The Application of Hollow Fiber Cartridge in Biomedicine

Abstract

The hollow fiber cartridge has the advantages of good semi-permeability, high surface area to volume ratio, convenient operation, and so on. Its application in chemical analysis, drug in vitro experiment, hemodialysis, and other fields has been deeply studied. This paper introduces the basic structure of hollow fiber cartridge, compares the advantages and disadvantages of a hollow fiber infection model constructed by a hollow fiber cartridge with traditional static model and animal infection model and introduces its application in drug effects, mechanism of drug resistance, and evaluation of combined drug regimen. The principle and application of hollow fiber bioreactors for cell culture and hollow fiber dialyzer for dialysis and filtration were discussed. The hollow fiber cartridge, whether used in drug experiments, artificial liver, artificial kidney, etc., has achieved controllable experimental operation and efficient and accurate experimental results, and will provide more convenience and support for drug development and clinical research in the future.

Pharmacodynamics of zoliflodacin plus doxycycline combination therapy against Neisseria gonorrhoeae in a gonococcal hollow-fiber infection model

“…Dual antimicrobial therapy can be a way to preserve an antibiotic and to prolong its usefulness; however, to evaluate if the combination of antibiotics is beneficial, if there is a synergistic effect or, at a minimum, an additive interaction so that the bacterial killing is enhanced and the likelihood of suppression of resistance is increased, various properties of the drug need to be considered. HFIM allows to alter the antimicrobial concentration, simulate various dosing regimens and different distribution schemes, single or multiple doses, and also simulate and combine antibiotics with differences in half-life, and all at the same time monitoring the efficacy in bacterial killing and the development of resistant subpopulations during treatment under the different administration schemes all to evaluate the best overall treatment effect ( Nielsen and Friberg, 2013 ; Lodise et al, 2020 ; Hou et el., 2022 ). There are limitations linked to HFIM to be considered when interpreting these findings.…”

Extracellular Vesicles as Next-Generation Diagnostics and Advanced Therapy Medicinal Products

“…Notably, the hollow-fiber bioreactor is widely used for EV production. This system is composed of thousands of parallel hollow fibers whose membrane supports cell attachment and the transport of small molecules (nutrients) from the fiber lumen to the extra-capillary space where the cell mass resides [ 70 ]. Furthermore, flow control systems continuously deliver fresh or recirculated cell culture medium to the lumen of the fibers to support cell growth, while the harvesting of EV-conditioned medium from the extra-capillary space is performed at specific time intervals.…”

Engineering cell-derived extracellular matrix for peripheral nerve regeneration

“…Hollow fiber bioreactors are particularly noteworthy for their cylindrical compartments packed with porous fibers that allow for nutrient exchange and waste removal, simulating the capillary structure of blood vessels. The large surface area of this system allows high cell densities to be achieved, making them a promising option for cd-ECM production [ 214 , 215 ]. Microcarrier systems are another scalable solution, providing an expanded surface for cell attachment and growth compared to traditional 2D platforms.…”