The Application of Matrix-Assisted Laser Desorption/Ionisation Time of Flight Mass Spectrometry to Profile the Surface of Intact Bacterial Cells

Shah, Haroun N.; Keys, Carrina J.; Gharbia, Saheer E.; Ralphson, Kathryn; Trundle, Frank; Brookhouse, Ian; Claydon, Martin A.

doi:10.3402/mehd.v12i4.8197

Cited by 8 publications

(5 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The peaks obtained from different microbial strains may be chemically different, as the cell surface molecules vary from species to species. For example Shah et al (2000) observed the differences in ionization of surface molecules between gram-positive and gramnegative bacteria. These ionized molecules may be mixture of small peptides, teichoic acids, oligosaccharides and lipids.…”

Section: Resultsmentioning

confidence: 95%

An attempt at taxonomical characterization of some Rhizobial species by intact cell MALDI mass spectrometry

Prabhu

Kulkarni

Parasharami

et al. 2006

World J Microbiol Biotechnol

View full text Add to dashboard Cite

In the present study an attempt was made to exploit the benefit of intact cell MALDI mass spectrometry (ICM-MS) in bringing out similarities and differences among some Rhizobium species and a species of Agrobacterium based on specific mass:charge (m/z) values. Rhizobium species isolated from the root nodules of selected leguminous plants were analysed by ICM-MS. The spectra were acquired in the range of 500-10,000 Da yielding several peaks specific to each species. The peaks obtained corresponded to the respective bacterial cell surface molecules which were desorbed during matrix-assisted laser desorption ionization. The number of peaks were more in the range of 500-1200 Da. Dice similarity coefficient analysis of m/z values indicated that Rhizobium species isolated from Trigonella foenum-graecum and Pisum arvense showed more similarity than any other species. Agrobacterium species did show a few common m/z values in comparison with other Rhizobium species. This clearly shows that Agrobacterium is closely related to Rhizobium. Eventually, ICM-MS technique offers clear, distinct, and consistent results for replicates, in less than an hour's time, therefore this technique has high potential in molecular taxonomy.

show abstract

Section: Resultsmentioning

confidence: 95%

An attempt at taxonomical characterization of some Rhizobial species by intact cell MALDI mass spectrometry

Prabhu

Kulkarni

Parasharami

et al. 2006

World J Microbiol Biotechnol

View full text Add to dashboard Cite

show abstract

“…Consequently, it is essential to establish that the process enables retention of microbial expression and cellular properties. Previous studies have confirmed that phenotypic and morphological characteristics remain unchanged (Shah et al, 2000(Shah et al, , 2002. However, such methods lack the resolution to identify subtle modification.…”

Section: Resultsmentioning

confidence: 98%

Assessment of the stability of cell-surface components of microorganisms by MALDI-TOF-MS following preservation on lenticule discs

Shah¹,

Molenaar

Rajakaruna³

et al. 2008

FEMS Microbiology Letters

Self Cite

View full text Add to dashboard Cite

Strains representing the species Campylobacter coli, Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa, Salmonella enterica, and Staphylococcus aureus were randomly selected to assess the consistency of cells preserved on lenticule discs to those archived in traditional freeze-dried ampoules. Each matched pair was cultured using identical conditions and analysed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MS) to profile the surface-associated molecules of the cells. In addition, the cytosolic/membrane-bound proteins of C. coli and S. aureus strains were further analysed by surface-enhanced laser desorption/ionization time-of-flight MS. The mass spectral profiles in all cases showed a high degree of concordance between cells preserved by both methods and suggest that the properties of cells preserved on lenticule disc are consistent with those archived by the traditional method of freeze-drying.

show abstract

“…Several trials of various technologies and microbial targets have been investigated (see e.g. Shah et al, 2000Shah et al, , 2002 and a database of some 5000 spectra has been developed (Keys et al, 2004). This initial approach was based upon the mass spectral profiles derived from the surface molecules of intact cells and when applied to clinical samples resulted in differential success in their universal application to bacterial identification.…”

Section: Recent Methods Of Characterizing Bacteroides Speciesmentioning

confidence: 99%

The GenusBacteroides

Shah¹,

Jacinto²,

Ahmod³

et al. 2008

Encyclopedia of Life Sciences

View full text Add to dashboard Cite

The genus Bacteroides has been described over 100 years and comprises a group of small nonmotile, nonsporing, Gram‐negative rods; they are obligate anaerobes and form a major part of the bacterial flora of the human intestinal tract. Poor circumscription of this group has led to the genus accumulating a large and diverse collection of species that only superficially resembles this description but could not be accommodated elsewhere. Based largely on chemotaxonomic and genetic criteria the genus was reclassified (Shah and Collins, 1989) to encompass the type species Bacteroides fragilis and members of the ‘ B. fragilis group’. This more restricted definition, spurred on mainly by 16S rRNA (ribosomal ribonucleic acid ), has encouraged more in‐depth analysis of the colonic flora and a large number of new species have been described. Full genomes of four species have been completed. Such studies are providing a better basis for elucidating the biological role of specific species within the intestinal tract and their involvement in a variety of physiological and cellular processes.

show abstract

The Application of Matrix-Assisted Laser Desorption/Ionisation Time of Flight Mass Spectrometry to Profile the Surface of Intact Bacterial Cells

Cited by 8 publications

References 5 publications

An attempt at taxonomical characterization of some Rhizobial species by intact cell MALDI mass spectrometry

An attempt at taxonomical characterization of some Rhizobial species by intact cell MALDI mass spectrometry

Assessment of the stability of cell-surface components of microorganisms by MALDI-TOF-MS following preservation on lenticule discs

The GenusBacteroides

Contact Info

Product

Resources

About