have been reported, showing antimicrobial activities that lead their potential employment in several fields as natural additives. The purpose of this Review is to summarize the results of antimicrobial studies of snail and slug mucus and its compounds from the first scientific applications to the isolation of the single components in order to better understand its application and propose an employment in future studies as a natural antimicrobial agent.

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“…By comparison, biomedical research efforts have been focused in Europe (34%), North America (28%), and Asia (China and India, 27%). Only one South American species has been studied [153,154] and Australian species are also underrepresented (Table S1). Numerous sources attest to the importance of molluscs as traditional food sources as opposed to traditional medicines in Australia and throughout the Indo-pacific [155,156].…”

Section: Taxonomic and Geographic Trendsmentioning

confidence: 99%

“…Preliminary antibacterial results from disc diffusion assays using some respiratory pathogens are presented by Cilia and Fratini [189], but statistically meaningful data are yet to be derived. de Toledo-Piza et al (2016) demonstrated impressive anti-viral activity with concentrated mucus from the terrestrial shell-less mollusc Phyllocaulis boraceiensis whereby host cell pre-treatment reduced Influenza A (H1N1) and measles virus-induced cytopathic effects by up to 80% with no host cell cytotoxicity [153,154]. Further studies should incorporate bioassay guided fractionation of concentrated mucus extracts to isolate and identify the active factors.…”

Section: Antimicrobial Activitymentioning

confidence: 99%

Molluscan Compounds Provide Drug Leads for the Treatment and Prevention of Respiratory Disease

et al. 2020

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Respiratory diseases place an immense burden on global health and there is a compelling need for the discovery of new compounds for therapeutic development. Here, we identify research priorities by critically reviewing pre-clinical and clinical studies using extracts and compounds derived from molluscs, as well as traditional molluscan medicines, used in the treatment of respiratory diseases. We reviewed 97 biomedical articles demonstrating the anti-inflammatory, antimicrobial, anticancer, and immunomodulatory properties of >320 molluscan extracts/compounds with direct relevance to respiratory disease, in addition to others with promising bioactivities yet to be tested in the respiratory context. Of pertinent interest are compounds demonstrating biofilm inhibition/disruption and antiviral activity, as well as synergism with approved antimicrobial and chemotherapeutic agents. At least 100 traditional medicines, incorporating over 300 different mollusc species, have been used to treat respiratory-related illness in cultures worldwide for thousands of years. These medicines provide useful clues for the discovery of bioactive components that likely underpin their continued use. There is particular incentive for investigations into anti-inflammatory compounds, given the extensive application of molluscan traditional medicines for symptoms of inflammation, and shells, which are the principal molluscan product used in these preparations. Overall, there is a need to target research toward specific respiratory disease-related hypotheses, purify bioactive compounds and elucidate their chemical structures, and develop an evidence base for the integration of quality-controlled traditional medicines.

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The antiviral effect of mollusk mucus on measles virus

Cited by 8 publications

References 42 publications

Bioactivity of compounds secreted by symbiont bacteria of Nudibranchs from Indonesia

Bioactivity of compounds secreted by symbiont bacteria of Nudibranchs from Indonesia

Antimicrobial properties of terrestrial snail and slug mucus

Molluscan Compounds Provide Drug Leads for the Treatment and Prevention of Respiratory Disease

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