The Application of Fungal Enzymes in Flax Retting and the Properties of an Extracellular Polygalacturonase From Penicillium Capsulatum

Gillespie, A.-M.; Keane, D.; Griffin, Tadhg; Tuohy, M.; Donaghy, John; Haylock, R. W.; Coughlan, Michael P.

doi:10.1016/b978-0-409-90192-4.50023-6

Cited by 9 publications

(7 citation statements)

References 13 publications

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“…Three kinds of enzyme activity before treatment by Ca 2+ and after were detected by previous report (Gillespie et al, 1990). Enzyme assays were all performed in triplicate and mean values were provided.…”

Section: Enzyme Assaysmentioning

confidence: 99%

“…Pure bacterial cultures have been shown to ret flax fibers at higher yield and quality than did the natural microflora (Donaghy, Boomer, & Haylock, 1992). Commercial enzyme preparations have also been tried for retting flax (Sharma, 1987a(Sharma, , 1987b Gillespie suggested that enzymatic retting may be faster and more reproducible than traditional methods and may provide spinners with fibers of more uniform and higher quality (Gillespie et al, 1990). Saleem, Rennebaum, Pudel, and Grimm (2008) showed that pectinase improved mechanical characteristics of reinforced thermoplastic composites hemp (Cannabis sativa L.) fibers (Saleem et al, 2008).…”

Section: Introductionmentioning

confidence: 98%

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Degumming of ramie bast fibers by Ca²⁺-activated composite enzyme

Qiang

Yan

2013

Journal of The Textile Institute

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In recent years, special attention has been paid to the application of biotechnology in the textile industry. In this study, composite enzyme (contained pectatelyase/hemicellulase/laccase) was employed to degum the ramie bast successfully. Interestingly, the activities of enzymes were enhanced by promotion of Ca 2+ activated remarkably. The test of degummed ramie fiber performance demonstrated that fiber fineness, breaking strength, whiteness, and residual gum of fibers have greatly improved. Further, the structure and morphous of the fibers before and after degumming was examined and analyzed. The results showed that the gum, hemicellulose, and lignin were removed effectively and treated fibers had the typical cellulose I structure suitable for direct textile and other applications. These outcomes suggested that Ca 2+ -activated composite enzyme could degum the gum of ramie bast effectively, which provided a method to improve the quality of ramie fibers and a theoretical basis for flax degumming technique on an environmentally-friendly basis.

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Section: Enzyme Assaysmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Degumming of ramie bast fibers by Ca²⁺-activated composite enzyme

Qiang

Yan

2013

Journal of The Textile Institute

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“…Pectate lyase enzymes assist in the maceration of plant tissues by degrading the pectin located in the middle lamella and in the primary cell wall of higher plants [4]. They are involved in the retting and degumming of jute, Xax, hemp, and ramie bast Wbres [5][6][7][8]. Cellulose Wbres obtained from ramie are considered the longest, strongest, and silkiest plant Wbers.…”

Section: Introductionmentioning

confidence: 99%

Large-scale degumming of ramie fibre using a newly isolated Bacillus pumilus DKS1 with high pectate lyase activity

Basu¹,

Saha²,

Chattopadhyay³

et al. 2008

J Ind Microbiol Biotechnol

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A combined (enzymatic and chemical) process using a Bacillus pumilus strain (DKS1), isolated from the soil, was used to degum ramie bast fibres. After 24 h of incubation with the isolated pectinolytic strain using a low-cost medium, the weight loss of the ramie fibre was found to be 25% under small scale. High activity of pectate lyase was detected in the culture supernatants; 400 kg of ramie fibres was degummed with 24% weight loss in large-scale degumming under field conditions. No cellulase activity was found. Microbial intervention followed by mild (0.1%) alkali treatment showed high percentage of weight loss from the ramie fibre. Bacterial degumming followed by chemical treatment resulted in an increase of single fibre tenacity (cN/tex) by more than 20.81% as compared to non-degummed (decorticated) fibre samples. Scanning electron micrographs (SEM) and fluorescence microscope showed that after Bacillus pumilus DKS1 treatment the surface of the decorticated ramie fibre becomes very smooth. These results indicate the process provides an economical and eco-friendly method for the small scale as well as large-scale degumming of decorticated ramie fibre. This study has great relevance to the textile as well as paper industry.

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“…The conventional degumming of bast fibres method is undesirable due to its adverse effects on the environment . The enzymatic retting of bast fibres has been suggested as faster and easily reproducible . Recently, the degumming of ramie fibres has been demonstrated by using recombinant enzymes .…”

Section: Introductionmentioning

confidence: 99%

Bio‐scouring of cotton fabric and enzymatic degumming of jute fibres by a thermo‐alkaline recombinant rhamnogalacturonan lyase, ctrglf from Clostridium thermocellum

2018

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In this study, the use of recombinant rhamnogalacturonan lyase CtRGLf in bio-scouring of cotton and enzymatic degumming jute was evaluated. The best concentration for bio-scouring of cotton and degumming of jute was found to be 12 mg/mL (3.2 U/mL) of crude CtRGLf. Cotton fabric treated with 12 mg/mL (3.2 U/mL) of crude CtRGLf took 30 s to absorb a drop of water while the CtRGLf untreated fabric took 40 min. The crude CtRGLf reduced the water absorption time of cotton fabric by 98 % after 45 min of treatment. FESEM images showed that crude CtRGLf (12 mg/mL; 3.2 U/mL), effectively removed the waxy compounds from the surface of jute fibres after 1 h of treatment. These results showed that CtRGLf is a potentially important enzyme for the textile industry.

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The Application of Fungal Enzymes in Flax Retting and the Properties of an Extracellular Polygalacturonase From Penicillium Capsulatum

Cited by 9 publications

References 13 publications

Degumming of ramie bast fibers by Ca²⁺-activated composite enzyme

Degumming of ramie bast fibers by Ca²⁺-activated composite enzyme

Large-scale degumming of ramie fibre using a newly isolated Bacillus pumilus DKS1 with high pectate lyase activity

Bio‐scouring of cotton fabric and enzymatic degumming of jute fibres by a thermo‐alkaline recombinant rhamnogalacturonan lyase, ctrglf from Clostridium thermocellum

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The Application of Fungal Enzymes in Flax Retting and the Properties of an Extracellular Polygalacturonase From Penicillium Capsulatum

Cited by 9 publications

References 13 publications

Degumming of ramie bast fibers by Ca2+-activated composite enzyme

Degumming of ramie bast fibers by Ca2+-activated composite enzyme

Large-scale degumming of ramie fibre using a newly isolated Bacillus pumilus DKS1 with high pectate lyase activity

Bio‐scouring of cotton fabric and enzymatic degumming of jute fibres by a thermo‐alkaline recombinant rhamnogalacturonan lyase, ctrglf from Clostridium thermocellum

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Degumming of ramie bast fibers by Ca²⁺-activated composite enzyme

Degumming of ramie bast fibers by Ca²⁺-activated composite enzyme