The application of SFDI and LSI system to evaluate the blood perfusion in skin flap mouse model

Lyu, Lele; Kim, Hyeongbeom; Bae, Jong Seok; Hua, Cheng; Kim, Jie Hye; Kim, Eun‐Hee; Mo, Ji‐Hun; Park, Ilyong

doi:10.1007/s10103-021-03354-6

Cited by 5 publications

(5 citation statements)

References 32 publications

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“…Therefore, it can be concluded that LASCA has proved to be extremely useful in detecting the ischemic necrosis at the early stage. This observation correlates directly with the results of Lyu et al [26]. Lyu's research team used LASCA to assess the incidence of ischemic necrosis of flaps in a mouse model.…”

Section: Discussionsupporting

confidence: 81%

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Laser speckle contrast analysis (LASCA): a new device in the diagnosis and monitoring of surgical treatment of hidradenitis suppurativa

Gierek¹,

Bergler‐Czop²,

Słaboń³

et al. 2023

pdia

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Introduction: Laser speckle contrast analysis (LASCA) is a quite new and unique device which can find applications in multiple diagnostic approaches in dermatology and dermatosurgery. LASCA can be used in multiple approaches. We would like to make the world's first presentation of LASCA usefulness in hidradenitis suppurativa (HS) surgery based on a case series. Aim: To evaluate the usefulness of LASCA in the surgical treatment of HS. Material and methods: In HS surgical treatment at the Centre for Burns Treatment in Siemianowice Śląskie, Poland in 2019-2022, we performed standard preoperative, intraoperative and postoperative speckle laser examinations to assess the vascular perfusion of the surgical sites. The device used was Laser Speckle Contrast Analysis (Perimed AG). This study included 18 patients treated surgically at the Centre for Burns Treatment in Siemianowice Śląskie with specific LASCA findings. Results: As a result of the LASCA examination, we found 1) ischemia of the flap, and 2) local foci of HS, and we could also assess the healing process. Conclusions: LASCA is a great device with which we can evaluate wound healing after surgical procedures (STSG and skin local flaps). LASCA allows for early detection of post-operative complications like ischemia of the local skin flap.

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Section: Discussionsupporting

confidence: 81%

“…This observation correlates directly with the results of Lyu et al . [ 26 ]. Lyu’s research team used LASCA to assess the incidence of ischemic necrosis of flaps in a mouse model.…”

Section: Discussionmentioning

confidence: 99%

Laser speckle contrast analysis (LASCA): a new device in the diagnosis and monitoring of surgical treatment of hidradenitis suppurativa

Gierek¹,

Bergler‐Czop²,

Słaboń³

et al. 2023

pdia

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“…In our case, prior work in SFDI determined that optimal wavelengths for oxygenation imaging were close to 665 nm and 860 nm (Mazhar et al, 2010). Our group and others have translated similar technology to preclinical and clinical experiments (Gioux et al, 2011;Nadeau et al, 2013;Ponticorvo et al, 2013;Ghijsen et al, 2018;Schmidt et al, 2019;Weinkauf et al, 2019;Chen and Durr, 2020;Ren et al, 2020;Zhao et al, 2021;Lyu et al, 2022). However, none of the prior work includes a real-time methodology and combines the measurement of oxygenation with the measurement of fluorescence.…”

Section: Oxygenation Imagingmentioning

confidence: 94%

Trident: A dual oxygenation and fluorescence imaging platform for real-time and quantitative surgical guidance

Ségaud

Baratelli²,

Felli³

et al. 2022

Front. Photon.

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Despite recent technological progress in surgical guidance, current intraoperative assessment of tissue that should be removed (e.g., cancer) or avoided (e.g., nerves) is still performed subjectively. Optical imaging is a non-contact, non-invasive modality that has the potential to provide feedback regarding the condition of living tissues by imaging either an exogenously administered contrast agent or endogenous constituents such as hemoglobin, water, and lipids. As such, optical imaging is an attractive modality to provide physiologically and structurally relevant information for decision-making in real-time during surgery. The Trident imaging platform has been designed for real-time surgical guidance using state-of-the-art optical imaging. This platform is capable of dual exogenous and endogenous imaging owing to a unique filter and source combination, allowing to take advantage of both imaging modalities. This platform makes use of a real-time and quantitative imaging method working in the spatial frequency domain, called Single Snapshot imaging of Optical Properties (SSOP). The Trident imaging platform is designed to comply with all relevant standards for clinical use. In this manuscript, we first introduce the rationale for developing the Trident imaging platform. We then describe fluorescence and endogenous imaging modalities where we present the details of the design, assess the performance of the platform on the bench. Finally, we perform the validation of the platform during an in vivo preclinical experiment. Altogether, this work lays the foundation for translating state-of-the-art optical imaging technology to the clinic.

