The application of dried amniotic membrane scaffold with adipose derived-mesenchymal stem cell seeding as graft in urethral reconstruction (experiment on rabbit)

Abstract

Background: Urethral reconstruction has been a problem for a long time and is a major challenge for surgeons. Surgical management of urethral defects is still controversial because there is no synthetic material that is considered ideal as a substitute for the urethra. With the development of adipose-derived mesenchymal stem cells, this research aims to observe the difference between the use of dried amniotic membrane as a scaffold with and without mesenchymal stem cells seeding as graft in urethral reconstruction. Methods: This research was a pure experimental research with a post-test only control group design using 24 male New Zealand white rabbits. The samples were divided into 3 groups: one control group, one scaffold (dried amniotic membrane) without stem cell seeding group, and one stem cell-seeded scaffold group. The rabbits were evaluated at 28 days after treatment and the results were analyzed using Chi-Square test. Results: The number of urethras that were healed were higher in stem cell-seeded scaffold compared to scaffold without stem cell seeding group (p < 0.05). Conclusion: Considering the promising result, application of dried amniotic membrane as a scaffold with adipose-derived mesenchymal stem cell seeding could be an option for urethral reconstruction. Highlights:

Decellularized Placental Sponge Seeded with Human Mesenchymal Stem Cells Improves Deep Skin Wound Healing in the Animal Model

“…Several studies have reported on the cytobiocompatibility and cell adhesion properties of ECM-based scaffolds. , In a study conducted by Rameshbabu et al, the adhesion of DPS for human amniotic mesenchymal stem cells (HAMSCs) was reported. They fabricated human placental-derived extracellular matrix sponges (PEMS) for bone regeneration application.…”

Application of amniotic membranes in reconstructive surgery of internal organs—A systematic review and meta‐analysis

“…The PRISMA flowchart is shown in Figure 2. Sixty references containing 26 human studies (Ambrus, 1967; Barski et al., 2015; Catalano & Conticello, 1968; De Weerd et al., 2013, 2020; Eichberg et al., 2018, 2019; Frigerio et al., 2019; Ghahramani et al., 2017; Kakabadze et al., 2016; Kumar et al., 2015; Marton et al., 2021; Pignatti et al., 2020; Price & Price, 2016; Razzaghi et al., 2020; Rohleder et al., 2013; Schoellhammer et al., 2018; Schrimpf, 1954; Singh et al., 2011; Tahami et al., 2020; Tomita et al., 2012; Ts'ao, 1960; Tumanov et al., 2006; Turchan et al., 2018; Walker et al., 2018; Zmijewski & Pietraszek, 2005) and 36 animal studies (Barski et al., 2017; Brown et al., 2014; Demir et al., 2021; Ercin et al., 2019; Erdener et al., 1992; Ferhatoglu et al., 2019; Fishman et al., 1987; Francisco et al., 2016; Ghahramani et al., 2014; Güneş et al., 2016, 2017; Hariastawa et al., 2020; Iijima et al., 2007; Ismail, Marcos, et al., 2009; Ismail, Ramsis, et al., 2009; Kesting et al., 2008, 2010; Li et al., 2019; Liu et al., 2021; Mohajeri et al., 2014; Moslemi et al., 2016; Orman et al., 2020; Rohleder et al., 2013; Roshanravan et al., 2014; Schimidt et al., 2010; Scott et al., 2021; Scudamore et al., 1988; Senthil‐Kumar et al., 2016; Tomita et al.,…”

“…Price, 2016;Razzaghi et al, 2020;Rohleder et al, 2013;Schoellhammer et al, 2018;Schrimpf, 1954;Singh et al, 2011;Tahami et al, 2020;Tomita et al, 2012;Ts'ao, 1960;Tumanov et al, 2006;Turchan et al, 2018;Walker et al, 2018;Zmijewski & Pietraszek, 2005) and 36 animal studies(Barski et al, 2017;Brown et al, 2014;Demir et al, 2021;Ercin et al, 2019;Erdener et al, 1992;Ferhatoglu et al, 2019;Fishman et al, 1987; Francisco et al, 2016;Ghahramani et al, 2014;Güneş et al, 2016Güneş et al, , 2017Hariastawa et al, 2020;Iijima et al, 2007;Ismail, Marcos, et al, 2009;Ismail, Ramsis, et al, 2009;Kesting et al, 2008Kesting et al, , 2010Li et al, 2019;Liu et al, 2021;Mohajeri et al, 2014;Moslemi et al, 2016;Orman et al, 2020;Rohleder et al, 2013;Roshanravan et al, 2014;Schimidt et al, 2010;Scott et al, 2021;Scudamore et al, 1988;Senthil-Kumar et al, 2016;…”

Future of Bovine Amniotic Membrane: Bovine Membrane Application on Wound Healing, Surgery and Prospect of Use for Urethral Reconstruction

“…Neovascularization and best epithelial transformation was seen in combined amniotic membrane and adipose-derived mesenchymal stem cell seeding group. The promising result showed that stem cell could be used as adjunct treatment for amniotic membrane application [84].…”