The Application of Bioremediationofoilsluge in Al-Dura Refineryinsomeplant

Abstract

Our research was accomplished after fifth months of continuous working from the 10 th of February to the 10 th of July.16 th samples were collected from different places at Al-Dura refinery which included tanks, soils, refinery operation stages, waste accumulation area. The results could be summarized as follows: 50% of the collected samples had solid and semisolid in nature included soil and oily sludge. While the other 50% of the collected samples had a liquid in nature. The pH of the collected samples ranged from 4 to 14. The percentage of heavy metals concentration in the sludge waste samples were zinc 0.0017, ferrous 0.04, magnesium 0.0038, tin 0.0004, chromium 0.0003, cupper 0.0009, nickel 0.0006 and lead 0.0001. While the heavy metals concentration in the sludge tanks were 0.002, 0.042, 0.0064, 0.0002, 0.0016, 0.0007 and 0.0001 respectively. All the collected samples showed heavy growth after culturing on different agar media with pH=7 incubated for 24 hours at 37 C°. Different microbes were isolated included bacteria (both Gram -ve and +ve), fungi yeast and algae which their isolation percentages were 100%, 20%, 5%, 1% respectively. Depending on the microscopic examination, growth on different agar media and results of the biochemical tests, many isolated species were identified according to Bergey's manual for bacterial isolates. The Gram-ve isolated bacterial species were Pseudomonas aeruginosa, Pseudomonas fluorescence, E. coli, Providencia, Xanthomonas and Proteus while Gram+ve bacterial species were Micrococcus, Methylococcus, Bacillus and Peptococcus. The isolated fungi were Penicillium, Aspergillus, Mucor and Rhizopus. The isolated yeast was Saccharomyces.The isolated alga was Spirogera. Rapid -One system was applied to the bacterial strains selected for bioremediation process.