The arabidopsis bHLH transcription factor family

Gao, Fei; Dubos, Christian

doi:10.1016/j.tplants.2023.11.022

Cited by 24 publications

(2 citation statements)

References 100 publications

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“…We focused on the bHLH TF family in C. roseus because (1) the E-box motifs, binding sites for bHLH TFs, are present in four ( T16H2 , T3O , D4H , and DAT ) of the five cold-responsive vindoline pathway gene promoters, and (2) bHLH TFs are known regulators of plant specialized metabolism and cold tolerance (Gao & Dubos, 2023; Hao, Zong, Ren, Qian, & Fu, 2021). We identified a total of 108 putative bHLH TF genes in the C. roseus genome.…”

Section: Resultsmentioning

confidence: 99%

Transcriptional reprogramming deploys a compartmentalized “timebomb” inCatharanthus roseusto fend off chewing herbivores

Liu,

Shi,

Patra

et al. 2024

Preprint

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The evolutionary arms-race between plants and insects has led to key adaptive innovations that drive diversification. Alkaloids are well-documented anti-herbivory compounds in plant chemical defenses, but how these specialized metabolites are allocated to cope with both biotic and abiotic stresses concomitantly is largely unknown. To examine how plants prioritize their metabolic resources responding to herbivory and cold, we integrated dietary toxicity bioassay in insects with co-expression analysis, hierarchical clustering, promoter assay, and protein-protein interaction in plants.Catharanthus roseus,a medicinal plant known for its insecticidal property against chewing herbivores, produces two terpenoid indole alkaloid monomers, vindoline and catharanthine. Individually, they exhibited negligible toxicity againstManduca sexta,a chewing herbivore; their condensed product, anhydrovinblastine, however, was highly toxic. Such unique insecticidal mode of action demonstrates that terpenoid indole alkaloid “timebomb” can only be activated when the two spatially isolated monomeric precursors are dimerized by herbivory. Without initial selection pressure and apparent fitness costs, this adaptive chemical defense against herbivory is innovative and sustainable.The biosynthesis of insecticidal terpenoid indole alkaloids is induced by herbivory but suppressed by cold. Here, we identified a transcription factor,Herbivore InducedVindoline-geneExpression (HIVE), that coordinates the production of terpenoid indole alkaloids in response to herbivory and cold stress. The HIVE-mediated transcriptional reprogramming allows this herbaceous perennial to allocate their metabolic resources for chemical defense at a normal temperature, when herbivory pressure is high, but switches to cold tolerance under a cooler temperature, when insect infestation is secondary.

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Section: Resultsmentioning

confidence: 99%

Transcriptional reprogramming deploys a compartmentalized “timebomb” inCatharanthus roseusto fend off chewing herbivores

Liu,

Shi,

Patra

et al. 2024

Preprint

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show abstract

“…More recently, MaNAC083 showed interaction with MaMADS1 to regulate ethylene production by modulating the transcription of MaACS1 and MaACO1/4/5/8 [ 31 ]. Moreover, studies also showed that bHLH TFs could form protein complexes with themselves or other transcriptional regulators to impact the transcriptional activity and target specificity of bHLH proteins [ 32 , 33 ]. For instance, in kiwifruit, the combination of AcMYB123 and AcbHLH42 has a substantial effect on activation of anthocyanin biosynthetic genes such as anthocyanidin synthase ( AcANS ) and UDP-3-O-galactosyltransferase 1 ( AcF3GT1 ) [ 34 ].…”

Section: Introductionmentioning

confidence: 99%

Banana MabHLH28 positively regulates the expression of softening-related genes to mediate fruit ripening independently or via cooperating with MaWRKY49/111

Wu,

Cai,

et al. 2024

Horticulture Research

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Texture softening is a physiological indicator of fruit ripening, which eventually contributes to fruit quality and consumer’s acceptance. Despite great progress has been made in identification of the genes related to fruit softening, the upstream transcriptional regulatory pathways of these softening-related genes are not fully elucidated. Here, a novel bHLH gene, designated as MabHLH28, was identified because of its significant upregulation in banana fruit ripening. DAP-Seq analysis revealed that MabHLH28 bound to the core sequence of ‘CAYGTG’ presented in promoter regions of fruit softening-associated genes, such as the genes related to cell wall modification (MaPG3, MaPE1, MaPL5, MaPL8, MaEXP1, MaEXP2, MaEXPA2 and MaEXPA15) and starch degradation (MaGWD1 and MaLSF2), and these bindings were validated by EMSA and DLR assays. Transient overexpression and knockdown of MabHLH28 in banana fruit resulted in up- and down-regulation of softening-related genes, thereby hastening and postponing fruit ripening. Furthermore, overexpression of MabHLH28 in tomato accelerated the ripening process by elevating the accumulation of softening-associated genes. In addition, MabHLH28 showed interaction withMaWRKY49/111 and itself to form protein complexes, which could combinatorically strengthen the transcription of softening-associated genes. Taken together, our findings suggest that MabHLH28 mediates fruit softening by upregulating the expression of softening-related genes either alone or in combination with MaWRKY49/111.

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Metal transport proteins and transcription factor networks in plant responses to cadmium stress

Liu,

Wen,

Cui

et al. 2024

Plant Cell Rep

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The arabidopsis bHLH transcription factor family

Cited by 24 publications

References 100 publications

Transcriptional reprogramming deploys a compartmentalized “timebomb” inCatharanthus roseusto fend off chewing herbivores

Transcriptional reprogramming deploys a compartmentalized “timebomb” inCatharanthus roseusto fend off chewing herbivores

Banana MabHLH28 positively regulates the expression of softening-related genes to mediate fruit ripening independently or via cooperating with MaWRKY49/111

Metal transport proteins and transcription factor networks in plant responses to cadmium stress

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