The Application of Fish Collagen to Dental and Hard Tissue Regenerative Medicine

Hayashi, Yoshihiko; Ikeda, Takeshi; Yamada, Sōichiro; Koyama, Zenya; Yanagiguchi, Kajiro

doi:10.1007/978-1-4614-9590-1_22

Cited by 4 publications

(4 citation statements)

References 40 publications

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“…Several previous studies have demonstrated that the amino acid composition of fish collagen is similar to that of mammalian collagen [4]. For example, glycine is the most abundant amino acid, accounting for more than 30% of all amino acids.…”

Section: Introductionmentioning

confidence: 89%

The Characterization of Fish (Tilapia) Collagen Sponge as a Biomaterial

Yamamoto

Yoshizawa

Yanagiguchi

et al. 2015

International Journal of Polymer Science

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For scaffold manufacturing, the utility of bioactive natural organic materials derived from marine products is useful and indispensable as an alternative to bovine collagen. The weakest feature of fish collagen for scaffold application is its low degeneration temperature (Td), indicating poor stability of fish collagen in mammalsin vivo. We have focused on the tropical fish tilapia as a candidate for generating a clinical scaffold. The aim of this study was to confirm theTdof tilapia type I atelocollagen (TAC) for biomedical application. Furthermore, the physical and structural properties were investigated and evaluated as a scaffold on a sponge form. Different concentrations {0.5%, 1.0%, and 2.0% (v/v)} of TAC solution were analyzed. Differential scanning calorimetry showed that theTdof TAC was 35-36°C. The scanning electron microscopy results indicated that the pore size (90–160 μm) of TAC sponges is acceptable for cell proliferation. The tensile strength of porous sponges was in the range of 0.01–0.07 MPa. These findings indicate that the TAC sponge prepared from tilapia is one of candidates as a scaffold. The 1.0% (v/v) concentration of TAC solution is especially recommended to be advantageous for preparing and handling the solution and for sponge formation.

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Section: Introductionmentioning

confidence: 89%

The Characterization of Fish (Tilapia) Collagen Sponge as a Biomaterial

Yamamoto

Yoshizawa

Yanagiguchi

et al. 2015

International Journal of Polymer Science

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“…Marine collagen is also widely used in dentistry [12,101], generally as membrane, bone graft materials, an agent for local delivery and a hemostatic agent. In particular, the membranes are used in periodontal and implant therapy to guide soft tissue regeneration and inhibit the rapid regrowth of skin when implanting bone which takes longer to generate.…”

Section: Biomedical and Pharmaceutical Applications Of Collagenmentioning

confidence: 99%

Marine Collagen from Alternative and Sustainable Sources: Extraction, Processing and Applications

et al. 2020

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Due to its unique properties, collagen is used in the growing fields of pharmaceutical and biomedical devices, as well as in the fields of nutraceuticals, cosmeceuticals, food and beverages. Collagen also represents a valid resource for bioplastics and biomaterials, to be used in the emerging health sectors. Recently, marine organisms have been considered as promising sources of collagen, because they do not harbor transmissible disease. In particular, fish biomass as well as by-catch organisms, such as undersized fish, jellyfish, sharks, starfish, and sponges, possess a very high collagen content. The use of discarded and underused biomass could contribute to the development of a sustainable process for collagen extraction, with a significantly reduced environmental impact. This addresses the European zero-waste strategy, which supports all three generally accepted goals of sustainability: sustainable economic well-being, environmental protection, and social well-being. A zero-waste strategy would use far fewer new raw materials and send no waste materials to landfills. In this review, we present an overview of the studies carried out on collagen obtained from by-catch organisms and fish wastes. Additionally, we discuss novel technologies based on thermoplastic processes that could be applied, likewise, as marine collagen treatment.2 of 23 protective colloid functions and film-forming capacity. Also, collagen is a good surface-active agent, with its ability to penetrate lipid-free interfaces [2].Collagen can be utilized in a variety of applications because of its biocompatibility and excellent degradability [3,4]. Furthermore, it is known that collagen is a molecule with weak immunogenicity, which decreases the possibilities of rejection when it is ingested or injected into a different body. Although this molecule has already low antigenicity, this property can be enhanced by modifying it to suppress any immune response [5,6]. Additionally, collagen peptides and gelatin (denatured collagen) have been widely utilized in different fields such as food, medicine, cosmetics, leather and film industries, diagnostic imaging, and therapeutic delivery [7].For many years, most available collagen was extracted from discards from the bovine and porcine processing industries, but during the last few decades the use of collagen from these sources has been limited. The use of porcine and bovine-derived products is occasionally prevented by dietary regimes, due to specific needs or personal choices. It is forbidden by religious constraints, to Muslims, Hindus and Jews who make up 38.4% of the global population [8]. Moreover, the use of bovine-derived products became a concern for a wider section of the population during the bovine spongiform encephalopathy (BSE), transmissible spongiform encephalopathy (TSE) and foot-and-mouth disease (FMD) crises that occurred over the last few decades in all areas of the world, mostly in the United Kingdom and Asia. Bovine-derived products might be a vehicle of transmission of these diseases ...

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“…The enzymatic digestion time for atelotreatment and the elimination of enzyme in TAC are controlled to a clinical level (personal communication). The preliminary experimental data concerning pulp regeneration [24] using TAC as a scaffold revealed no rejection reaction on the newly regenerated pulp tissue in dogs (unpublished data). These findings suggest that TAC may be useful for clinical application after further animal experiments.…”

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confidence: 92%

“…The biodegradation of collagen is closely linked to and dependent on the constitution of amino acids in a given species. Compared to bovine collagen, the contents of methionine, isoleucine, leucine, hydroxylysine, and lysine are lower in TAC [24]. As these amino acids are related to the collagen stiffness, the tilapia collagen can be easily degraded in vivo.…”

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confidence: 96%

Fish Collagen Promotes the Expression of Genes Related to Osteoblastic Activity

Capati

Nakazono

Yamamoto

et al. 2016

International Journal of Polymer Science

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Tilapia type I atelocollagen (TAC) is a strong candidate for clinical application as its biological scaffold due to a high degeneration temperature and biologically safe properties. The aim of this study was to confirm the biological effects of TAC in vitro on osteoblastic cells, simulating its clinical application. The proliferation and differentiation of typical preosteoblasts, MC3T3-E1 cells, were investigated using a microarray analysis, staining assay for mineralization, and real-time PCR analysis of the expression of mineralization-related genes. The mRNA expression of 10 genes involved in proliferation and differentiation increased after 3-day culture on an TAC gel, with an average balanced score ratio exceeding 1.5 compared to the control. After two weeks of culture, all three experimental groups showed stronger alkaline phosphatase staining than after one week. The genes expression of alkaline phosphatase, osteocalcin, and bone sialoprotein increased under the experimental conditions. The gene expression of osteopontin did not increase, and no statistical differences were noted among the three experimental groups. The present and previous findings suggest that TAC is not only a suitable alternative to collagen products originating from mammals but also a novel biomaterial with cell differentiation ability for regenerative medicine.

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The Application of Fish Collagen to Dental and Hard Tissue Regenerative Medicine

Cited by 4 publications

References 40 publications

The Characterization of Fish (Tilapia) Collagen Sponge as a Biomaterial

The Characterization of Fish (Tilapia) Collagen Sponge as a Biomaterial

Marine Collagen from Alternative and Sustainable Sources: Extraction, Processing and Applications

Fish Collagen Promotes the Expression of Genes Related to Osteoblastic Activity

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