The application of in vitro cytotoxic reactions to clinical diagnosis of food allergy

Intestinal Assimilation of Intact Peptides and Proteins From the Diet‐a Neglected Field?

“…The appearance of intact polypeptides in plasma ultrafiltrates after ingestion of gliadin by coeliac patients has been reported by Kowlessar et al ( I 970) -see also Klosse et al (1972). Furthermore, gluten has recently been shown to possess lectin-like activity (Kottgen et al., 1982), a finding that is consistent with the hypothesis of Weiser & Douglas (1976) implicating lectins* in the aetiology of coeliac disease; this property may confer membrane-toxicity and may promote endocytosis, and it (rather than an immune response) may be responsible for a positive response to the Bryans's 'cytotoxic' test (Bryan & Bryan, 1960Black, 1956). Peptides derived by peptic digestion of gluten can exert opiate-like activity in vitro (Klee & Zioudrou, 1980;.…”

“…While an involvement of gliadin in coeliac disease is certain, the evidence for its involvement in schizophrenia is undoubtedly equivocal and the interpretation of any such epidemiological or statistical association is fraught with hazard ; therefore there rightly is considerable scepticism about any possible association between dietary gluten and the incidence of schizophrenia (e.g. of Bryan & Bryan (1960, but corresponding data on healthy control subjects were not provided. On balance, the studies suggest that some schizophrenic patients do respond beneficially to gluten exclusion (which must be prolonged) although many, probably the majority, do not (see also Cooke & Holmes, 1983).…”

Immunoglobulin E in Mucoid Secretory Otitis Media

“…The cytotoxic test as developed by Bryan and Bryan [1960] was also performed for 12 common foods [Ruokonen, 1982]. Reactions classified as moderate or strong were regarded as indications for further testing.…”

Management of diarrhoeic type of irritable bowel syndrome with exclusion diet and disodium cromoglycate

“…The positivity was established on the basis of cellular modifications observed when the suspension containing 15 to 20 neutrophils per field was placed on the previously prepared glass slides and examined with an apochromatic 60 power high dry lens. All glasses were meticulously cleaned and siliconized as described by Bryan and Bryan [8]. The results were graded + (in presence of swollen cells), ++ (vacuolated cells), and +++ (fragmented cells) [8].…”

“…All glasses were meticulously cleaned and siliconized as described by Bryan and Bryan [8]. The results were graded + (in presence of swollen cells), ++ (vacuolated cells), and +++ (fragmented cells) [8].…”