The application of electron spectroscopic imaging for quantification of the area fractions of calcium‐containing precipitates in nervous tissue

Körtje, Karl‐Heinz; Körtje, Doris

doi:10.1111/j.1365-2818.1992.tb01533.x

Cited by 20 publications

(15 citation statements)

References 27 publications

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“…This final image representing the distribution of calcium in the tissue was visually compared to micrographs obtained by structural imaging of the same area, displaying the arrangement of EDDs. To match the resolution of the structural image to the digital analytical images (512 Â 512 pixel), fine structural images were obtained in ESI mode of the microscope, as well, tuned to the carbon absorption edge (250 eV) (Ko¨rtje and Ko¨rtje, 1992). Such images, resembling ''dark-field'' images, were then inverted for easier comparison.…”

Section: Electron Spectroscopic Imaging Control Of the Electron Histomentioning

confidence: 99%

Intra-axonal calcium changes after axotomy in wild-type and slow Wallerian degeneration axons

et al. 2012

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Section: Electron Spectroscopic Imaging Control Of the Electron Histomentioning

confidence: 99%

Intra-axonal calcium changes after axotomy in wild-type and slow Wallerian degeneration axons

et al. 2012

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“…To match the resolution of the structural image to the digital analytical images (512 Â 512 pixel), fine structural images were obtained in the spectroscopic mode of the analyzer as well, tuned to the carbon absorption edge (250 eV), which resulted in good-quality structural images of the sections (Körtje and Körtje, 1992). Such images, resembling ''darkfield'' images, were then inverted for easier comparison.…”

Section: Specificity Control Of the Histochemical Reaction For Calciummentioning

confidence: 99%

Hypoglossal motor neurons display a reduced calcium increase after axotomy in mice with upregulated parvalbumin

Paizs

Engelhardt

Katarova

et al. 2010

J of Comparative Neurology

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Motor neurons that exhibit differences in vulnerability to degeneration have been identified in motor neuron disease and in its animal models. The oculomotor and hypoglossal neurons are regarded as the prototypes of the resistant and susceptible cell types, respectively. Because an increase in the level of intracellular calcium has been proposed as a feature amplifying degenerative processes, we earlier studied the calcium increase in these motor neurons after axotomy in Balb/c mice and demonstrated a correlation between the susceptibility to degeneration and the intracellular calcium increase, with an inverse relation with the calcium buffering capacity, characterized by the parvalbumin or calbindin-D(28k) content. Because the differential susceptibility of the cells might also be attributed to their different cellular environments, in the present experiments, with the aim of verifying directly that a higher calcium buffering capacity is indeed responsible for the enhanced resistance, motor neurons were studied in their original milieu in mice with a genetically increased parvalbumin level. The changes in intracellular calcium level of the hypoglossal and oculomotor neurons after axotomy were studied electron microscopically at a 21-day interval after axotomy, during which time no significant calcium increase was detected in the hypoglossal motor neurons, the response being similar to that of the oculomotor neurons. The hypoglossal motor neurons of the parental mice, used as positive controls, exhibited a transient, significant elevation of calcium. These data provide more direct evidence of the protective role of parvalbumin against the degeneration mediated by a calcium increase in the acute injury of motor neurons.

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“…This final image representing the distribution of calcium in the tissue was visually compared with micrographs obtained by structural imaging of the same area, displaying the arrangement of EDDs. To match the resolution of the structural image to the digital analytical images (512 ϫ 512 pixel), fine structural images were obtained in spectroscopic mode of the analyzer, as well, tuned to the carbon absorption edge (250 eV), resulting in good quality structural images of the sections (Körtje and Körtje, 1992). Such images, resembling "darkfield" images, were then inverted for easier comparison.…”

Section: Specimen Preparation For Electron Microscopic Detection Of Tmentioning

confidence: 99%

Axotomy induces contrasting changes in calcium and calcium-binding proteins in oculomotor and hypoglossal nuclei of Balb/c mice

2006

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Motor neurons with different susceptibility to degeneration have been identified in amyotrophic lateral sclerosis (ALS). Increase of intracellular calcium has been proposed as a mediator, amplifying the damage through a positive feedback of the known pathological processes. Accordingly, the potential of motor neurons to limit calcium increases during injury might be proportional to their viability. A basic mechanism of reducing calcium amplitudes depends on the calcium-buffering capacity, determined by the calcium-binding protein content. In this study, oculomotor and hypoglossal neurons, prototypes of resistant and vulnerable motor neurons in ALS were examined in axotomy experiments. Total calcium-, parvalbumin-, and calbindin-D28k levels of motor neurons of adult mice were characterized by electron microscopic histochemistry and light microscopic immunostaining. In hypoglossal neurons, compared with oculomotor neurons, larger and more enduring increases of calcium were detected. The perikarya of hypoglossal neurons remained immunonegative for both parvalbumin and calbindin-D28k. Qualitatively, no major cell loss was noted after axotomy, but a decreased neuronal marker staining at days 1-14 suggested a reversible injury of hypoglossal neurons. Oculomotor neurons were not stained for calbindin-D28k but stained for parvalbumin in control conditions, staining which increased at postoperative days 7-14 before returning to baseline. Neuronal marker staining did not change in these cells during the observed period. The higher level of parvalbumin in resistant motor neurons and their ability to up-regulate parvalbumin after injury, paralleled by a smaller increase of intracellular calcium suggest that parvalbumin may have a protective effect in these cells.

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The application of electron spectroscopic imaging for quantification of the area fractions of calcium‐containing precipitates in nervous tissue

Cited by 20 publications

References 27 publications

Intra-axonal calcium changes after axotomy in wild-type and slow Wallerian degeneration axons

Intra-axonal calcium changes after axotomy in wild-type and slow Wallerian degeneration axons

Hypoglossal motor neurons display a reduced calcium increase after axotomy in mice with upregulated parvalbumin

Axotomy induces contrasting changes in calcium and calcium-binding proteins in oculomotor and hypoglossal nuclei of Balb/c mice

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