The appearance of polygalacturonase mRNA in tomatoes: one of a series of changes in gene expression during development and ripening

Grierson, Donald; Slater, A.; Speirs, Jim; Tucker, Gregory A.

doi:10.1007/bf00393517

Cited by 109 publications

(99 citation statements)

References 16 publications

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“…Total RNA: Total RNA was extracted from peanut seeds harvested from the control or mineral salt-treated boxes using the acidic phenol/chloroform (pH 4.5) method [49].…”

Section: Methodsmentioning

confidence: 99%

Horticultural Production of Ultra High Resveratrol Peanut

Osuji¹,

Johnson²,

Duffus³

et al. 2017

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Background: Resveratrol naturally occurring antioxidant in peanut (Legume:Arachis hypogaea) has phytochemical human health dietary effects associated with reduced inflammatory cancer risks. Its levels in peanut are ultra-low and variable (0 to 26 µg•g −1 ), which has made it difficult to market as a consistent high resveratrol produce. Objective: Understanding the regulation of resveratrol accumulation in peanut might lead to development of new techniques for optimizing and stabilizing its yield. Method: Peanuts were cultivated in horticultural field plots and treated with solutions of mineral salts (sulfate, potassium, phosphate, ammonium ion) that were optimized in stoichiometric (reactive) ratios. Peanut seed's RNAs were subjected to Northern blot analysis for profiling the RNAs synthesized by glutamate dehydrogenase (GDH), and mRNAs encoding resveratrol synthase. The seed's extracts were analyzed by GC-MS for determination of the resveratrol and fatty acid compositions. Result: Stoichiometric mixes of mineral ions induced the peanut GDH to synthesize some RNA that silenced the mRNAs encoding resveratrol synthase, phosphoglucomutase, isocitrate lyase, malate synthase, enolase, phosphoenolpyruvate carboxylase, malate dehydrogenase, and phosphoglycerate mutase in the control, KN-, and NPKS-treated but not in the NPPK-treated peanut. These resulted to decreased resveratrol content (6.0 µg•g −1

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“…Total RNA: Total RNA was extracted from peanut seeds harvested from the control or mineral salt-treated boxes using the acidic phenol/chloroform (pH 4.5) method [49].…”

Section: Methodsmentioning

confidence: 99%

Horticultural Production of Ultra High Resveratrol Peanut

Osuji¹,

Johnson²,

Duffus³

et al. 2017

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show abstract

“…These coordinated physiological and biochemical changes are due to differential gene expression, as has been shown by analyses of total proteins and in vitro translation products synthesized from poly(A)+ RNAs isolated from ripening tomato (Lycopersicon esculentum) fruits (Grierson et al, 1985;Biggs et al, 1986). Differential screening and subtractive hybridization of cDNA libraries made from poly(A)+ RNAs of ripening tomato fruits have led to the isolation and characterization of cDNAs for many genes in which the expression changes during fruit development and ripening (Gray et al, 1992).…”

mentioning

confidence: 99%

Molecular Cloning of a Ripening-Specific Lipoxygenase and Its Expression during Wild-Type and Mutant Tomato Fruit Development

Kausch

Handa

1997

Plant Physiology

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A 94-kD protein that accumulates predominately in tomato (Lycopersicon esculenfum) fruit during ripening was purified, and antibodies specific for the purified protein were used to isolate cDNA clones from a red-ripe fruit cDNA library. A sequence analysis of these cDNAs and cross-reactivity of the 94-kD-specific antibodies to the soybean lipoxygenase (LOX) L-1, L-2, and L-3 proteins and soybean LOX L-l-specific antibodies to the 94-kD protein identified it as a member of the LOX gene family. Maximum levels of the 94-kD LOX mRNA and protein are present in breaker to ripe and red-ripe stages, respectively. Expression of 94-kD LOX in different tissues from mature green and red-ripe tomato fruits was found to be greatest in the radial walls of ripe fruit, but immunocytolocalization using tissue printing suggests that the highest accumulation of its protein occurs in locular jelly. None of 94-kD LOX is expressed in nonripening mutant fruits of any age. Never-ripe mutant fruit accumulate the 94-kD LOX mRNA to levels similar to those obtained in wild-type fruit, but fail to accumulate the 94-kD LOX protein. Collectively, the results show that expression of 94-kD LOX is regulated by the ripening process, and ethylene may play a role in its protein accumulation.

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“…Tomato plants (Lycopersicon esculentum Mill, variety Ailsa Craig) were grown as described previously (13) (11).…”

mentioning

confidence: 99%

Identification of a tomato gene for the ethylene-forming enzyme by expression in yeast.

Hamilton

Bouzayen

Grierson

1991

Proc. Natl. Acad. Sci. U.S.A.

265

144

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The appearance of polygalacturonase mRNA in tomatoes: one of a series of changes in gene expression during development and ripening

Cited by 109 publications

References 16 publications

Horticultural Production of Ultra High Resveratrol Peanut

Horticultural Production of Ultra High Resveratrol Peanut

Molecular Cloning of a Ripening-Specific Lipoxygenase and Its Expression during Wild-Type and Mutant Tomato Fruit Development

Identification of a tomato gene for the ethylene-forming enzyme by expression in yeast.

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