The apoptotic and anti-proliferative activity of Origanum majorana extracts on human leukemic cell line

Abdel-Massih, Roula M.; Fares, Rida; Bazzi, Samer; El-Chami, Nisrine; Baydoun, Elias

doi:10.1016/j.leukres.2009.09.018

Cited by 59 publications

(39 citation statements)

References 27 publications

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“…Natural products have promising results as potential treatment for cancer in general and ALL in particular (Jadhav et al, 2007;Tai et al, 2010;Abdel-Massih et al, 2010;Davicino et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Pleurotus ostreatus and Ruscus aculeatus Extracts Cause Non-Apoptotic Jurkat Cell Death

Bassil¹,

Abdel-Massih²,

El-Chami³

et al. 2012

JPS

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We tested the effect of ethanol extracts from Ruscus aculeatus (Asparagaceae) and Pleurotus ostreatus (Agaricales) on the survival and proliferation of the Jurkat cell line, a model of human acute T-cell lymphocytic leukemia. R. aculeatus extracts were toxic to both Jurkat cells and human primary lymphocytes. P. ostreatus extracts were significantly more toxic to Jurkat cells than primary lymphocytes, as seen by LDH release and MTT assays. Annexin-V detection as well as Bax, Bcl-2, Smac/Diablo, and Caspase-8 expression were largely unchanged by P. ostreatus extracts. Cell cycle analysis and CDK-4 expression revealed stop of Jurkat cell proliferation. Interestingly, both extracts cause a strong, dose-dependent decrease in p53 levels in Jurkat cells, consistent with toxicity and cell death occurring via an unknown, non-apoptotic mechanism.

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Section: Introductionmentioning

confidence: 99%

Pleurotus ostreatus and Ruscus aculeatus Extracts Cause Non-Apoptotic Jurkat Cell Death

Bassil¹,

Abdel-Massih²,

El-Chami³

et al. 2012

JPS

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“…The terpenoid phenols tested were able to inhibit biofilms of several strains of Candida, including C. albicans, C. glabrata, and C. parapsilosis. In addition to their antimycotic, antibacterial, insecticidal, and bioherbicidal properties, essential oils are also well known for their antioxidant characteristics and are used to inhibit lipid peroxidation in preventing food spoilage or as chemoprotective agents in the treatment of various diseases, including cancer (1,26).…”

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confidence: 99%

Mechanism of Antifungal Activity of Terpenoid Phenols Resembles Calcium Stress and Inhibition of the TOR Pathway

Rao

Zhang

Muend

et al. 2010

Antimicrob Agents Chemother

256

141

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Terpenoid phenols, including carvacrol, are components of oregano and other plant essential oils that exhibit potent antifungal activity against a wide range of pathogens, including Candida albicans, Staphylococcus aureus, and Pseudomonas aeruginosa. To gain a mechanistic view of the cellular response to terpenoid phenols, we used Saccharomyces cerevisiae as a model organism and monitored temporal changes in metabolic activity, cytosolic and vacuolar pH, and Ca 2؉ transients. Using a panel of related compounds, we observed dosedependent Ca 2؉ bursts that correlated with antifungal efficacy. Changes in pH were long lasting and followed the Ca 2؉ transients. A vma mutant lacking functional vacuolar H ؉ -ATPase (V-ATPase) and defective in ion homeostasis was hypersensitive to carvacrol toxicity, consistent with a role for ionic disruptions in mediating cell death. Genomic profiling within 15 min of exposure revealed a robust transcriptional response to carvacrol, closely resembling that of calcium stress. Genes involved in alternate metabolic and energy pathways, stress response, autophagy, and drug efflux were prominently upregulated, whereas repressed genes mediated ribosome biogenesis and RNA metabolism. These responses were strongly reminiscent of the effects of rapamycin, the inhibitor of the TOR pathway of nutrient sensing. The results point to the activation of specific signaling pathways downstream of cellular interaction with carvacrol rather than a nonspecific lesion of membranes, as has been previously proposed.While the medicinal properties of herbs have been recognized since ancient times, there has been a resurgence of interest in the antimicrobial properties of botanical extracts. Essential oils have been amply documented to kill a wide range of pathogenic fungi and bacteria, such as Candida albicans, Staphylococcus aureus, and Pseudomonas aeruginosa, including their drug-resistant variants (6,10,21,22). Of the herbal extracts tested, essential oils derived from the genus Oreganum were among the most effective, with an in vitro MIC of 500 ppm against C. albicans (27). Major components of oregano extract, which include the terpenoid phenols carvacrol, thymol, and eugenol, have potent antifungal activity of their own (4, 23, 24). Terpenoid phenols have been shown to be efficacious not only on planktonic cells but also on biofilms of Candida albicans that are resistant to many antifungal drugs. Carvacrol demonstrated the strongest antifungal activity against Candida albicans biofilms, with a MIC of Ͻ0.03% (9). Furthermore, carvacrol was shown to be effective regardless of the maturity of the biofilm. The terpenoid phenols tested were able to inhibit biofilms of several strains of Candida, including C. albicans, C. glabrata, and C. parapsilosis. In addition to their antimycotic, antibacterial, insecticidal, and bioherbicidal properties, essential oils are also well known for their antioxidant characteristics and are used to inhibit lipid peroxidation in preventing food spoilage or as chemoprotective agen...

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“…O. majorana L. essential oil was found to be more cytotoxic than Origanum vulgare L. essential oil against different cancer cell types, such as MCF-7, LNCaP and NIH-3T3 (Hussain et al 2011). Plant extracts from O. majorana showed cytotoxicity and anti-proliferative effect on Jurkat cells (Abdel-Massih et al 2010). In another study, thymol, carvacrol, eugenol, eucalyptol, terpinen-4-ol and camphor (i.e.…”

Section: Resultsmentioning

confidence: 93%

A comparative study of cytotoxic, membrane and DNA damaging effects of Origanum majorana’s essential oil and its oxygenated monoterpene component linalool on parental and epirubicin-resistant H1299 cells

Erdoğan

Özkan

2013

Biologia

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In this study, cytotoxic, membrane and DNA damaging effects of the essential oil from Origanum majorana and its oxygenated monoterpene component linalool were tested on parental and epirubicin-resistant (drug-resistant) human lung cancer cell lines (H1299). Essential oil's and linalool's cytotoxicities were examined and parental cells were found more sensitive to the essential oil's and linalool's cytotoxicities than drug-resistant cells. O. majorana essential oil had more effective membrane damaging effect than linalool on parental cells, while in drug-resistant H1299 cells, linalool had more effective membrane damaging effect than the essential oil. O. majorana essential oil possessed more effective DNA damaging effect than linalool on both parental and drug-resistant cells. The conclusions from this study suggest that O. majorana essential oil and linalool exhibit cytotoxic, membrane and DNA damaging effects. They thus need further investigation as potential therapeutic agents for human lung cancer.

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The apoptotic and anti-proliferative activity of Origanum majorana extracts on human leukemic cell line

Cited by 59 publications

References 27 publications

Pleurotus ostreatus and Ruscus aculeatus Extracts Cause Non-Apoptotic Jurkat Cell Death

Pleurotus ostreatus and Ruscus aculeatus Extracts Cause Non-Apoptotic Jurkat Cell Death

Mechanism of Antifungal Activity of Terpenoid Phenols Resembles Calcium Stress and Inhibition of the TOR Pathway

A comparative study of cytotoxic, membrane and DNA damaging effects of Origanum majorana’s essential oil and its oxygenated monoterpene component linalool on parental and epirubicin-resistant H1299 cells

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