The Application of Quantitative Metabolomics for the Taxonomic Differentiation of Birds

Zelentsova, Ekaterina A.; Yanshole, Lyudmila V.; Tsentalovich, Yuri P.; Sharshov, Kirill; Yanshole, Vadim V.

doi:10.3390/biology11071089

Cited by 7 publications

(9 citation statements)

References 44 publications

(83 reference statements)

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“…The bird species and the number of individuals within each species used for metabolomic analysis as well as the territorial localization of the samples collection are shown in Table 1 . NMR-based metabolomic analysis of bird lenses was performed in the same way as described in 23 , and the complete set of obtained results is presented in Table S1 , where the metabolite concentrations in lenses are expressed in units of nmol per gram of the wet tissue. The data for M. migrans, P. cristatus, and C. livia are taken from 23 .…”

Section: Resultsmentioning

confidence: 99%

“…NMR-based metabolomic analysis of bird lenses was performed in the same way as described in 23 , and the complete set of obtained results is presented in Table S1 , where the metabolite concentrations in lenses are expressed in units of nmol per gram of the wet tissue. The data for M. migrans, P. cristatus, and C. livia are taken from 23 . The data on reduced NADH and oxidized NAD + forms of nicotinamide adenine dinucleotide are collected in a separate table (Table 2 ).…”

Section: Resultsmentioning

confidence: 99%

“…In our recent work 23 , we studied the metabolomic composition of lenses from 14 bird species, and detected unexpectedly high levels of NADH (β-Nicotinamide adenine dinucleotide reduced, Fig. 1 ) in lenses of two species, black kite ( Milvus migrans ) and great crested grebe ( Podiceps cristatus ).…”

Section: Introductionmentioning

confidence: 99%

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Nicotinamide adenine dinucleotide reduced (NADH) is a natural UV filter of certain bird lens

Osik

Zelentsova

Sharshov

et al. 2022

Sci Rep

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In this work, we for the first time report the identification of UV filters in the bird eye lens. We found that lenses of some raptors (black kite, common buzzard) and waterfowl (birds from Podicipedidae family) contain unusually high levels of reduced nicotinamide adenine dinucleotide (NADH)—a compound with high absorption in the UV-A range with a maximum at 340 nm. The lens metabolome of these birds also features an extremely low [NAD +]/[NADH] ratio. Chemometric analysis demonstrates that the differences between the metabolomic compositions of lenses with low and high NADH abundances should be attributed to the taxonomic features of bird species rather to the influence of the low [NAD +]/[NADH] ratio. We attributed this observation to the low metabolic activity in lens fiber cells, which make up the bulk of the lens tissue. Photochemical measurements show that properties of NADH as a UV filter are as good as that of UV filters in the human lens, including strong absorption in the UV-A spectral region, high photostability under both aerobic and anaerobic conditions, low yields of triplet state, fluorescence, and radicals under irradiation. Lenticular UV filters protect the retina and the lens from photo-induced damages and improve the visual acuity by reducing chromatic aberrations; therefore, the results obtained contribute to our understanding of the extremely high acuity of the raptor vision.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Nicotinamide adenine dinucleotide reduced (NADH) is a natural UV filter of certain bird lens

Osik

Zelentsova

Sharshov

et al. 2022

Sci Rep

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“…The dataset includes not only the final table with metabolite concentrations, but also all the raw NMR data and detailed protocols for sample preparation and data preprocessing. A part of this dataset formed the basis of a pilot study on the use of quantitative metabolomic profiling for taxonomic differentiation of species [1]. Also, analysis of a subset of this dataset made it possible to discover a new molecular ultraviolet filter in the bird eye lens -nicotinamide adenine dinucleotide reduced (NADH) [2].…”

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Quantitative Metabolomic Dataset of Avian Eye Lenses

Zelentsova¹,

Mariasina²,

Yanshole³

et al. 2023

Preprint

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Metabolomics is a powerful set of methods that uses analytical techniques to identify and quantify metabolites in biological samples, providing a snapshot of the metabolic state of a biological system. In medicine, metabolomics can help diagnose diseases, reveal molecular basis of a disease, and monitor treatment responses, while in agriculture, it can improve crop yields and plant breeding. However, animal metabolomics faces several challenges due to the complexity and diversity of animal metabolomes, the lack of standardized protocols, and the difficulty in interpreting metabolomic data. The current dataset includes quantitative metabolomic profiles of eye lens tissues from 26 bird species (111 specimens) that can aid researchers in developing new experiments, mathematical models, and integrations with other omics data. The dataset includes raw 1H NMR spectra, protocols for sample preparation, and data preprocessing with the final table containing information on 89 reliably identified and quantified metabolites. The dataset is quantitative, making it relevant for supplementing with new specimens or comparison groups, followed by data mining and expected new interpretations. It was obtained from bird specimens collected in compliance with ethical standards and reveals potential differences in metabolic pathways due to phylogenetic difference or environmental exposure.

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“…The dataset includes not only the final table with metabolite concentrations but also all the raw NMR data and detailed protocols for sample preparation and data preprocessing. A part of this dataset formed the basis of a pilot study on the use of quantitative metabolomic profiling for taxonomic differentiation of species [1]. Also, analysis of a subset of this dataset made it possible to discover a new molecular ultraviolet filter in the bird's eye lens-nicotinamide adenine dinucleotide reduced (NADH) [2].…”

mentioning

confidence: 99%

Quantitative Metabolomic Dataset of Avian Eye Lenses

Zelentsova,

Mariasina,

Yanshole

et al. 2023

Data

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View full text Add to dashboard Cite

Metabolomics is a powerful set of methods that uses analytical techniques to identify and quantify metabolites in biological samples, providing a snapshot of the metabolic state of a biological system. In medicine, metabolomics may help to reveal the molecular basis of a disease, make a diagnosis, and monitor treatment responses, while in agriculture, it can improve crop yields and plant breeding. However, animal metabolomics faces several challenges due to the complexity and diversity of animal metabolomes, the lack of standardized protocols, and the difficulty in interpreting metabolomic data. The current dataset includes quantitative metabolomic profiles of eye lenses from 26 bird species (111 specimens) that can aid researchers in developing new experiments, mathematical models, and integrating with other “-omics” data. The dataset includes raw 1H NMR spectra, protocols for sample preparation, and data preprocessing, with the final table containing information on the abundance of 89 reliably identified and quantified metabolites. The dataset is quantitative, making it relevant for supplementing with new specimens or comparison groups, followed by data mining and expected new interpretations. The data were obtained using the bird specimens collected in compliance with ethical standards and revealed potential differences in metabolic pathways due to phylogenetic differences or environmental exposure.

show abstract

The Application of Quantitative Metabolomics for the Taxonomic Differentiation of Birds

Cited by 7 publications

References 44 publications

Nicotinamide adenine dinucleotide reduced (NADH) is a natural UV filter of certain bird lens

Nicotinamide adenine dinucleotide reduced (NADH) is a natural UV filter of certain bird lens

Quantitative Metabolomic Dataset of Avian Eye Lenses

Quantitative Metabolomic Dataset of Avian Eye Lenses

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