Segregations of specific cytoplasms occur during early cleavage of the ascidian egg (Conklin, 1905) of which the most obvious is the localization of the myoplasm, presumptive for larval musculature, in the posterior cells at the four-cell stage. Cytological (Meves, 1913;Duesberg, 1915;Conklin, 1931) and cytochemical (Ries, 1937) studies indicate the localization of granules, presumably mitochondria, within the myoplasm. Recently Reverberi ( 1956) followed the distribution of mitochondria during development, using the vital stain, Janus green. The quantitative measurements of cytochrome oxidase in anterior and posterior blastomeres (Berg, 1956) gave a biochemical confirmation of the above studies as regards the localization of mitochondria.