The application of a lateral flow immunographic assay to rapidly test for dexamethasone in commercial facial masks

Abstract

Dexamethasone (DE) is a synthetic glucocorticoid that is frequently added to cosmetic products for its good short-term effects, especially in facial masks, but long-term use is hazardous to the health. The abuse of DE in whitening and acne cosmetic products is currently a serious problem in China. It is necessary to establish a rapid method of detecting illegal DE addition in cosmetics. In the present study, a monoclonal antibody (mAb) against DE, 2D5-3D12, was developed that displayed cross-reactivities of 124.5%, 38.8%, 6.7%, 0.9%, 1.1%, 1.82%, and 2.39% with prednisolone, betamethasone, prednisone, beclomethasone, hydrocortisone, triamcinolone, and flumetasone, respectively. A colloidal gold-based lateral flow immunographic assay based on mAb 2D5-3D12 was established and used to determine the DE contents of commercial facial masks. The indicator range of the immunographic assay for DE was 100–200 ng/mL, and the results were consistent with those afforded by LC-MS. This novel method provides the advantages of simple sample treatment, a user-friendly procedure, and rapid detection. Graphical abstract Electronic supplementary material The online version of this article (10.1007/s00216-019-01948-2) contains supplementary material, which is available to authorized users.

A novel multi-layer perceptron model for assessing the diagnostic value of non-invasive imaging instruments for rosacea

“…We used the VISIA system to evaluate the deep blood vessels of rosacea by assessing the confluent and diffuse background erythema, and found that the severity of the background erythema and cheek involvement were supportive evidence for rosacea diagnosis. Telangiectasis is another important presentation of rosacea under VISIA, which can often be observed in control subjects with sun-damaged facial skin (such as photoaging) or glucocorticosteroid-induced dermatitis ( Helfrich et al, 2015 ; Lahiri & Coondoo, 2016 ; Wang et al, 2019 ). However, the telangiectasis displayed by VISIA for rosacea were preferably located in the upper eyelid ( Fig.…”

Effects of skin care habits on the development of rosacea: A multi-center retrospective case-control survey in Chinese population

“…According to the National Food and Drug Administration website of China, from 2016 to 2018, glucocorticoids were illegally added to many batches of cosmetic products. Notably, the abuse of glucocorticoid was particularly prevalent in facial masks and beauty salon products for short-term commercial effects [37,38]. It has well accepted that dilation of blood vessels, red spots, and thinning of the facial skin like the feature of ETR would take place in the process of long-term use of glucocorticoid [38].…”

“…Notably, the abuse of glucocorticoid was particularly prevalent in facial masks and beauty salon products for short-term commercial effects [37,38]. It has well accepted that dilation of blood vessels, red spots, and thinning of the facial skin like the feature of ETR would take place in the process of long-term use of glucocorticoid [38]. Therefore, if the source and the contents of skin care products are unreliable, the more frequent and more regular skin care people do, the more likely they will suffer skin barrier damage and even attack steroid-induced rosacea.…”

Tailor-Made Immunochromatographic Test for the Detection of Multiple 17α-Methylated Anabolics in Dietary Supplements

“…Due to the ease of use and commercial attractiveness of immunochromatographic assays [ 11 ], research and development in this area has expanded considerably. A wide range of analytes including viruses (e.g., hepatitis B [ 12 ], HIV [ 13 ], SARS-CoV-2 [ 14 ]), bacteria (e.g., Helicobacter pylori [ 15 ], Staphylococcus aureus [ 16 ], Salmonella [ 17 ]), toxins [ 18 , 19 , 20 ], pesticides [ 21 ], cancer markers [ 22 , 23 ], antibiotics [ 24 , 25 ], drugs [ 26 , 27 ], terpenes [ 28 ] and steroids [ 29 , 30 , 31 , 32 , 33 ] have been used to construct useful immunochromatographic assays.…”