The application of chromatography to amino acids and peptides

Chromatographic Systems

“…Paper chromatography has facilitated the isolation of peptides as well as of amino acids (203) as in animal organs (134), enzyme syntheses (49,162) analogous to solvolytic reactions (17,162), and chemical preparations (164). It has aided the isolation of the hydrolysis products of proteins (252,312) as ferritin (136), pituitary protein (37), gramicidin (175), lipoxidase (185), egg albumin and others (308,312), horse-radish peroxidase (274), tobacco mosaic virus protein (367), insulin (133), the mitochondria of tissues (258), and the proteins of soil (48).…”

“…In paper chromatography there is a list of these chemical reagents nearly as long as the kinds of substances investigated, and these can easily be found by reference to the original papers. Reagents most widely used are ninhydrin, ammoniacal silver solutions (203), permanganate, iodine (279), and various colored derivatives. PROGNOSTICATIONS Owing to its speed, selectivity, sensitivity, adaptability, and reliability, chromatography is certain to reveal numerous new phenomena that will challenge the analytical skill and the interpretative capacity of scientists.…”

Biochemical Analysis

“…Ts review the field in detail as it affects biochemical analy-sis is next to impossible. Numerous general review's of technique and application are already' available (24,25,102,131,186,263). It is sufficient here to note some of the more important and unusual developments that may' indicate the trends and the direction of increased future application.…”

An Ultramicro Method for Quantitative Determination of Amino Acids

“…T o establish the positions of a~n i n o acids, Fowden relied on their fluorescence on filter paper after heating. This is not a reliable method since amino acids do not fluoresce on all papers nor do they all fluoresce in low co~lcentrations (21). Boissonnas (7) devised an ingenious "hedgehog" for spotting the paper with ninhydrin as a means of locating amino acids.…”