The aptamers generated from HepG2 cells

Huang, Rongrong; Chen, Zhongsi; Liu, Mei; Deng, Yan; Li, Song; He, Nongyue

doi:10.1007/s11426-016-0491-7

Cited by 28 publications

(13 citation statements)

References 45 publications

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“…Compared with antibodies, aptamers possess some distinct advantages: in vitro screening of aptamers avoids ethical problems associated with animal experiments arising in antibody preparation. This further excludes the negative influences of immunogenicity and toxicity, and extends the range of targets to include metal ions, small molecules, biomacromolecules, , and even cells. , The obtained aptamer sequence can be rapidly produced by chemical synthesis with high purity, at low cost, and with minor batch-to-batch variations. , They can also be easily modified to enhance their chemical and thermal stability for long-term storage, and are labeled with various signal molecules to facilitate detection. − The antibody-based immunosensors have already been made commercially available, such as enzyme-linked immunosorbent assay (ELISA) kits and immunochromatographic strips. , Because of the numerous advantages of aptamers, they have been promising alternative molecular recognition elements to antibodies in analytics, medical diagnosis, and food safety inspection. − So it is of great significance to screen aptamers with high affinity and specificity.…”

Section: Introductionmentioning

confidence: 99%

“…This further excludes the negative influences of immunogenicity and toxicity, and extends the range of targets to include metal ions, 16 small molecules, 17 biomacromolecules, 18,19 and even cells. 20,21 The obtained aptamer sequence can be rapidly produced by chemical synthesis with high purity, at low cost, and with minor batch-to-batch variations. 22,23 They can also be easily modified to enhance their chemical and thermal stability for long-term storage, and are labeled with various signal molecules to facilitate detection.…”

Section: ■ Introductionmentioning

confidence: 99%

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Magnetic Separation-Based Multiple SELEX for Effectively Selecting Aptamers against Saxitoxin, Domoic Acid, and Tetrodotoxin

Duan

Xia

et al. 2018

J. Agric. Food Chem.

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In this study, a novel magnetic separation-based multiple systematic evolution of ligands by exponential enrichment (SELEX) was applied to select aptamers simultaneously against three kinds of marine biotoxins, including domoic acid (DA), saxitoxin (STX), and tetrodotoxin (TTX). Magnetic reduced graphene oxide (MRGO) was prepared to adsorb unbound ssDNAs and simplify the separation step. In the multiple SELEX, after the initial twelve rounds of selection against mixed targets and the subsequent four respective rounds of selection against each single target, the three resulting ssDNA pools were cloned, sequenced, and analyzed. Several aptamer candidates were selected and subjected to the binding affinity and specificity test. Finally, DA-06 ( K = 62.07 ± 19.97 nM), TTX-07 ( K = 44.12 ± 15.38 nM), and STX-41 ( K = 61.44 ± 23.18 nM) showed high affinity and good specificity for DA, TTX, and STX, respectively. They were also applied to detect and quantify DA, TTX, and STX successfully. The other two multitarget aptamers, DA-01 and TTX-27, were also obtained, which can bind with either DA or TTX. These aptamers provide alternative recognition molecules to antibodies for biosensor applications.

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Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Magnetic Separation-Based Multiple SELEX for Effectively Selecting Aptamers against Saxitoxin, Domoic Acid, and Tetrodotoxin

Duan

Xia

et al. 2018

J. Agric. Food Chem.

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“…Poly (N-vinylpyrrolidone) (PVP) was then used to stabilize PLGA nanoparticles. To improve the specificity of DOX-PLGA-PVP NPs, aptamer AS1411 was modified on the surface of DOX-PLGA-PVP NPs, to enable it to target lung cancer cells [ 148 , 149 ]. When the aptamer reacted with a receptor on the cancer surface, APT-DOX-PLGA-PVP NPs could enter the cell by endocytosis.…”

Section: Commonly Used Nanomaterials Modified With Aptamers In Cancer Therapymentioning

confidence: 99%

Applications of Aptamer-Bound Nanomaterials in Cancer Therapy

et al. 2021

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Cancer is still a major disease that threatens human life. Although traditional cancer treatment methods are widely used, they still have many disadvantages. Aptamers, owing to their small size, low toxicity, good specificity, and excellent biocompatibility, have been widely applied in biomedical areas. Therefore, the combination of nanomaterials with aptamers offers a new method for cancer treatment. First, we briefly introduce the situation of cancer treatment and aptamers. Then, we discuss the application of aptamers in breast cancer treatment, lung cancer treatment, and other cancer treatment methods. Finally, perspectives on challenges and future applications of aptamers in cancer therapy are discussed.

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“…Furthermore, tissue imaging indicated that XL-33-1 could distinguish between metastatic tumor tissues or lymph nodes and benign tissues with a detection rate of 81.7%. In our laboratory, we have recently successfully applied Cell-SELEX to develop a series of DNA aptamers specifically binding to cultured human hepatocellular carcinoma HepG2 cells [ 74 ]. The selected aptamers showed high binding affinity to HepG2 cells with K d values ranging from 46.3 to 199.4 nM and could distinguish HepG2 cells from normal human liver cells.…”

Section: Selection Of Specific Aptamers Using Selexmentioning

confidence: 99%

Aptamer selection and applications for breast cancer diagnostics and therapy

et al. 2017

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Aptamers are short non-coding, single-stranded oligonucleotides (RNA or DNA) developed through Systematic Evolution of Ligands by Exponential enrichment (SELEX) in vitro. Similar to antibodies, aptamers can bind to specific targets with high affinity, and are considered promising therapeutic agents as they have several advantages over antibodies, including high specificity, stability, and non-immunogenicity. Furthermore, aptamers can be produced at a low cost and easily modified, and are, therefore, called “chemical antibodies”. In the past years, a variety of aptamers specifically bound to both breast cancer biomarkers and cells had been selected. Besides, taking advantage of nanomaterials, there were a number of aptamer-nanomaterial conjugates been developed and widely investigated for diagnostics and targeted therapy of breast cancer. In this short review, we first present a systematical review of various aptamer selection methods. Then, various aptamer-based diagnostic and therapeutic strategies of breast cancer were provided. Finally, the current problems, challenges, and future perspectives in the field were thoroughly discussed.

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The aptamers generated from HepG2 cells

Cited by 28 publications

References 45 publications

Magnetic Separation-Based Multiple SELEX for Effectively Selecting Aptamers against Saxitoxin, Domoic Acid, and Tetrodotoxin

Magnetic Separation-Based Multiple SELEX for Effectively Selecting Aptamers against Saxitoxin, Domoic Acid, and Tetrodotoxin

Applications of Aptamer-Bound Nanomaterials in Cancer Therapy

Aptamer selection and applications for breast cancer diagnostics and therapy

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