The application of a pCR technique for the detection of immunoglobulin heavy chain gene rearrangements in fresh or paraffin-embedded skin tissue

A comparative study of the quality of DNA obtained from fresh frozen and formalin-fixed decalcified paraffin-embedded bone marrow trephine biopsy specimens using two different methods

“…Although this appears obvious, previous reports on skin biopsy specimens and on aspirate clots have shown that there is no statistically significant difference in amplifiable DNA extracted from fresh frozen and paraffin-embedded tissue 14 15. This suggests that it is the process of decalcification that most likely degrades the genomic DNA.…”

The many faces of lymphocytoma cutis

“…Characteristically, the dermal cellular infiltrate show a mixture of λ and κ light chain positive B cells, which contrasts to the predominance of κ or λ light chains in CBCL. This immunohistochemical technique became the gold standard to differentiate benign from malignant cutaneous B‐cell disorders 10,11 …”

Conjunctival benign reactive lymphoid hyperplasia associated with myopic scleral thinning

“…Polymerase chain reaction (PCR) and immunoperoxidase studies on formalin‐fixed tissue showed it to be of a polyclonal nature, excluding lymphoma. PCR performed on formalin‐fixed tissues has previously been shown to achieve comparable results to fresh tissue samples 7 . The final diagnosis was conjunctival benign reactive lymphoid hyperplasia.…”