2002
DOI: 10.1105/tpc.010346
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The Arabidopsis SPIKE1 Gene Is Required for Normal Cell Shape Control and Tissue Development

Abstract: Regulated growth and cell shape control are fundamentally important to the function of plant cells, tissues, and organs. The signal transduction cascades that control localized growth and cell shape, however, are not known. To better understand the relationship between cytoskeletal organization, organelle positioning, and regulated vesicle transport, we conducted a forward genetic screen to identify genes that regulate cytoskeletal organization in plants. Because of the distinct requirements for microtubules a… Show more

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Cited by 209 publications
(219 citation statements)
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“…The presence of more than 14 ROP-GEFs in Arabidopsis is suggested by the existence of a protein called SPIKE, which was identified from one of the many trichome mutants. SPIKE is also necessary for pavement cell lobe formation and shows characteristics of a rho-GEF [43]. In animal cells, GEF-H1 is believed to be activated by microtubule depolymerization [44]; if one of the plant GEFs behaves similarly, this would reinforce the model of Fu et al [41 ] by enhancing ROP activity in the lobes, where microtubules are sparse.…”
Section: References and Recommended Readingsupporting
confidence: 67%
“…The presence of more than 14 ROP-GEFs in Arabidopsis is suggested by the existence of a protein called SPIKE, which was identified from one of the many trichome mutants. SPIKE is also necessary for pavement cell lobe formation and shows characteristics of a rho-GEF [43]. In animal cells, GEF-H1 is believed to be activated by microtubule depolymerization [44]; if one of the plant GEFs behaves similarly, this would reinforce the model of Fu et al [41 ] by enhancing ROP activity in the lobes, where microtubules are sparse.…”
Section: References and Recommended Readingsupporting
confidence: 67%
“…We analyzed pin1-1 plants for pavement cell shape defects at 2, 5, and 10 DAG (Fig. 1); 10 DAG is the terminal phenotype at which cotyledon expansion ceases (Qiu et al, 2002). We used the recently described LobeFinder algorithm to measure cell shape and count lobes because it eliminates the unavoidable variability in lobe number scoring among individuals (Wu et al, 2016).…”
Section: Genetic Analysis Of the Plasma Membrane-localized Pinsmentioning
confidence: 99%
“…However, longitudinal alignment of microtubules along the anticlinal wall is a permissive arrangement for transverse elongation of the anticlinal wall in the plane of the epidermis. For example, longitudinally aligned microtubules are observed not only at the convex region of lobes but also along the growing flanks and tips of pavement cell protrusions (Qiu et al, 2002;Zhang et al, 2011). An alternative model proposes that this arrangement of microtubules and microfibrils could enable increased local strain to accommodate the polarized expansion of both cells at the lobing interface (Szymanski, 2014).…”
mentioning
confidence: 99%
“…RT-PCR was performed as before (Qiu et al, 2002) using the following primers: MKK1-F, 5#-AGCGAGTTCTTCGAGGTCTTTG-3#; MKK1-R, 5#-AGCAAGT-GGGGGAATCAAAGATC-3#; MKK2-F, 5#-CATCCCTGACTCCTATCTTTCTG-3#; MKK2-R, 5#-CGATCCTGCATCTGTGAAGTAG-3#; ACT7-F, 5#-GTGAGGA-TATTCAGCCACTTGTCTG-3#; and ACT7-R, 5#-TGTGAGATCCCGACCCG-CAAGATC-3#.…”
Section: Rna Isolation Rt-pcr and Real-time Rt-pcrmentioning
confidence: 99%