The application of high-density genetic maps of rye for the detection of QTLs controlling morphological traits

Abstract

The development of genetic maps is, nowadays, one of the most intensive research activities of plant geneticists. One of the major goals of genome mapping is the localisation of quantitative trait loci (QTLs). This study was aimed at the identification of QTLs controlling morphological traits of rye and comparison of their localisation on genetic maps constructed with the use of genetically different germplasms. For QTL analyses, two high-density consensus maps of two populations (RIL-S and RIL-M) of recombinant inbred lines (RIL) were applied. Plant height (Ph), length of spikes (Sl) and the number of spikelets per spike (Sps) were studied in both populations. Additionally, the number of kernels per spike under isolation (Kps), the weight of kernels per spike (Kw) and thousand kernel weight (Tkw) were assessed in the RIL-M population. Except for Tkw, the majority of the traits were correlated to each other. The non-parametric Kruskal–Wallis (K-W) test and composite interval mapping (CIM) revealed 18/48 and 24/18 regions of rye chromosomes engaged in the determination of Ph, Sl and Sps in the RIL-S and RIL-M populations, respectively. An additional 18/15 QTLs controlling Kps, Kw and Tkw were detected on a map of the RIL-M population. A numerous group of QTLs detected via CIM remained in agreement with the genomic regions found when the K-W test was applied. Frequently, the intervals indicated by CIM were narrower.Electronic supplementary materialThe online version of this article (doi:10.1007/s13353-013-0186-5) contains supplementary material, which is available to authorized users.

Analysis of the impact of drought on selected morphological, biochemical and physiological traits of rye inbred lines

“…These lines are partly related, but they differ genetically (Myśków et al 2001;Milczarski et al 2011). RIL-M is a mapping population obtained in ZUT, used to obtain a high-density genetic map of rye (Milczarski et al 2011) and analysis of QTL controlling some morphological traits (Myśków et al 2014), earliness and pre-harvest sprouting (Myśków et al 2012).…”

DArT MARKERS LINKED WITH GENES CONTROLLING RESTORATION OF MALE FERTILITY IN HYBRID RYE CULTIVARS WITH IMPROVED POLLEN SHEDDING

“…Recently, several high-throughput genotyping methods significantly accelerating genetic analyses of numerous crops were developed. Among them, the diversity arrays technology (DArT) was successfully applied in rye research (Bolibok-Brągoszewska et al 2009;Milczarski et al 2011;Stojałowski et al 2011Stojałowski et al , 2015Myśków et al 2014;Gawroński et al 2016;Myśków and Stojałowski 2016). Therefore, in this study we applied the DArT technology to verify the Rfp1 gene efficacy in restoration of male fertility in PollenPlus® cultivars.…”

Genetic mapping of the ScHd1 gene in rye and an assessment of its relationship with earliness per se and plant morphology

“…A map used for the map position of Hd1 in rye was previously used for the localisation of the eps loci and of QTLs for different morphological traits such as PHS, AA, Ph, Sl, Sps, Kps, Kw and Tkw (Myśków et al 2012 , 2014 ). Additionally, the spike density coefficient (Ct) was calculated from the formula: 10 cm × Sps/Sl and collected data were included for further analysis.…”

“…However, the relationship of this marker with the phenotypic segregation of eps was shown to be statistically significant in two years out of a four-year research study, using the Kruskal–Wallis test. The non-parametric Kruskal–Wallis test frequently leads to different results than the CIM analysis (Myśków et al 2014 ) and is less accurate with respect to QTL mapping; however, it is a simple method of marker identification that can be used for the selection of an evaluated trait. It also enables the evaluation of a phenotypic effect that can be obtained using a certain selection marker.…”