The Application of Metabolomics for the Study of Cereal Corn (Zea mays L.)

Ranilla, Lena Gálvez

doi:10.3390/metabo10080300

Cited by 31 publications

(14 citation statements)

References 118 publications

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“…As sessile organisms, plants have evolved various mechanisms to sense environmental temperature and adapt to natural 29,30 . Histone methylation also plays an important role in this [31][32][33] . Corn is native to South America 34 , is a warm and light-loving crop, it has high temperature requirements.…”

Section: Discussionmentioning

confidence: 99%

Effects of histone methylation modification on low temperature seed germination and growth of maize

Wan

Zhang

et al. 2023

Preprint

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Low temperature is a limiting factor of seed germination and plant growth. Although there is a lot information on the response of maize to low temperatures, there is still poorly description of how histone methylation affects maize germination and growth development at low temperatures. In this study, the germination rate and physiological indexes of wild-type maize inbred lines B73 (WT), SDG102 silencing lines (AS), SDG102 overexpressed lines (OE) at germination stage and seedling stage were measured under low temperature stress (4℃), and transcriptome sequencing was applied to analyze the differences of gene expression in panicle leaves among different materials. The results showed that the germination rate of WT and OE maize seeds at 4℃ was significantly lower than 25℃. The content of MDA, SOD and POD of 4℃ seeding leaves higher than contrast. Transcriptome sequencing results showed that there were 409 different expression genes (DEGs) between WT and AS, and the DEGs were mainly up-regulated expression in starch and sucrose metabolism and phenylpropanoid biosynthesis. There were 887 DEGs between WT and OE, which were mainly up-regulated in the pathways of plant hormone signal transduction, porphyrin and chlorophyll metabolism. This result could provide a theoretical basis for analyzing the growth and development of maize from the perspective of histone methylation modification.

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Section: Discussionmentioning

confidence: 99%

Effects of histone methylation modification on low temperature seed germination and growth of maize

Wan

Zhang

et al. 2023

Preprint

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“…Jigh-performance liquid chromatography (HPLC) was predominantly used to identify carotenoids [ 12 , 13 , 14 ] and polyphenols [ 15 , 16 ] in corn grains. A review by Ranilla (2020) summarized the application of metabolomics for the characterization of metabolites in corn grains and emphasized the importance of phenotype–genotype studies aimed to explore corn genetic diversity [ 17 ]. The application of electrospray ionization mass spectrometry (ESI–MS) in combination with HPLC is a cost-effective and statistically robust method for high-throughput phenotypic characterization of corn [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Spatial Distribution of Polyphenolic Compounds in Corn Grains (Zea mays L. var. Pioneer) Studied by Laser Confocal Microscopy and High-Resolution Mass Spectrometry

Razgonova

Zinchenko

Pikula

et al. 2022

Plants

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Desirable changes in the biochemical composition of food plants is a key outcome of breeding strategies. The subsequent localization of nutritional phytochemicals in plant tissues gives important information regarding the extent of their synthesis across a tissue. We performed a detailed metabolomic analysis of phytochemical substances of grains from Zea mays L. (var. Pioneer) by tandem mass spectrometry and localization by confocal microscopy. We found that anthocyanins are located mainly in the aleurone layer of the grain. High-performance liquid chromatography in combination with ion trap tandem mass spectrometry revealed the presence of 56 compounds, including 30 polyphenols. This method allows for effective and rapid analysis of anthocyanins by plotting their distribution in seeds and grains of different plants. This approach will permit a more efficient screening of phenotypic varieties during food plant breeding.

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“…Liquid chromatography coupled with mass spectrometry (LC–MS) of semi‐polar extracts provides information about specialized metabolites, for example, flavonoids, hydroxycinnamates and benzoxazinoids. Recently, such analytical approaches have been applied widely to cereals (Balmer, Flors, Glauser, & Mauch‐Mani, 2013; Gálvez, 2020; Khakimov, Bak, & Engelsen, 2014; Saia, Fragasso, De Vita, & Beleggia, 2019), including maize for studies on environmental effects (Sun et al, 2016; Tang et al, 2017). Besides primary metabolites (Cañas et al, 2017; Sun, Li, et al, 2016), maize leaf contains several families of specialized metabolites involved in stress responses, such as quinic acid derivatives (Cortés‐Cruz, Snook, & McMullen, 2003), flavone glycoside maysin (Rector, Liang, & Guo, 2003) and benzoxazinoids (Zhou, Richter, & Jander, 2018).…”

Section: Introductionmentioning

confidence: 99%

Maize metabolome and proteome responses to controlled cold stress partly mimic early‐sowing effects in the field and differ from those of Arabidopsis

Urrutia

Blein-Nicolas

Prigent

et al. 2021

Plant Cell & Environment

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In Northern Europe, sowing maize one-month earlier than current agricultural practices may lead to moderate chilling damage. However, studies of the metabolic responses to low, non-freezing, temperatures remain scarce. Here, geneticallydiverse maize hybrids (Zea mays, dent inbred lines crossed with a flint inbred line) were cultivated in a growth chamber at optimal temperature and then three decreasing temperatures for 2 days each, as well as in the field. Leaf metabolomic and proteomic profiles were determined. In the growth chamber, 50% of metabolites and 18% of proteins changed between 20 and 16 C. These maize responses, partly differing from those of Arabidopsis to short-term chilling, were mapped on genome-wide metabolic maps. Several metabolites and proteins showed similar variation for all temperature decreases: seven MS-based metabolite signatures and two proteins involved in photosynthesis decreased continuously. Several increasing metabolites or proteins in the growth-chamber chilling conditions showed similar trends in the early-sowing field experiment, including trans-aconitate, three hydroxycinnamate derivatives, a benzoxazinoid, a sucrose synthase, lethal leaf-spot 1 protein, an allene oxide synthase, several glutathione transferases and peroxidases. Hybrid groups based on field biomass were used to search for the metabolite or

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The Application of Metabolomics for the Study of Cereal Corn (Zea mays L.)

Cited by 31 publications

References 118 publications

Effects of histone methylation modification on low temperature seed germination and growth of maize

Effects of histone methylation modification on low temperature seed germination and growth of maize

Spatial Distribution of Polyphenolic Compounds in Corn Grains (Zea mays L. var. Pioneer) Studied by Laser Confocal Microscopy and High-Resolution Mass Spectrometry

Maize metabolome and proteome responses to controlled cold stress partly mimic early‐sowing effects in the field and differ from those of Arabidopsis

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