The Apoptotic Effect of 1’S-1’-Acetoxychavicol Acetate from Alpinia Conchigera on Human Cancer Cells

Abstract

1’-(S)-1’-Acetoxychavicol acetate (ACA) isolated from the Malaysian ethno-medicinal plant Alpinia conchigera Griff. was investigated for its potential as an anticancer drug. In this communication, we describe the cytotoxic and apoptotic properties of ACA on five human tumour cell lines. Data from MTT cell viability assays indicated that ACA induced both time- and dose-dependent cytotoxicity on all tumour cell lines tested and had no adverse cytotoxic effects on normal cells. Total mortality of the entire tumour cell population was achieved within 30 hrs when treated with ACA at 40.0 µM concentration. Flow cytometric analysis for annexin-V and PI dual staining demonstrated that cell death occurred via apoptosis, followed by secondary necrosis. The apoptotic effects of ACA were confirmed via the DNA fragmentation assay, in which consistent laddering of genomic DNA was observed for all tumour cell lines after a 24 hrs post-treatment period at the IC50 concentration of ACA. A cell cycle analysis using PI staining also demonstrated that ACA induced cell cycle arrest at the G0/G1 phase, corresponding to oral tumour cell lines. In conclusion, ACA exhibits enormous potential for future development as a chemotherapeutic drug against various malignancies.

Suppression of microRNA-629 enhances sensitivity of cervical cancer cells to 1′S-1′-acetoxychavicol acetate via regulating RSU1

“…The 1′S-1′-acetoxychavicol acetate (ACA) is a natural compound isolated from the wild ginger, Alpinia conchigera . We have previously reported that it is able to induce cytotoxicity when used as stand-alone and potentiates the effects of cisplatin when used in combination on different cancer cells including cervical cancer 6,7…”

1’-Acetoxychavicol acetate inhibits growth of human oral carcinoma xenograft in mice and potentiates cisplatin effect via proinflammatory microenvironment alterations

“…The use of 1’S-1’-acetoxychavicol acetate (ACA), which is a phenylpropanoid naturally found within various Zingiberaceae family members, has been traditionally associated with a number of various medicinal properties including anti-ulceration [15], anti-allergic [16], anti-inflammatory and anti-cancer activities [17,18]. Previous studies have shown ACA to be associated with the production of intracellular reactive oxygen species (ROS) [19], inhibition of xanthane oxidase (XO) activity [20], inhibition of nitric oxide synthase (NOS) expression [21], inhibition of polyamine synthesis [22], induction of apoptosis via mitochondrial/Fas-mediated dual mechanism [19] and as a potential NF-κB inhibitor [23-25].…”

Profil Toksikologi Ekstrak Heksana Alpinia conchigera (Lengkuas Kecil) Secara In vitro

“…Berdasarkan data yang diperoleh, didapati bahawa mod kematian secara apoptosis merupakan mod kematian utama bagi sel hepar Chang setelah dirawat dengan esktrak heksana A. conchigera (Rajah 2). Hasil kajian ini adalah selari dengan kajian yang dilakukan oleh Khalijah et al (2010) iaitu kematian sel yang disebabkan oleh 1'-S-1'-Acetoxychavicol Acetate daripada ekstrak A. conchigera adalah secara apoptosis. Selain itu, kajian ini turut selari dengan kajian oleh In et al (2011), metabolit tunggal iaitu AEA yang diasingkan daripada ekstrak rizom A. conchigera telah menyebabkan kematian sel adenokarsinoma payudara (MCF-7) secara apoptosis.…”