The aplication of cyanoacrilate surgical glue on skin suture in rats

Abstract

Purpose: To compare the use of a new cyanoacrylate-based surgical glue and suture with separate points in skin wounds closure. Methods: Thirty-six rats were subjected to a 4cm dorsal longitudinal incision. Twelve were subjected to simple suture with polyamide 6-0, 12 rats underwent wall synthesis using Dermabond® and 12 was performed cutaneous synthesis with N-2-Butyl-Cyanoacrylate. Twelve of each group was euthanized on the seventh postoperative day, their blood was taken to biochemical tests and a layer of skin and subcutaneous tissue surrounding the surgical scar was randomly divided in two segments, to the submission of tension tests and to histological study. Results: There were no significant difference between groups (p>0.05). In the soft dermis there was more type I collagen production in group I (p<0.05), group II and III was similar re-sults (p>0.05). In the compact dermis, all 3 groups showed similar results (p>0.05). The biomechanical study was similarity between the glue groups (p>0.05) but the group III proved to be different from the others having a higher resistance (p>0.05). Conclusion: This glue does not cause any inflammation or kidney and hepatic toxicity. Polyamide sutures are more resistant and the glue should be used alone only in less tension are-as.

Use of cyanoacrylate-based surgical adhesives associated to the macroporous tape in skin synthesis in rats

“…It is worth noting that the methodology was similar to this experiment, with manual approximation and subsequent application of the adhesives. In this work, statistical equivalence was found between the groups where the adhesives were used in isolation (I and II), corroborating with the data already presented in the literature 14 .…”

“…This experiment showed a similar index to the short-term dehiscence that can be observed in the literature, demonstrating that the adhesive is less efficient in areas of tension than the conventional suture, limiting its isolated use to areas of low tension. Ilgenfritz et al 14 ,. when performing a similar experiment with the same N-2-butyl cyanoacrylate and Octyl-cyanoacrylate adhesive, found a total of 33.3% dehiscence with Octyl-cyanoacrylate alone and 41.6% with N-2-butylcyanoacrylate 14, and in this experiment 12.5% dehiscence was observed in group I and 25% in group II, groups where the adhesives were used in isolation.…”

“…The rupture strength corresponds to the most significant force value required during the sample traction, i.e., the highest strength value of the sample to achieve rupture. The traction occurred at a speed of 50mm/min, and the traction force was continuously measured automatically 14 .…”

Análise histológica comparativa do reparo tecidual após síntese com fio de nylon e adesivo de Etil-2-cianoacrilato em feridas cutâneas de ratos

“…Em regiões teciduais, sobretudo as mais sensíveis, a busca pela estética final e agilidade do processo de reparo são fundamentais, e o uso de materiais que proporcionem o mínimo de dano e reação inflamatória local na aproximação dos bordos da ferida deve ser avaliado. 8,9 A cicatrização é um estado dinâmico que compreende diversos processos, tais como inflamação, proliferação celular e síntese dos elementos constituintes da matriz extracelular, como o colágeno, as fibras elásticas e reticulares. A síntese do colágeno é processada assim que a lesão intersticial começa e este processo acontece até as fases cicatriciais finais, enquanto a remodelação do tecido ocorre.…”

Histological analysis of biocompatibility of different surgical adhesives in subcutaneous tissue

“…However, there is a lack of in vivo studies about the influence of surgical adhesives on the inflammatory and cure processes (Balakrishnan et al, ; Bartenstein et al, ; Ilgenfritz et al, ). Therefore, the focus of this double blind randomized study was to test the hypothesis that there would be no difference between the biocompatibility of CA‐based adhesives with different alkyl, n‐butyl, n‐hexyl and ethyl chain lengths in subcutaneous tissue in different time intervals.…”