The antiviral role of heat shock protein 27 against red spotted grouper nervous necrosis virus infection in sea perch

Le, Yi; Jia, Peng; Jin, Yilin; Liu, Wei; Yi, Meisheng

doi:10.1016/j.fsi.2017.08.032

Cited by 20 publications

(17 citation statements)

References 47 publications

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“…Further experiments would be necessary to determine the association of ISG15, ZNF395 and IKK signalling in sea perch. In addition, among these DEGs, HSPB1, also named HSP27, was down‐regulated, similar to our previous study (Le, Jia et al, ). HSPB1 has been reported to be widely involved in pathophysiology of oxidative stress and apoptosis.…”

Section: Discussionsupporting

confidence: 90%

“…Overexpression of HSPB1 protected cells from oxidative damage and apoptosis triggered by Cd exposure (Mun̄oz‐Fontela, ). In our previous study, overexpression of LjHSP27 inhibited RGNNV‐induced apoptosis, supporting the vital role of HSP27 as an anti‐apoptosis protein (Le, Jia et al, ). It has been known that many DNA and RNA viruses can trigger oxidative stress and induce host cell death in infected cells (Casavant et al, ; Yuan et al, ).…”

Section: Discussionsupporting

confidence: 68%

“…Several studies have reported that p53 can induce apoptosis through activating death receptor pathway or mitochondrial pathway (Benchimol, ; Moll & Zaika, ). Our previous study demonstrated that RGNNV infection caused apoptosis at 6 and 24 hpi in LJB cells (Le, Jia et al, ). Meanwhile, our results indicated that three apoptosis‐related genes, including Casp3 , Casp9 and CytC , were notably enhanced as the ectopic expression of Ljp53 in vitro, whereas pifithrin‐α caused the opposite effect.…”

Section: Discussionmentioning

confidence: 95%

“…In teleost fish, the innate immune system plays a critical role in host against virus infection, but studies on the immune system of sea perch are still limited. In recent years, researches on immunity of sea perch against NNV infection were mainly focused on single genes, and only a few immune‐related genes, including LGP2 (Jia, Zhang et al, ), MDA5 (Jia, Jia et al, ), MAVS (Jia, Jin et al, ), TRAF3 (Zhang et al, ), HSP27 (Le, Jia et al, ) and RAVER1 (Jia et al, ), have been identified and characterized in sea perch. However, knowledge about the immunity system of sea perch, especially the immune response‐related signalling pathways implicated in NNV infection, still remains incomplete.…”

Section: Introductionmentioning

confidence: 99%

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Comparative transcriptome analysis reveals the role of p53 signalling pathway during red‐spotted grouper nervous necrosis virus infection in Lateolabrax japonicus brain cells

Xiang

Jia

Liu

et al. 2019

Journal of Fish Diseases

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Nervous necrosis virus (NNV) is one of the fish pathogens that have caused mass mortalities of many marine and freshwater fishes in the world. To better comprehend the molecular immune mechanism of sea perch (Lateolabrax japonicus) against NNV infection, the comparative transcriptome analysis of red‐spotted grouper nervous necrosis virus (RGNNV)‐infected or mock‐infected L. japonicus brain (LJB) cells was performed via RNA sequencing technology. Here, 1,969 up‐regulated genes and 9,858 down‐regulated genes, which were widely implicated in immune response pathways, were identified. Furthermore, we confirmed that p53 signalling pathway was repressed at 48 hr post‐RGNNV infection, as indicated by up‐regulation of Mdm2 and down‐regulation of p53 and its downstream target genes, including Bax, Casp8 and CytC. Overexpression of L. japonicus p53 (Ljp53) significantly inhibited RGNNV replication and up‐regulated the expression of apoptosis‐related genes, whereas the down‐regulation caused by pifithrin‐α led to the opposite effect, suggesting Ljp53 might promote cell apoptosis to repress virus replication. Luciferase assay indicated that Ljp53 could enhance the promoter activities of zebrafish interferon (IFN)1, indicating that Ljp53 could exert its anti‐RGNNV activities by enforcing the type I IFN response. This study revealed the potential antiviral role of p53 during NNV infection.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 68%

Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Comparative transcriptome analysis reveals the role of p53 signalling pathway during red‐spotted grouper nervous necrosis virus infection in Lateolabrax japonicus brain cells

Xiang

Jia

Liu

et al. 2019

Journal of Fish Diseases

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“…Moreover, for viral diseases, it has been described that heat‐shock protein 27 can bind to cytochrome c and induce apoptosis after red‐spotted grouper nervous necrosis virus infection (Le et al . 2017). Interestingly, regarding this protein, the genes that encode cytochrome c were the most represented among the cytochromes DEGs ( n = 531) in the analysed studies.…”

Section: Resultsmentioning

confidence: 99%

Insights into gene expression responses to infections in teleosts using microarray data: a systematic review

Caruffo

Mandaković²,

Cabrera³

et al. 2020

Reviews in Aquaculture

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The rapid growth of production in aquaculture in the last decades has brought unwanted consequences affecting fish health and increasing the susceptibility to different infections. This systematic review aimed to analyse and summarize the current knowledge of gene expression responses to infectious diseases in teleosts using viruses, bacteria, fungi and parasites as agents through published microarray data. We conducted searches in electronic databases, including PubMed, Web of Science and SCOPUS until 1 May 2019. We identified 862 citations across databases and manual searches. After removing duplicates, we screened 455 unique references using titles and abstracts, of which 262 proved potentially eligible and evaluated using full text. A total of 79 articles proved eligible for this review. From the articles retrieved, we examined 261 different experiments (or 'studies') and more than a hundred thousand differentially expressed genes (DEGs). This systematic review represents the first catalogue of genes (and their associated processes) that differentially transcribe in different teleost species (13 species) due to infections generated by a large variety of pathogens (38 types). Although the obtained gene expression results are in considerable measure associated with expected immune response, other genes showed surprising significant transcriptional outcomes that may unravel unknown functions related to fish infections. This type of investigations facilitates the visualization of existing gaps in researches that may inspire future analysis in non-traditional but relevant host or pathogen species.

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Multiple isoforms of HSP70 and HSP90 required for betanodavirus multiplication in medaka cells

Zenke

Okinaka

2022

Arch Virol

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The antiviral role of heat shock protein 27 against red spotted grouper nervous necrosis virus infection in sea perch

Cited by 20 publications

References 47 publications

Comparative transcriptome analysis reveals the role of p53 signalling pathway during red‐spotted grouper nervous necrosis virus infection in Lateolabrax japonicus brain cells

Comparative transcriptome analysis reveals the role of p53 signalling pathway during red‐spotted grouper nervous necrosis virus infection in Lateolabrax japonicus brain cells

Insights into gene expression responses to infections in teleosts using microarray data: a systematic review

Multiple isoforms of HSP70 and HSP90 required for betanodavirus multiplication in medaka cells

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