The application of molecular markers to the study and conservation of fish populations, with special reference to <i>Salmo</i>

Ferguson, A.; Taggart, John B.; Prodöhl, Paulo A.; McMeel, O. M.; Thompson, Chris; Stone, C.; McGinnity, P.; Hynes, Rosaleen

doi:10.1111/j.1095-8649.1995.tb06048.x

Cited by 191 publications

(144 citation statements)

References 75 publications

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“…Relationship among haplotypes and populations Despite the high levels of variation detected in the Control Region of higher vertebrates, D-loops of some fish species, including S. trutta appear to evolve at a lower rate than other regions of the mtDNA (Ferguson et a!., 1995). This finding is further supported in our studies.…”

Section: Discussionsupporting

confidence: 81%

Genetic differentiation and phylogenetic relationships among Greek Salmo trutta L. (brown trout) populations as revealed by RFLP analysis of PCR amplified mitochondrial DNA segments

1996

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The genetic differentiation and phylogenetic relationships of 13 populations (11 from Greece, one from Albania and one from France) of brown trout (Salmo trutta L.) were investigated at the mtDNA level. RFLP analysis of four segments of mitochondrial DNA (D-loop, cytochrome b and ND-5!6) amplified by PCR was used. Seven of 14 restriction endonucleases were found to detect variability in the ND-516 regions, whereas four and two out of 17 and 16 were polymorphic in the D-loop and cytochrome b, respectively. Eleven different haplotypes were observed. The observed interpopulation diversity was very high (mean = 1.65 per cent), whereas the intrapopulation diversity was low in most cases (mean =0.063 per cent). Five phylogenetic assemblages were identified. The results demonstrate that Greece is one of the regions where brown trout display very high levels of genetic diversity. Most populations were genetically very distinct, possessing private mtDNA genotypes. Therefore, they represent unique gene pools which may warrant individual recognition for conservation and management. The genetic relationships among populations suggested by mtDNA data were not in accordance with allozyme data. This study illustrates the importance of considering the historical biogeography of a species in order to understand its population genetic structure. It also reinforces the view that mtDNA analysis represents a powerful tool to study past and present demographic phenomena.

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Section: Discussionsupporting

confidence: 81%

Genetic differentiation and phylogenetic relationships among Greek Salmo trutta L. (brown trout) populations as revealed by RFLP analysis of PCR amplified mitochondrial DNA segments

1996

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“…In comparison with dominant markers (such as RAPDs), co-dominant genetic markers are very useful because of the information content of a single locus. Although allozyme markers have been found to be useful, microsatellites are superior considering the level of polymorphism and the information content of a single locus, and the sample requirement and sampling method (Ferguson et al 1995).…”

Section: Introductionmentioning

confidence: 99%

Microsatellite marker isolation and cultured strain identification in Carassius auratus gibelio

Guo

Gui

2008

Aquacult Int

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Microsatellites have become the preferred molecular markers for strain selection and genetic breeding in fish. In this study a total of 105 microsatellites were isolated and identified in gibel carp (Carassius auratus gibelio) by microsatellite sequence searches in GenBank and other databases and by screening and sequencing of positive clones from the genomic library enriched for AG and GATA repeats. Moreover, nineteen microsatellites were randomly selected to design locus-specific primer pairs, and these were successfully used to identify and discriminate different cultured strains of gibel carp including strains A, D, L, and F. Three different types of microsatellite pattern were distinguished by the number and length of fragments amplified from the 19 primer pairs, and some microsatellite primer pairs were found to produce different microsatellite patterns among strains and strain-specific fragments. In addition, some duplicated alleles were also detected in two microsatellite patterns. Therefore, the current study provides direct molecular markers to discriminate among different cultured strains for selective breeding and aquaculture practice of gibel carp.

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“…Such reductions may result in decreased fitness and the eventual extinction of populations (Fergunson et al, 1995). Thus, it is now widely appreciated that understanding patterns of genetic variation is critically important for the conservation of threatened species (Allnut et al, 2003).…”

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confidence: 99%

Genetic structure analysis of Eufriesea violacea (Hymenoptera, Apidae) populations from southern Brazilian Atlantic rainforest remnants

et al. 2005

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Random amplified polymorphic DNA (RAPD) markers were used to analyze the genetic structure of Eufriesea violacea populations in three fragments (85.47, 832.58 and 2800 ha) of Atlantic rainforest located in the north of the Brazilian state of Paraná. A total of twelve primers produced 206 loci, of which 129 were polymorphic (95% criterion). The proportions of polymorphic loci in each population ranged from 57.28% to 59.2%, revealing very similar levels of genetic variability in the groups of bees from each fragment. Unbiased genetic distances between groups ranged from 0.0171 to 0.0284, the smallest genetic distance occurring between bees from the two larger fragments. These results suggest that the E. violacea populations from the three fragments have maintained themselves genetically similar to native populations of this species originally present in northern Paraná.

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The application of molecular markers to the study and conservation of fish populations, with special reference to Salmo

Cited by 191 publications

References 75 publications

Genetic differentiation and phylogenetic relationships among Greek Salmo trutta L. (brown trout) populations as revealed by RFLP analysis of PCR amplified mitochondrial DNA segments

Genetic differentiation and phylogenetic relationships among Greek Salmo trutta L. (brown trout) populations as revealed by RFLP analysis of PCR amplified mitochondrial DNA segments

Microsatellite marker isolation and cultured strain identification in Carassius auratus gibelio

Genetic structure analysis of Eufriesea violacea (Hymenoptera, Apidae) populations from southern Brazilian Atlantic rainforest remnants

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