The Application of Mechanical Stimulations in Tendon Tissue Engineering

Sheng, Renwang; Jiang, Yujie; Backman, Ludvig J.; Zhang, Wei; Chen, Jialin

doi:10.1155/2020/8824783

Cited by 31 publications

(33 citation statements)

References 88 publications

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“…Although stem cells derived from bone marrow (BMSCs), adipose tissue (ASCs) and tendon/ligament tissues (TDSCs) have shown their efficacy as seed cells in tendon tissue engineering [27 , 28] , PDLSCs could be another good choice in spite that their potential in promoting tendon repair and regeneration should be further explored. To be a seed cell for tissue engineering application, the first important concern is accessibility.…”

Section: Discussionmentioning

confidence: 99%

The application of human periodontal ligament stem cells and biomimetic silk scaffold for in situ tendon regeneration

Chen

Sheng

et al. 2021

Stem Cell Res Ther

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Background With the development of tissue engineering, enhanced tendon regeneration could be achieved by exploiting suitable cell types and biomaterials. The accessibility, robust cell amplification ability, superior tendon differentiation potential, and immunomodulatory effects of human periodontal ligament stem cells (hPDLSCs) indicate their potential as ideal seed cells for tendon tissue engineering. Nevertheless, there are currently no reports of using PDLSCs as seed cells. Previous studies have confirmed the potential of silk scaffold for tendon tissue engineering. However, the biomimetic silk scaffold with tendon extracellular matrix (ECM)-like structure has not been systematically studied for in situ tendon regeneration. Therefore, this study aims to evaluate the effects of hPDLSCs and biomimetic silk scaffold on in situ tendon regeneration. Methods Human PDLSCs were isolated from extracted wisdom teeth. The differentiation potential of hPDLSCs towards osteo-, chondro-, and adipo-lineage was examined by cultured in different inducing media. Aligned and random silk scaffolds were fabricated by the controlled directional freezing technique. Scaffolds were characterized including surface structure, water contact angle, swelling ratio, degradation speed and mechanical properties. The biocompatibility of silk scaffolds was evaluated by live/dead staining, SEM observation, cell proliferation determination and immunofluorescent staining of deposited collagen type I. Subsequently, hPDLSCs were seeded on the aligned silk scaffold and transplanted into the ruptured rat Achilles tendon. Scaffolds without cells served as control groups. After 4 weeks, histology evaluation was carried out and macrophage polarization was examined to check the repair effects and immunomodulatory effects. Results Human PDLSCs were successfully isolated, and their multi-differentiation potential was confirmed. Compared with random scaffold, aligned silk scaffold had more elongated and aligned pores and promoted the proliferation and ordered arrangement of hPDLSCs. After implantation into rat Achilles tendon defect, hPDLSCs seeded aligned silk scaffold enhanced tendon repair with more tendon-like tissue formation after 4 weeks, as compared to the scaffold-only groups. Higher expression of CD206 and lower expression of iNOS, IL-1β and TNF-α were found in the hPDLSCs seeded aligned silk scaffold group, which revealed its modulation effect of macrophage polarization from M1 to M2 phenotype. Conclusions In summary, this study demonstrates the efficacy of hPDLSCs as seed cells and aligned silk scaffold as a tendon-mimetic scaffold for enhanced tendon tissue engineering, which may have broad implications for future tendon tissue engineering and regenerative medicine researches.

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Section: Discussionmentioning

confidence: 99%

The application of human periodontal ligament stem cells and biomimetic silk scaffold for in situ tendon regeneration

Chen

Sheng

et al. 2021

Stem Cell Res Ther

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“…Physical signals are equally a focus of research as moderate mechanical stimulation ensures maintenance of the natural phenotype of tissues [10]. Mechanical stimulation methods are therefore used to generate a specific tissue phenotype in TERM such as cartilage [11], tendon [12], or cardiac muscle tissue [13].…”

Section: Tissue Engineering and Regenerative Medicine (Term) 21 Fundamentals Of Tissue Engineering And Regenerative Medicinementioning

confidence: 99%

Isotropic and Anisotropic Scaffolds for Tissue Engineering: Collagen, Conventional, and Textile Fabrication Technologies and Properties

Tonndorf

Aibibu

Cherif

2021

IJMS

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In this review article, tissue engineering and regenerative medicine are briefly explained and the importance of scaffolds is highlighted. Furthermore, the requirements of scaffolds and how they can be fulfilled by using specific biomaterials and fabrication methods are presented. Detailed insight is given into the two biopolymers chitosan and collagen. The fabrication methods are divided into two categories: isotropic and anisotropic scaffold fabrication methods. Processable biomaterials and achievable pore sizes are assigned to each method. In addition, fiber spinning methods and textile fabrication methods used to produce anisotropic scaffolds are described in detail and the advantages of anisotropic scaffolds for tissue engineering and regenerative medicine are highlighted.

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“…In terms of strain a wide variety of stimulus regimes have been used, with a consensus that uniaxial strain is more beneficial than biaxial strain for myogenesis at strain amplitudes of 10-15% [95]. Additionally, mechanical loading should be applied on cells in a 3D environment as studies have shown that this mimics mechanical loading in natural systems [96]. Under appropriate conditions the 3D loading of cells allows them to recognize mechanical stimulation as cell-ECM interactions, which results in the precise differentiation desired in the reconstruction of native muscle [97].…”

Section: External Stimuli For Muscle Cell Differentiationmentioning

confidence: 99%

Systems for Muscle Cell Differentiation: From Bioengineering to Future Food

Lee¹,

Loh²,

Wan³

2021

Micromachines

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In light of pressing issues, such as sustainability and climate change, future protein sources will increasingly turn from livestock to cell-based production and manufacturing activities. In the case of cell-based or cultured meat a relevant aspect would be the differentiation of muscle cells into mature muscle tissue, as well as how the microsystems that have been developed to date can be developed for larger-scale cultures. To delve into this aspect we review previous research that has been carried out on skeletal muscle tissue engineering and how various biological and physicochemical factors, mechanical and electrical stimuli, affect muscle cell differentiation on an experimental scale. Material aspects such as the different biomaterials used and 3D vs. 2D configurations in the context of muscle cell differentiation will also be discussed. Finally, the ability to translate these systems to more scalable bioreactor configurations and eventually bring them to a commercial scale will be touched upon.

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The Application of Mechanical Stimulations in Tendon Tissue Engineering

Cited by 31 publications

References 88 publications

The application of human periodontal ligament stem cells and biomimetic silk scaffold for in situ tendon regeneration

The application of human periodontal ligament stem cells and biomimetic silk scaffold for in situ tendon regeneration

Isotropic and Anisotropic Scaffolds for Tissue Engineering: Collagen, Conventional, and Textile Fabrication Technologies and Properties

Systems for Muscle Cell Differentiation: From Bioengineering to Future Food

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