The application of ferrocene‐modified n‐type silicon in glucose biosensors

Abstract

This article describes the application of ferrocene-modified n-type silicon electrodes to the anaerobic redox catalysis of glucose oxidase. The electrodes are modified with ferrocene either directly, by using (1 ,l'-ferrocenediyl)dichlorosilane, or via an underlying polypyrrole film. Light-activated catalytic waves are detected in both cases in the presence of glucose oxidase and glucose, thereby demonstrating rapid heterogeneous kinetics between the covalently immobilized mediator and glucose oxidase. A glucose-sensitive electrode is made by simple adsorption of glucose oxidase from an aqueous buffered solution into the surface film.

Use of polymer films in amperometric biosensors

“…An innovative approach that employed a lpyrrole film-covered semiconductor electrode in the construction of a glucose biosensor was described by Dicks and co-workers [44]. Electropolymerization of the pyrrole film from acetonitrile solution onto the silicon wafer electrodes thalt had been modified with N-[3-(trimethoxysilylpropylpyrrole)] 'was performed at f0.75V (vs. SCE).…”

Biomolecular immobilization on conducting polymers for biosensing applications

“…But this process has some known disadvantages like binding forces can change with pH, adsorption is limited to one monolayer on the polymer surface hence amount of enzyme incorporated is very small [76]. Also, since biomaterial is immobilized on the outer layer of conducting polymer, it gets leached out into sample solution during measurements.…”

Vinylferrocene copolymers based biosensors for phenol derivatives

“…17 Moreover, The use of a redox polymer as mediator results in more stable biosensors since leaking of the mediator from the electrode is minimized and higher and faster responses are observed due to the proximity of enzyme and mediator. 17 Covalent linking of enzyme can be carried out by derivatization of polymer layers previously electrodeposited with mediators 18 or by synthesis of monomer derivatives substituted with mediator molecules from which the conducting/non-conducting polymer functionalized with electroactive unit is electro-synthesized. 19 -21 For example, copolymerization of thiophene with Os(bipy) 2 pyCl 96 www.soci.org F Dursun et al…”