The Arabidopsis expansin gene (AtEXPA18) is capable to ameliorate drought stress tolerance in transgenic tobacco plants

Abbasi, Alireza; Malekpour, Meysam; Sobhanverdi, Sajjad

doi:10.1007/s11033-021-06589-2

Cited by 12 publications

(2 citation statements)

References 39 publications

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“…In terms of regulating plant drought stress tolerance, the overexpression of the expansin-like gene GhEXLB2 in upland cotton ( Gossypium hirsutum ) significantly enhances drought resistance [ 53 ]. The Arabidopsis expansin gene AtEXPA18 ameliorates drought stress tolerance in transgenic tobacco plants [ 54 ]. The Brassica rapa expansin-like B1 gene, BrEXLB1 , is involved in regulating root development and drought stress responses [ 55 ].…”

Section: Discussionmentioning

confidence: 99%

Overexpression of Wild Soybean Expansin Gene GsEXLB14 Enhanced the Tolerance of Transgenic Soybean Hairy Roots to Salt and Drought Stresses

Wang,

Zhang,

et al. 2024

Plants

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As a type of cell-wall-relaxing protein that is widely present in plants, expansins have been shown to actively participate in the regulation of plant growth and responses to environmental stress. Wild soybeans have long existed in the wild environment and possess abundant resistance gene resources, which hold significant value for the improvement of cultivated soybean germplasm. In our previous study, we found that the wild soybean expansin gene GsEXLB14 is specifically transcribed in roots, and its transcription level significantly increases under salt and drought stress. To further identify the function of GsEXLB14, in this study, we cloned the CDS sequence of this gene. The transcription pattern of GsEXLB14 in the roots of wild soybean under salt and drought stress was analyzed by qRT-PCR. Using an Agrobacterium rhizogenes-mediated genetic transformation, we obtained soybean hairy roots overexpressing GsEXLB14. Under 150 mM NaCl- and 100 mM mannitol-simulated drought stress, the relative growth values of the number, length, and weight of transgenic soybean hairy roots were significantly higher than those of the control group. We obtained the transcriptomes of transgenic and wild-type soybean hairy roots under normal growth conditions and under salt and drought stress through RNA sequencing. A transcriptomic analysis showed that the transcription of genes encoding expansins (EXPB family), peroxidase, H+-transporting ATPase, and other genes was significantly upregulated in transgenic hairy roots under salt stress. Under drought stress, the transcription of expansin (EXPB/LB family) genes increased in transgenic hairy roots. In addition, the transcription of genes encoding peroxidases, calcium/calmodulin-dependent protein kinases, and dehydration-responsive proteins increased significantly. The results of qRT-PCR also confirmed that the transcription pattern of the above genes was consistent with the transcriptome. The differences in the transcript levels of the above genes may be the potential reason for the strong tolerance of soybean hairy roots overexpressing the GsEXLB14 gene under salt and drought stress. In conclusion, the expansin GsEXLB14 can be used as a valuable candidate gene for the molecular breeding of soybeans.

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Section: Discussionmentioning

confidence: 99%

Overexpression of Wild Soybean Expansin Gene GsEXLB14 Enhanced the Tolerance of Transgenic Soybean Hairy Roots to Salt and Drought Stresses

Wang,

Zhang,

et al. 2024

Plants

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“…Expansins, also known as expansion proteins, are nonenzymatic proteins that induce cell wall extension and relaxation in acidic environments (Mcqueen-Mason et al, 1992). They encode gene superfamily containing four subfamilies: α-expansin (EXPA), β-expansin (EXPB), expansin-like A (EXLA) and expansin-like B (EXLB) (Alireza et al, 2021;Kende et al, 2004;Sun et al, 2021). Among them, EXPA and EXPB are the largest and most diverse subfamilies, and their mode of action has been extensively studied (Ana et al, 2022;Marowa et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Overexpression of the TaEXPA19 gene improves low‐temperature tolerance in winter wheat (Triticum aestivum)

Li,

Hu,

Peng

et al. 2023

Plant Breeding

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Low temperature is one of the main abiotic stresses that affects plant growth, causing serious damage or even death to plants. The differential expression of the TaEXPA19 gene in the above and underground parts of winter wheat and the implications for cold resistance remain unclear. In this study, the TaEXPA19 gene was cloned and analysed for expression in winter wheat, and transgenic Arabidopsis thaliana was constructed to investigate the effect of the TaEXPA19 gene in response to low‐temperature stress on plant growth. The TaEXPA19‐A and TaEXPA19‐D genes have different response patterns in the above and underground parts of transgenic A. thaliana. When plants were subjected to low‐temperature stress, the leaves were quickly upregulated and the roots were downregulated, and then upregulated to respond to low‐temperature stress to promote the growth of leaf length and leaf width petiole length. The results indicated that TaEXPA19 genes could improve low‐temperature tolerance in plants. The results of this study laid a foundation for the study of the cold resistance of winter wheat.

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Overexpression of the GmEXPA1 gene reduces plant susceptibility to Meloidogyne incognita

et al. 2022

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The Arabidopsis expansin gene (AtEXPA18) is capable to ameliorate drought stress tolerance in transgenic tobacco plants

Cited by 12 publications

References 39 publications

Overexpression of Wild Soybean Expansin Gene GsEXLB14 Enhanced the Tolerance of Transgenic Soybean Hairy Roots to Salt and Drought Stresses

Overexpression of Wild Soybean Expansin Gene GsEXLB14 Enhanced the Tolerance of Transgenic Soybean Hairy Roots to Salt and Drought Stresses

Overexpression of the TaEXPA19 gene improves low‐temperature tolerance in winter wheat (Triticum aestivum)

Overexpression of the GmEXPA1 gene reduces plant susceptibility to Meloidogyne incognita

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