The Application of Plant Growth Regulators in Propagation of Dendrobium Orchid with Thin Cell Layer (TCL) Technique: A Review

Abstract

Plant growth regulators is one of the success factors for in vitro propagation. Plant growth regulators are organic compounds that are not nutrients and, in small amounts, promote, inhibit, or regulate physiological processes in plants. The function of growth regulators is to stimulate the growth of morphogenesis in cell, tissue and organ cultures. The most used plant growth regulators in tissue culture are auxins, cytokinins and gibberellins. One of technique for tissue culture is the thin cell layer technique. Thin Cell Layer (TCL) is a technique using small thin sections produced from organ pieces. TCL explants more effective than large conventional explants. Thin explants facilitate contact and diffusion of media into the tissue, better than thick explant slices. This literature review aims to gather information related to the application of plant growth regulators in propagation of Dendrobium orchid with thin cell layer (tcl) technique. A systematic Literature Review (SLR) uses in this paper. The growth regulators commonly used in Dendrobium orchid propagation using the TCL technique are auxins and cytokinins and the type of media that is often used in Dendrobium orchid propagation is Murashige and Skoog (MS). Protocorm Like Bodies (PLB) is the most potential explant in Dendrobium orchid propagation. The TCL technique both transverse and longitudinal has its own specificity with each type of explant used. TCL explants proved to be more effective than large conventional explants.

Shoot Induction of Dendrobium lasianthera J.J.Smith with Several Types of Cytokinins through In Vitro Culture

“…Some factors contributing to tissue culture techniques include seed maturity, genotype, media, growth regulators, carbohydrates, and explant type (Tan et al, 2013;Reddy et al, 2021). Cytokinin is one of the growth regulators commonly used in plant propagation, including orchids (Arli et al, 2023). Cytokinin stimulates cell division and shoot growth in tissue culture.…”