The apple 14-3-3 gene MdGRF6 negatively regulates salt tolerance

Zhu, Yuqing; Kuang, Wei; Leng, Jun; Wang, Xue; Qiu, Lugui; Kong, Xin Ying; Wang, Yongzhang; Zhao, Qiang

doi:10.3389/fpls.2023.1161539

Cited by 6 publications

(1 citation statement)

References 78 publications

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“…The regulation of the localization and stability of 14-3-3s is another layer of the salt resistance mechanism in plants [ 15 ]. In addition, the negative and positive regulation of MdGRF6 and TaGRF6-A during salt-induced stress, respectively [ 16 , 17 ], indicate the diverse functions of 14-3-3s under salt stress. Additionally, the heterologous expression of At14-3-3λ in cotton enhanced the tolerance to drought-induced stress [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

The 14-3-3 Protein BdGF14a Increases the Transcriptional Regulation Activity of BdbZIP62 to Confer Drought and Salt Resistance in Tobacco

Zhang,

He,

Zhao

et al. 2024

Plants

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BdGF14a, a 14-3-3 gene from Brachypodium distachyon, induced by salt, H2O2, and abscisic acid (ABA), improved tolerance to drought and salt in tobacco, with a higher survival rate and longer roots under these stresses. Additionally, physiological index analyses showed that the heterologous expression of BdGF14a induced higher expression levels of antioxidant enzymes and their activities, leading to lighter DAB and NBT staining, denoting decreased H2O2 content. Additionally, the lower MDA content and ion leakage indicated enhanced cell membrane stability. Moreover, exogenous ABA resulted in shorter roots and a lower stomatal aperture in BdGF14a transgenic plants. BdGF14a interacted with NtABF2 and regulated the expression of stress-related genes. However, adding an ABA biosynthesis inhibitor suppressed most of these changes. Furthermore, similar salt and drought resistance phenotypes and physiological indicators were characterized in tobacco plants expressing BdbZIP62, an ABRE/ABF that interacts with BdGF14a. And Y1H and LUC assays showed that BdGF14a could enhance the transcription regulation activity of NtABF2 and BdbZIP62, targeting NtNECD1 by binding to the ABRE cis-element. Thus, BdGF14a confers resistance to drought and salinity through interaction with BdbZIP62 and enhances its transcriptional regulation activity via an ABA-mediated signaling pathway. Therefore, this work offers novel target genes for breeding salt- and drought-tolerant plants.

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Section: Introductionmentioning

confidence: 99%

The 14-3-3 Protein BdGF14a Increases the Transcriptional Regulation Activity of BdbZIP62 to Confer Drought and Salt Resistance in Tobacco

Zhang,

He,

Zhao

et al. 2024

Plants

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Molecular evolution and interaction of 14-3-3 proteins with H+-ATPases in plant abiotic stresses

Jiang,

He,

Babla

et al. 2023

Journal of Experimental Botany

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Environmental stresses severely affect plant growth and crop productivity. Regulated by 14-3-3 proteins (14-3-3s), H+-ATPases (AHA) are important proton pumps that can induce diverse secondary transport via channels and co-transporters for the abiotic stress response of plants. Many studies demonstrated the roles of 14-3-3s and AHAs in coordinating the processes of plant growth, phytohormone signaling, and stress responses. However, the molecular evolution of 14-3-3s and AHAs has not been summarized in parallel with evolutionary insights across multiple plant species. Here, we comprehensively review the roles of 14-3-3s and AHAs in cell signaling to enhance plant responses to diverse environmental stresses. We analyzed the molecular evolution of key proteins and functional domains that are associated with 14-3-3s and AHAs in plant growth and hormone signaling. The results revealed evolution, duplication, contraction, and expansion of 14-3-3s and AHAs in green plants. We also discussed the stress-specific expression of those 14-3-3s and AHAs in a eudicot (Arabidopsis thaliana), a monocot (Hordeum vulgare) and a moss (Physcomitrium patens) under abiotic stresses. We propose that 14-3-3s and H+-ATPases respond to abiotic stresses through many important targets and signaling components of phytohormones, which could be promising to improve plant tolerance to single or multiple environmental stresses.

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The 14-3-3 protein GRF8 modulates salt stress tolerance in apple via the WRKY18-SOS pathway

Fan,

Zhu,

Kuang

et al. 2023

Plant Physiology

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Salinity is a severe abiotic stress that limits plant survival, growth, and development. 14-3-3 proteins are phosphopeptide-binding proteins that are involved in numerous signaling pathways, such as metabolism, development, and stress responses. However, their roles in salt tolerance are unclear in woody plants. Here, we characterized an apple (Malus domestica) 14-3-3 gene, GENERAL REGULATORY FACTOR 8 (MdGRF8), the product of which promotes salinity tolerance. MdGRF8 overexpression improved salt tolerance in apple plants, whereas MdGRF8-RNA interference (RNAi) weakened it. Yeast 2-hybrid, bimolecular fluorescence complementation, pull-down, and coimmunoprecipitation assays revealed that MdGRF8 interacts with the transcription factor MdWRKY18. As with MdGRF8, overexpressing MdWRKY18 enhanced salt tolerance in apple plants, whereas silencing MdWRKY18 had the opposite effect. We also determined that MdWRKY18 binds to the promoters of the salt-related genes SALT OVERLY SENSITIVE 2 (MdSOS2) and MdSOS3. Moreover, we showed that the 14-3-3 protein MdGRF8 binds to the phosphorylated form of MdWRKY18, enhancing its stability and transcriptional activation activity. Our findings reveal a regulatory mechanism by the MdGRF8–MdWRKY18 module for promoting the salinity stress response in apple.

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The apple 14-3-3 gene MdGRF6 negatively regulates salt tolerance

Cited by 6 publications

References 78 publications

The 14-3-3 Protein BdGF14a Increases the Transcriptional Regulation Activity of BdbZIP62 to Confer Drought and Salt Resistance in Tobacco

The 14-3-3 Protein BdGF14a Increases the Transcriptional Regulation Activity of BdbZIP62 to Confer Drought and Salt Resistance in Tobacco

Molecular evolution and interaction of 14-3-3 proteins with H+-ATPases in plant abiotic stresses

The 14-3-3 protein GRF8 modulates salt stress tolerance in apple via the WRKY18-SOS pathway

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