The application of paper chromatography to the study of steroid structure

Cathro, D. M.; Cameron, Jenny; Birchall, Kathleen

doi:10.1016/s0021-9673(00)99881-9

Cited by 9 publications

(3 citation statements)

References 7 publications

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“…Two other steroids that may be present in biological material, namely androst-4-ene-3,11,17trione (adrenosterone) and 17a-hydroxypregn-4ene-3,20-dione (17a-hydroxyprogesterone), behave chromatographically on paper in the same way as testosterone (Cathro, Cameron & Birchall, 1965). Adrenosterone was clearly separated from testosterone and androstenedione by gas-liquid chromatography on a column coated with the phase QF-1.…”

Section: Discussionmentioning

confidence: 99%

A method for the estimation of urinary testosterone

Ismail¹,

Harkness²

1966

Biochemical Journal

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1. A method has been developed for the estimation of testosterone in human urine by using acid hydrolysis followed by a quantitative form of a modified Girard reaction that separates a ;conjugated-ketone' fraction from a urine extract; this is followed by column chromatography on alumina and paper chromatography. 2. Comparison of methods of estimation of testosterone in the final fraction shows that estimation by gas-liquid chromatography is more reproducible than by colorimetric methods applied to the same eluates from the paper chromatogram. 3. The mean recovery of testosterone by gas-liquid chromatography is 79.5%, and this method appears to be specific for testosterone. 4. The procedure is relatively rapid. Six determinations can be performed by one worker in 2 days. 5. Results of determinations on human urine are briefly presented. In general, they are similar to earlier estimates, but the maximal values are lower.

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Section: Discussionmentioning

confidence: 99%

A method for the estimation of urinary testosterone

Ismail¹,

Harkness²

1966

Biochemical Journal

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“…The HPLC selectivity parameter a = k{jh{ (34) corresponds in TLC to ARm = log (k{¡k{). Several authors (35)(36)(37)(38)(39)(40)(41) have used this value in correlations with steroid structures, mainly for the evaluation of the contribution of a functional group and the selectivity of developing solvents. From eq 4, it is evident that An(AR°uoh -log us) (5) where 1 and 2 denote compounds which differ in molecular structure.…”

Section: Structure Of Steroids Frommentioning

confidence: 99%

Steroid structure and solvent composition in thin-layer chromatography

Lukić-Petrović¹,

Kolarov²,

Traljić³

et al. 1982

Anal. Chem.

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“…A number of color and fluorescence reactions, suitable for PC, have been described (1872), as well as some special radiochemical techniques (1676,1946,2023,2204). Additional work on the application of PC to structural analysis has been published, (268,506).…”

Section: Alkaloids Both Macekmentioning

confidence: 99%

Chromatography

Heftmanń¹

1966

Anal. Chem.

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The application of paper chromatography to the study of steroid structure

Cited by 9 publications

References 7 publications

A method for the estimation of urinary testosterone

A method for the estimation of urinary testosterone

Steroid structure and solvent composition in thin-layer chromatography

Chromatography

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