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“…In this study, the ischemic flap mouse model, a well-established model to examine the neovascularization and the mechanisms involved in ischemic injury ( Harder et al, 2014 ; Wu et al, 2019 ), was created to examine the functions of EV. The ischemic flap mouse model was performed as described previously ( Tepper et al, 2002 ; Lyu et al, 2022 ). Briefly, the mice were anesthetized and an incision (3 × 2 cm) was made with a three-sided full skin thickness peninsular flap on the dorsum of the mouse to create an ischemia gradient.…”

Section: Methodsmentioning

confidence: 99%

“…In the present study, the ability of EPC-EV to rescue the ischemic tissues was examined using flap mice as a model of ischemic injury ( Ichioka et al, 2002 ; Tepper et al, 2002 ; Lyu et al, 2022 ). In addition, EPC were modified to induce the secretion of a transformed EPC-EV (hwEPC-EV) with high abilities in neovascularization for the treatment of the distal ischemic tissues by systematic administration.…”

Section: Introductionmentioning

confidence: 99%

Transformed extracellular vesicles with high angiogenic ability as therapeutics of distal ischemic tissues

Ngo

Chang

Khánh

et al. 2022

Front. Cell Dev. Biol.

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Introduction: The therapeutic effects of endothelial progenitor cells (EPC) in neovascularization have been suggested; however, to date, few studies have been conducted on the ability of EPC-derived extracellular vesicles (EV) to rescue the ischemic tissues. In order to examine the functional sources of EV for cell-free therapy of ischemic diseases, we compared the functions of EPC-EV and those of Wharton’s Jelly-derived mesenchymal stem cell (WJ-EV) in the flap mouse model.Results and conclusion: Our results demonstrated that in the intravenous injection, EPC-EV, but not WJ-EV, were uptaken by the ischemic tissues. However, EPC-EV showed poor abilities to induce neovascularization and the recovery of ischemic tissues. In addition, compared to EPC-EV, WJ-EV showed a higher ability to rescue the ischemic injury when being locally injected into the mice. In order to induce the secretion of high-functional EPC-EV, EPC were internalized with hypoxic pre-treated WJ-EV, which resulted in a transformed hwEPC. In comparison to EPC, hwEPC showed induced proliferation and upregulation of angiogenic genes and miRNAs and promoted angiogenic ability. Interestingly, hwEPC produced a modified EV (hwEPC-EV) that highly expressed miRNAs related to angiogenesis, such as miR-155, miR-183, and miR-296. Moreover, hwEPC-EV significantly induced the neovascularization of the ischemic tissues which were involved in promoting the proliferation, the expression of VEGF and miR-183, and the angiogenic functions of endothelial cells. Of note, hwEPC-EV were highly uptaken by the ischemic tissues and showed a greater effect with regard to inducing recovery from ischemic injury in the intravenous administration, compared to EPC-EV. Therefore, hwEPC-EV can be considered a functional candidate for cell-free therapy to treat the distal ischemic tissues.

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The application of SFDI and LSI system to evaluate the blood perfusion in skin flap mouse model

Cited by 5 publications

References 32 publications

Laser speckle contrast analysis (LASCA): a new device in the diagnosis and monitoring of surgical treatment of hidradenitis suppurativa

Laser speckle contrast analysis (LASCA): a new device in the diagnosis and monitoring of surgical treatment of hidradenitis suppurativa

Trident: A dual oxygenation and fluorescence imaging platform for real-time and quantitative surgical guidance

Transformed extracellular vesicles with high angiogenic ability as therapeutics of distal ischemic tissues

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