The Arabidopsis DREB1A gene driven by the stress-inducible rd29A promoter increases salt-stress tolerance in proportion to its copy number in tetrasomic tetraploid potato (Solanum tuberosum)

Behnam, Babak; Kikuchi, Akira; Celebi-Toprak, Fevziye; Yamanaka, Shinşuke; Kasuga, Mie; Yamaguchi‐Shinozaki, Kazuko; Watanabe, Kazuo

doi:10.5511/plantbiotechnology.23.169

Cited by 85 publications

(54 citation statements)

References 27 publications

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“…From the Southern analysis, it appeared that L1 and L2 had three copies of the gene. There was no clear reason for different number of bands using EcoRI and NSiI since AtPDC1 did not have the appropriate restriction cut sites and may be due to incomplete introgression of the transgene, i.e., the restriction enzyme can find more than one restriction site at which to cut the genome (Behnam et al 2006). L1 and L2 showed the characteristic restriction pattern, indicating that it was an independent transformation.…”

Section: Southern Analysismentioning

confidence: 99%

Alleviation of low temperature sweetening in potato by expressing Arabidopsis pyruvate decarboxylase gene and stress-inducible rd29A: A preliminary study

Pinhero

Pazhekattu

Marangoni

et al. 2011

Physiol Mol Biol Plants

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The acceptability of potatoes for processing chips and French fries is largely dependent on the color of the finished product. Most potato cultivars and varieties stored at temperatures below 9-10°C are subjected to low temperature sweetening (LTS) which result in the production of bitter-tasting, dark colored chips and French fries which are unacceptable to consumers. However, storing tubers at low temperatures (i.e., <10°C) has many advantages such as lowered weight loss during storage, natural control of sprouting, and reduction/elimination of chemical sprout inhibitors. Our earlier research results on LTS suggested a role for pyruvate decarboxylase (PDC) in LTS-tolerance. In the present study, the role of PDC was examined whereby the potato variety Snowden was transformed with Arabidopsis cold-inducible pyruvate decarboxylase gene 1 (AtPDC1) under the control of promoter rd29A. Two transgenic plants were selected and storage studies were conducted on tubers harvested from one of the transgenic lines grown under green house conditions. Transgenic tubers showed higher Agtron chip color score indicating lighter chip and lower reducing sugar and sucrose concentrations compared to the untransformed tubers during the storage periods studied at 12°C and 5°C. These results suggest that overexpression of pyruvate decarboxylase gene resulted in low temperature sweetening tolerance in the transgenic Snowden.

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Section: Southern Analysismentioning

confidence: 99%

Alleviation of low temperature sweetening in potato by expressing Arabidopsis pyruvate decarboxylase gene and stress-inducible rd29A: A preliminary study

Pinhero

Pazhekattu

Marangoni

et al. 2011

Physiol Mol Biol Plants

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“…however, transgenic plants showed growth retardation under normal growth conditions. the stress inducible expression of AtDREB1A conferred on transgenic potato plants salt and freezing stress tolerance (7,8). Similarly, Pino et al (51) reported freezing tolerance in transgenic potato that expressed AtCBF1, AtCBF2 and AtCBF3 under CaMV35S and rd29A promoters.…”

Section: Dreb Expression Confers Abiotic Stress Tolerance In Transgenmentioning

confidence: 99%

The Role of Dreb Transcription Factors in Abiotic Stress Tolerance of Plants

Khan

2011

Biotechnology & Biotechnological Equipment

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“…Belonging to the APETALA2 (AP2)/ETHYLENE RESPONSE FACTOR (ERF) family of transcription factors, DEHYDRATION RESPONSE ELEMENT B1A (DREB1A)/C-REPEAT-BINDING FACTOR3 (CBF3) is highly induced under drought stress (reviewed by Seki et al (2007)) and therefore was used to confer drought tolerance in various plants (reviewed by Umezawa et al (2006)). Overexpression of Arabidopsis thaliana AtDREB1A in potato plants was evaluated under salt and freezing stresses in in planta experiments (Celebi-Toprak et al, 2005;Behnam et al, 2006Behnam et al, , 2007. Recently, drought tolerance of AtDREB1A-expressing potato plants was carried out in an in vitro study (Huynh et al, 2014), suggesting that overexpression of AtDREB1A enhances tolerance of potato against various environmental stresses.…”

Section: Transgenics Of Drought and Heat Tolerance In Potatomentioning

confidence: 99%

Recent advances in potato genomics, transcriptomics, and transgenicsunder drought and heat stresses: a review

Aksoy

Demirel²,

Öztürk³

et al. 2015

Turk J Bot

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Sustainable potato production practices are crucial for food security and social sustainability in the future since potato is a highly nutritious food and it is considered as one of the most promising crops to reduce human hunger and poverty in the world due to its high yield potential. However, being a temperate crop, potato is exposed to various environmental stresses, including extended periods of drought and heat. The majority of potato genomics, transcriptomics, and transgenics studies concentrate on the characterization of molecular mechanisms governing cold hardiness of tubers and response and tolerance mechanisms against diseases. Likewise, potato breeding studies focus on increasing the yield, extending the postharvest storage, and developing cultivars that withstand biotic stresses. The number of genomics, transcriptomics, and transgenics studies of drought and heat tolerance in potato is limited, although they are necessary state-of-the-art research procedures to characterize and identify the regulatory mechanism underlying any stresses in order to develop new crop varieties that can tolerate harsh environmental conditions. For these reasons, this review focuses on recent advances in genomics, transcriptomics, and transgenics of drought and heat tolerance in potato.

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The Arabidopsis DREB1A gene driven by the stress-inducible rd29A promoter increases salt-stress tolerance in proportion to its copy number in tetrasomic tetraploid potato (Solanum tuberosum)

Cited by 85 publications

References 27 publications

Alleviation of low temperature sweetening in potato by expressing Arabidopsis pyruvate decarboxylase gene and stress-inducible rd29A: A preliminary study

Alleviation of low temperature sweetening in potato by expressing Arabidopsis pyruvate decarboxylase gene and stress-inducible rd29A: A preliminary study

The Role of Dreb Transcription Factors in Abiotic Stress Tolerance of Plants

Recent advances in potato genomics, transcriptomics, and transgenicsunder drought and heat stresses: a review

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